Abstract: The nitrate transporter 1/peptide transporter family（NRT1/PTR family，NPF）plays a crucial role in nitrogen uptake and utilization in plants，and is also involved in diverse biotic and abiotic stress responses.To elucidate the function of NPF in the waterlogging stress of sesame（Sesamum indicum L.），a systematic bioinformatics analysis was conducted to identify SiNPF genes. Furthermore，the dynamic expression profiles of selected SiNPFs under submergence conditions were characterized.The results of genome⁃wide analysis indicated that a total of 66 SiNPF members were identified in sesame，distributed across 12 chromosomes（excluding chr13），with chr2（12 genes），chr6（11），chr8（9），and chr9（8）being gene⁃enriched regions.And after constructing the phylogenetic tree（Mega 7，NJ method），it was found that these genes were divided into 10 subfamilies.The analysis of gene structure and conserved motif revealed that the majority of SiNPFs had similar gene structures and high conservation，with 44 SiNPFs containing all conserved motifs.Protein property analysis indicated that SiNPF proteins ranged in molecular weight from 20.96 to 135.01 ku，with the majority being alkaline（48 proteins）and stable（52），all of which were hydrophobic（GRAVY>0）.Transmembrane topology prediction（TMHMM）showed that these proteins contained multiple transmembrane domains（4 to 17）.The prediction results of the cis acting elements of the promoters showed a high frequency of cis⁃acting elements，including light⁃responsive elements，and ABA⁃responsive，anaerobically induced，and methyl jasmonate⁃responsive elements.Transcriptome analysis revealed that under waterlogging stress，15 SiNPFs were significantly downregulated in the roots of sesame varieties C177 and C185，while SiNPF2，SiNPF48，and SiNPF56 were significantly upregulated.The results of cloning and sequencing of these three significantly upregulated SiNPFs from varieties C177 and C185 showed that the amino acid sequence differences between the two varieties were limited，and the overall similarity was high. RT⁃qPCR validation confirmed that the expression patterns of these three genes under waterlogging stress were highly consistent with the transcriptome sequencing results.These findings indicate that specific SiNPF genes are closely associated with waterlogging response in sesame，providing a theoretical basis for further investigation on the biological functions of SiNPF genes and breeding of new waterlogging⁃tolerant sesame varieties.

Key words: Sesame, SiNPF, Waterlogging stress, Bioinformatics analysis, Expression analysis

摘要： 硝酸盐转运蛋白1/肽转运蛋白家族（NRT1/PTR family，NPF）在植物氮素吸收利用中发挥着重要作用，同时参与多种生物、非生物胁迫响应。为解析芝麻（Sesamum indicum L.）NPF在渍害胁迫中的功能，通过生物信息学方法首次系统鉴定了芝麻NPF基因（SiNPF），并部分阐明其在渍害胁迫下的动态表达特征。全基因组分析结果表明，在芝麻中共鉴定出66 个SiNPF成员，分布于芝麻12 条染色体上（chr13除外），其中chr2（12个）、chr6（11个）、chr8（9个）和chr9（8个）为基因富集区。系统进化树构建（Mega 7，NJ法）结果将其划分为10个亚家族。基因结构和保守基序分析发现，多数SiNPF 基因结构相似，且具有较高的保守性，其中44个SiNPF包含所有保守基序。蛋白质特性分析揭示，SiNPF蛋白分子质量集中于20.96~135.01 ku，多数为碱性（48个）和稳定（52个）蛋白质，全部为疏水蛋白质（GRAVY>0）。跨膜拓扑预测（TMHMM）结果表明，SiNPF蛋白包含多个跨膜结构（4~17个）。启动子顺式作用元件预测结果显示，基因启动子上游光响应元件和脱落酸响应、厌氧诱导及茉莉酸甲酯响应元件分布频率较高。转录组分析发现，渍害胁迫下，15 个SiNPF在芝麻品种C177 和C185 根部显著下调表达，而SiNPF2、SiNPF48 和SiNPF56 显著上调表达。分别克隆芝麻品种C177和C185中上述3个显著上调的SiNPF 基因，并测序比对，发现2个品种氨基酸序列差异有限，整体上相似度较高。RT-qPCR验证表明，3个基因在渍害胁迫下的表达模式与转录组测序结果高度一致。可见，部分SiNPF基因与芝麻的渍害响应存在密切关系，为进一步探究SiNPF基因的生物学功能及培育芝麻耐渍新品种提供了理论依据。

关键词: 芝麻, SiNPF, 渍害胁迫, 生物信息学分析, 表达分析