外源硒添加对香菇金属元素累积与食用安全性的影响

doi:10.15933/j.cnki.1004-3268.2025.09.018

河南农业科学 ›› 2025, Vol. 54 ›› Issue (9): 171-180.DOI: 10.15933/j.cnki.1004-3268.2025.09.018

• 农业信息与工程·农产品加工 • 上一篇

外源硒添加对香菇金属元素累积与食用安全性的影响

段然^1,2，郭洁^1,2，周晓华^1,2，张迪^1,2，马莹^1,2，吴绪金^1,2，王铁良^1,2

（1.河南省农业科学院农产品质量安全研究所/河南省农产品质量安全与加工重点实验室，河南郑州 450002；2.农业农村部农产品质量安全风险评估实验室（郑州），河南郑州 450002）

收稿日期:2025-03-10 接受日期:2025-05-02 出版日期:2025-09-15 发布日期:2025-09-16
通讯作者: 王铁良（1978-），男，河南柘城人，副研究员，硕士，主要从事农产品质量安全研究。E-mail：wangtieliang@hnagri.org.cn
作者简介:段然（1991-），女，河南濮阳人，助理研究员，博士，主要从事农产品质量安全研究。E-mail：yucca1130@163.com
基金资助:
河南省科技攻关项目（252102110080，252102320364，242102110314，232102110165）；河南省农业科学院自主创新基金资助项目（2025ZC144）

Effects of Exogenous Selenium Addition on Metal Element Accumulation and Edible Safety of Lentinula edodes

DUAN Ran^1,2，GUO Jie^1,2，ZHOU Xiaohua^1,2，ZHANG Di^1,2，MA Ying^1,2，WU Xujin^1,2，WANG Tieliang^1,2

（1.Institute of Quality and Safety for Agro‑products/Henan Provincial Key Laboratory of Quality Safety and Processing for Agro‑products，Henan Academy of Agricultural Sciences，Zhengzhou 450002，China；2.Risk Assessment Laboratory of Agricultural Products Quality and Safety of Ministry of Agriculture and Rural Affairs（Zhengzhou），Zhengzhou 450002，China）

Received:2025-03-10 Accepted:2025-05-02 Published:2025-09-15 Online:2025-09-16

摘要/Abstract

摘要： 为探究外源硒对香菇（Lentinula edodes）子实体硒富集特性及金属元素累积的影响，以香菇品种向阳二号和泌花一号为研究对象，设置0~40 mg/kg外源硒添加质量分数梯度，测定子实体中硒、必需金属元素（锌、铁、锰、铜、铬、镍）及有毒元素（镉、砷、铅、汞）含量，结合Pearson相关性与主成分分析法解析元素互作关系，并基于估计日摄入量（EDI）和健康风险指数（HRI）评估膳食暴露风险。结果表明，子实体硒累积量与外源硒添加量呈显著的线性正相关关系（R^²≥0.985），生物富集系数均>3.10，当添加量为40 mg/kg时，子实体硒累积量分别高达147.42 mg/kg（向阳二号）和124.22 mg/kg（泌花一号）。香菇子实体中锌、铁和锰基础累积量较高；外源硒显著提高了向阳二号子实体中铜累积量，显著降低了泌花一号子实体的锰累积量；且向阳二号的镍累积量随硒添加量增加呈先升高后降低趋势。当外源硒添加量≤2 mg/kg时，子实体对砷、铅的累积显著降低；添加量≥6 mg/kg时，子实体对汞累积量随外源硒添加量增加而降低（降幅20.00%~95.00%）。元素互作分析显示，硒与铬在0.001水平上呈显著正相关（r=0.78），与汞在0.001水平上呈显著负相关（r=-0.73）。健康风险评估表明，外源硒添加量≤2 mg/kg时，子实体中硒的HRI均<1，符合安全阈值，必需金属元素HRI均≤0.164，有毒元素HRI均<1，风险顺序为砷>镉>铅≈汞。综上，适量添加外源硒（≤2 mg/kg）不仅能实现香菇的高效硒生物强化，协同调控必需金属元素的平衡，而且能显著抑制部分有毒元素的累积，从而有效保障了富硒香菇的食用安全性和营养价值。

