The Application Effect of Immobilized Mushroom Strains of Pleurotus eryngii in Cultivation Production

doi:10.15933/j.cnki.1004-3268.2025.05.014

Abstract

Abstract: The preliminary research indicated that immobilized strains could still maintain high biological activity after storage at 20 ℃ and 4 ℃ for 30 days.In order to verify the application effect of immobilized strains in production practice，immobilized strains of different storage time were inoculated into Pleurotus eryngii cultivation bags，with fresh liquid strains as the control.The growth rate of Pleurotus eryngii mycelium，the time of mycelium filling bag，the contamination rate of cultivation bag，the total yield per cultivation bag at different storage periods were measured，and the biological efficiency and commodity rate were calculated.Nutrients such as ash，protein and fat were analyzed.The results indicated that the fruiting body morphology，cap diameter，stem diameter，and stem length of the cultivation bags inoculated with immobilized strains stored for 30 days at 20℃ and 4℃ showed no significant differences compared to the control.With the increase of storage time，the filling time of mycelium slowly increased，and the growth rate of mycelium gradually slowed down.The contamination rate of the bag was less affected by storage time and temperature.The total yield per cultivation bag was higher than 561.00 g/bag，the biological efficiency was higher than 122.00%，and the commodity rate exceeded 82.00%，meeting the production requirements. In addition，there were no significant differences in ash，protein，fat and other nutrients in the fruit body of Pleurotus eryngii.Therefore，the immobilized strains stored for 30 days can be used in the cultivation and production of Pleurotus eryngii，providing a feasible technical solution to the storage and transportation challenges of Pleurotus eryngii strains.

Key words: Pleurotus eryngii, Liquid strain, Immobilization, Cultivation, Biological efficiency

摘要： 前期研究表明，固定化菌种在20 ℃和4 ℃条件下保藏30 d仍具有较高的生物活性。为验证固定化菌种在生产实践中的应用效果，将不同保藏时间的固定化菌种接种到杏鲍菇栽培袋中，以新鲜液体菌种为对照，测定不同保藏时间的杏鲍菇菌丝生长速度、菌丝满袋时间、栽培袋污染率、每栽培袋总产量，计算生物学效率和商品率，并测定灰分、蛋白质、脂肪等营养成分含量。结果表明，接种在20 ℃和4 ℃条件下保藏30 d的固定化菌种栽培袋的子实体形态、菌盖直径、菌柄直径、菌柄长度等指标均与液体菌种对照无显著差异；随着保藏时间增加，菌丝满袋时间缓慢增加，菌丝生长速度逐渐变慢；栽培袋污染率受保藏时间及温度影响较小；栽培袋总产量均高于561.00 g/袋，生物学效率均高于122.00%，商品率均超过82.00%，达到了生产要求；杏鲍菇子实体的灰分、蛋白质、脂肪等营养成分与对照均无显著差异。因此，保藏30 d的固定化菌种可用于杏鲍菇栽培生产。

关键词: 杏鲍菇, 液体菌种, 固定化, 栽培, 生物学效率

CLC Number:

S646

ZHOU Tingting, JIANG Ran, ZHU Haoran, MA Guizhen. The Application Effect of Immobilized Mushroom Strains of Pleurotus eryngii in Cultivation Production[J]. Journal of Henan Agricultural Sciences, 2025, 54(5): 124-129.

周婷婷, 蒋冉, 朱浩然, 马桂珍. 杏鲍菇固定化菌种在栽培生产中的应用效果[J]. 河南农业科学, 2025, 54(5): 124-129.

Figures/Tables 8

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