关键词: Cd胁迫, 马铃薯, 根际土壤, 聚类分析, 高通量测序, 相对丰度, 细菌群落多样性, 耐性菌

Abstract: In order to reveal the difference of bacterial community structure in rhizosphere soil and the change characteristics of rhizosphere bacteria under Cd stress,and understand the relationship between bacteria and Cd tolerance in potato rhizosphere soil,the bacterial diversity and community structure of potato rhizosphere soil under Cd stress with different concentrations(0,10,50,100 mg/kg)were studied by high throughput sequencing.The results showed that the bacterial diversity of potato rhizosphere soil was the lowest when the Cd stress concentration was 100 mg/kg,and the highest when the Cd stress concentration was 10 mg/kg.Proteobacteria was the dominant phylum at phyla level, accounting for 37.78%—48.28%.At genus level, Mizugakiibacter was dominant, accounting for 9.16%—20.44%.Principal component analysis showed that the community structure of bacteria in potato rhizosphere soil under Cd stress was significantly different from that of control.Three genera of Cd-tolerant bacteria,Brubacter,Flexivirga and Jatrophilhabitans,were identified by correlation analysis.

Key words: Cd stress;Potato, Rhizosphere soil;Cluster analysis;High-throughput sequencing;Relative abundance;Bacterial community diversity;Tolerant bacterium