关键词: 香菇, 外源硒, 金属元素, 相关性分析, 风险评估

Abstract: To investigate the effects of exogenous selenium on the selenium accumulation characteristics and metal element accumulation in the fruiting bodies of Lentinula edodes，two L.edodes varieties，Xiangyang No.2 and Bihua No.1，were used as experimental subjects. A mass fraction gradient of 0—40 mg/kg of exogenous selenium was applied，and the content of selenium，essential metal elements（zinc，iron，manganese，copper，chromium，and nickel），as well as toxic elements（cadmium，arsenic，lead，and mercury）in the fruiting bodies was determined.Pearson correlation analysis and principal component analysis were employed to elucidate the interactions between these elements. Dietary exposure risks were assessed based on estimated daily intake（EDI）and health risk indices（HRI）.The results showed that there was a significant linear positive correlation between the accumulation of selenium in fruiting bodies and the amount of exogenous selenium addition（R²≥0. 985），and the bio‑concentration factors were all >3.10.When the addition amount was 40 mg/kg，the accumulation of selenium in fruiting bodies reached 147.42 mg/kg（Xiangyang No.2）and 124.22 mg/kg（Bihua No.1），respectively.The basic accumulation level of zinc，iron and manganese in the fruiting body of L. edodes was higher. Exogenous selenium significantly increased the accumulation of copper in the fruiting body of Xiangyang No.2，and significantly reduced the accumulation of manganese in the fruiting body of Bihua No.1.Additionally，the nickel accumulation in Xiangyang No.2 fruiting bodies showed a trend of first increasing and then decreasing with increasing of selenium addition.When the exogenous selenium addition was ≤2 mg/kg，the accumulation of arsenic and lead in fruiting bodies decreased significantly.When the addition was ≥6 mg/kg，the accumulation of mercury in fruiting bodies decreased with the increase of exogenous selenium addition（decreased by 20.00%—95.00%）.Element interaction analysis showed that selenium was positively correlated with chromium at the 0. 001 level（r=0.78），and negatively correlated with mercury at the 0.001 level（r=-0.73）.Health risk assessment indicated that when the exogenous selenium addition was ≤2 mg/kg，the HRI of selenium in the fruiting bodies was <1，which met the safety threshold.The HRIs of essential metal elements were ≤0.164，and the HRIs of toxic elements were <1.The risk order was arsenic>cadmium>lead≈mercury. In summary，appropriate amount of exogenous selenium（≤2 mg/kg）can not only achieve high‑efficiency selenium biofortification of L.edodes，synergistically regulate the balance of essential metal elements，but also significantly inhibit the accumulation of some toxic elements，thus effectively ensuring the edible safety and nutritional value of selenium‑enriched L.edodes.

Key words: Lentinula edodes, Exogenous selenium, Metal element, Correlation analysis, Risk assessment

中图分类号:

S646.1⁺2

段然, 郭洁, 周晓华1, 张迪, 马莹, 吴绪金, 王铁良. 外源硒添加对香菇金属元素累积与食用安全性的影响[J]. 河南农业科学, 2025, 54(9): 171-180.

DUAN Ran, GUO Jie, ZHOU Xiaohua, ZHANG Di, MA Ying, WU Xujin, WANG Tieliang. Effects of Exogenous Selenium Addition on Metal Element Accumulation and Edible Safety of Lentinula edodes[J]. Journal of Henan Agricultural Sciences, 2025, 54(9): 171-180.

图/表 7

参考文献

［1］XU Z N，LIN Z Q，ZHAO G S，et al. Biogeochemical behavior of selenium in soil‑air‑water environment and its effects on human health［J］. International Journal of Environmental Science and Technology，2024，21（1）：1159‑1180.
［2］BIRRINGER M，PILAWA S，FLOHÉ L.Trends in selenium biochemistry［J］. Natural Product Reports，2002，19（6）：693‑718.

［3］FILIPPINI T，FAIRWEATHER‑TAIT S，VINCETI M.Selenium and immune function：A systematic review and meta‑analysis of experimental human studies［J］.The American Journal of Clinical Nutrition，2023，117（1）：93‑110.

［4］HUANG L J，MAO X T，LI Y Y，et al. Multiomics analyses reveal a critical role of selenium in controlling T cell differentiation in Crohn’s disease［J］. Immunity，2021，54（8）：1728‑1744.

［5］DABRAVOLSKI S A， SUKHORUKOV V N，MELNICHENKO A A，et al. The role of selenium in atherosclerosis development，progression，prevention and treatment［J］. Biomedicines，2023，11（7）：2227‑9059.

［6］中国营养学会膳食营养素参考摄入量2023专家委员会.《中国居民膳食营养素参考摄入量》2023修订版简介［J］. 营养学报，2023，45（6）：521‑524.

Expert Committee on Dietary Nutrient Reference Intakes 2023 of the Chinese Nutrition Society. Brief introduction to the 2023 revised edition of Chinses Dietary Nutrient Reference Intakes［J］. Acta Nutrimenta Sinica，2023，45 （6）：521‑524.

［7］SHAHID M，NIAZI N K，KHALID S，et al. A critical review of selenium biogeochemical behavior in soil‑plant system with an inference to human health［J］.Environmental Pollution，2018，234：915‑934.

［8］YANG H，YANG X F，NING Z P，et al.The beneficial and hazardous effects of selenium on the health of the soil‑plant‑human system：An overview［J］. Journal of Hazardous Materials，2022，422：126876.

［9］D’AMATO R，REGNI L，FALCINELLI B，et al. Current knowledge on selenium biofortification to improve the nutraceutical profile of food：A comprehensive review［J］. Journal of Agricultural and Food Chemistry，2020，68（14）：4075‑4097.

［10］HU T，LI L，HUI G F，et al. Selenium biofortification and its effect on multi‑element change in Auricularia auriculat［J］. Food Chemistry，2019，295：206‑213.

［11］KAŁA K，KRAKOWSKA A，SZEWCZYK A，et al.Determining the amount of potentially bioavailable phenolic compounds and bioelements in edible mushroom mycelia of Agaricus bisporus，Cantharellus cibarius，and Lentinula edodes［J］.Food Chemistry，2021，352：129456.

［12］ASSUNÇÃO L S，SILVA M C S，FERNANDEZ M G，et al. Speciation of selenium in Pleurotus ostreatus and Lentinula edodes mushrooms［J］.Journal of Biotechnology Letters，2014，5（1）：79‑86.

［13］ZHOU F，YANG W X，WANG M K，et al. Effects of selenium application on Se content and speciation in Lentinula edodes［J］. Food Chemistry，2018，265：182‑188.

［14］李红英，陈菲菲，孙举志，等. 硒对香菇品质的影响［J］.北方园艺，2022（12）：106‑113.

LI H Y，CHEN F F，SUN J Z，et al. Effects of selenium on Lentinus edodes quality［J］. Northern Horticulture，2022（12）：106‑113.

［15］FALANDYSZ J，BOROVIČKA J. Macro and trace mineral constituents and radionuclides in mushrooms：Health benefits and risks［J］. Applied Microbiology and Biotechnology，2013，97（2）：477‑501.

［16］CHEN S Z，GUO Q Z，ZHOU T H，et al. Levels and health risk assessment of inorganic arsenic，ethylmercury，and heavy metals in edible mushrooms collected from online supermarket in China［J］.Biological Trace Element Research，2024，202（4）：1802‑1815.

［17］YU H L，SHEN X F，CHEN H Y，et al. Analysis of heavy metal content in Lentinula edodes and the main influencing factors［J］. Food Control，2021，130：108198.

［18］STOJEK K，CZORTEK P，BOBROWSKA‑KORCZAK B，et al.Fungal species and element type modulate the effects of environmental factors on the concentration of potentially toxic elements in mushrooms［J］.Environmental Pollution，2024，353：124152.

［19］MA Y Z，GUO F X，ZHU H D，et al. Risk assessment and impact prediction of associated heavy metal pollution in selenium‑rich farmland［J］. Science of the Total Environment，2024，950：175321.

［20］ZHANG L Q，SONG H X，GUO Y B，et al. Benefit‑risk assessment of dietary selenium and its associated metals intake in China（2017‑2019）：Is current selenium‑rich agro‑food safe enough？［J］.Journal of Hazardous Materials，2020，398：123224.

［21］HU T，LI H F，ZHAO G S，et al. Selenium enriched Hypsizygus marmoreus，a potential food supplement with improved Se bioavailability［J］. LWT，2021，140：110819.

［22］DOWLATI M，SOBHI H R，ESRAFILI A，et al. Heavy metals content in edible mushrooms：A systematic review，meta‑analysis and health risk assessment［J］.Trends in Food Science & Technology，2021，109：527‑535. ［23］GUAN H R，ZHANG J W，WANG T，et al. Lowered oral bioaccessibility of cadmium and selenium and associated health risk by co‑digestion of rice and vegetables［J］. Science of the Total Environment，2023，898：165489.

［24］QING Y，LI Y Z，CAI X Y，et al. Assessment of cadmium concentrations in foodstuffs and dietary exposure risk across China：A metadata analysis［J］.Exposure and Health，2023，15（4）：951‑961.

［25］KOUTROTSIOS G，DANEZIS G，GEORGIOU C，et al.Elemental content in Pleurotus ostreatus and Cyclocybe cylindracea mushrooms：Correlations with concentrations in cultivation substrates and effects on the production process［J］. Molecules，2020，25（9）：2179.

［26］ZHOU F，DINH Q T，YANG W X，et al. Assessment of speciation and in vitro bioaccessibility of selenium in Se‑enriched Pleurotus ostreatus and potential health risks［J］. Ecotoxicology and Environmental Safety，2019，185：109675.

［27］梁郅哲，司振兴，牛慧伟，等. 硒对植物的毒害作用研究进展［J］. 河南农业科学，2022，51（6）：13‑21.

LIANG Z Z，SI Z X，NIU H W，et al. Research progress on toxic effect of selenium on plants［J］.Journal of Henan Agricultural Sciences，2022，51（6）：13‑21.

［28］KIELISZEK M，BŁAŻEJAK S，BZDUCHA‑WRÓBEL A，et al. Effect of selenium on growth and antioxidative system of yeast cells［J］. Molecular Biology Reports，2019，46（2）：1797‑1808.

［29］XU M M，ZHU S，LI Y R，et al. Effect of selenium on mushroom growth and metabolism：A review［J］.Trends in Food Science & Technology，2021，118：328‑340.

［30］张爱琴，郭斌，柳利龙，等. 基于主成分分析和隶属函数法的谷子营养品质综合评价［J］. 河南农业科学，2025，54（1）：28‑39.
ZHANG A Q，GUO B，LIU L L，et al. Comprehensive evaluation of nutritional quality of foxtail millet based
on principal component analysis and membership function method［J］. Journal of Henan Agricultural Sciences，2025，54（1）：28‑39.

［31］ZHOU X B，YANG J，KRONZUCKER H J，et al.Selenium biofortification and interaction with other elements in plants：A review［J］. Frontiers in Plant Science，2020，11：586421.

［32］DONG Z，XIAO Y Q，WU H. Selenium accumulation，speciation，and its effect on nutritive value of Flammulina velutipes（Golden needle mushroom）［J］.Food Chemistry，2021，350：128667.

［33］VISHWAKARMA S，GENU DALBHAGAT C，MANDLIYA S，et al. Investigation of natural food fortificants for improving various properties of fortified foods：A review［J］. Food Research International，2022，156：111186. ［34］张盼盼，邵运辉，李春华，等.氮锌配施对不同锌效率玉米品种不同粒位籽粒矿质元素累积分配的影响［J］. 河南农业科学，2025，54（4）：11‑26.
ZHANG P P，SHAO Y H，LI C H，et al. Effect of nitrogen and zinc application on accumulation and distribution of mineral elements in grain at different positions of ear of maize with different Zn efficiency［J］. Journal of Henan Agricultural Sciences，2025，54 （4）：11‑26.

［35］CHEN H G，CHEN Y J，CHEN C，et al. Reproducibility of essential elements chromium，manganese，iron，zinc and selenium in spot samples，first‑morning voids and 24‑h collections from healthy adult men［J］.British Journal of Nutrition，2019，122（3）：343‑351.

［36］ HUANG L J，HE F，WU B Y. Mechanism of effects of nickel or nickel compounds on intestinal mucosal barrier［J］. Chemosphere，2022，305：135429.

［37］MAN S，ZHENG F Y，LI S X，et al. Benefit‑risk assessment of metal bioavailability in edible fungi by biomimetic whole digestive tracts with digestion，metabolism，and absorption functions［J］. Journal of Hazardous Materials，2021，416：126146.

［38］SEYFFERTH A L，MCCLATCHY C，PAUKETT M.Arsenic，lead，and cadmium in U. S. mushrooms and substrate in relation to dietary exposure［J］.Environmental Science & Technology，2016，50（17）：9661‑9670.

［39］DE OLIVEIRA A P，NAOZUKA J，LANDERO FIGUEROA J A. Feasibility study for mercury remediation by selenium competition in Pleurotus mushrooms［J］. Journal of Hazardous Materials，2023，451：131098.

［40］WU Y S，ZHAI D D，JIANG N，et al. Proteomics and physiologic analysis reveal different response strategies to cadmium stress in Lentinula edodes［J］.Food Chemistry，2025，487：144739.

［41］WANG Y，WANG X T，LAN W Q，et al. Impacts and tolerance responses of Coprinus comatus and Pleurotus cornucopiae on cadmium contaminated soil ［J］.Ecotoxicology and Environmental Safety，2021，211：111929.

[1]	王文霞, 刘宇琪, 夏清, 智慧, 杜杰. 外源硒对干旱胁迫下胡麻生理特性的影响[J]. 河南农业科学, 2025, 54(7): 55-63.
[2]	谢国雪, 黄启厅, 覃泽林, 兰宗宝, 梁永检, 温国泉, 杨绍锷, 宁夏. 遥感技术支持下的柑橘黄龙病受灾风险评估[J]. 河南农业科学, 2024, 53(9): 141-149.
[3]	刘丽娜, 崔国梅, 李顺峰, 许方方, 田广瑞, 纪沐风, 魏书信, 王安建. 香菇菌糠中木质纤维素的挤压降解及结构表征[J]. 河南农业科学, 2024, 53(8): 173-180.
[4]	刘丽娜, 李顺峰, 许方方, 崔国梅, 高帅平, 王安建, 田广瑞, 魏书信. 不同生长期香菇培养料中木质纤维素的变化特征[J]. 河南农业科学, 2024, 53(3): 110-117.
[5]	张林霞, 张蔚, 张书婷, 孙苗苗, 张晓敏, 李志能, 刘国锋. 矮牵牛花朵大小及相关性状遗传分析[J]. 河南农业科学, 2024, 53(2): 118-127.
[6]	魏书信, 王安建, 高帅平, 田广瑞, 刘丽娜, 许方方, 李顺峰, 崔国梅. 香菇培养料的双螺杆挤压灭菌工艺优化[J]. 河南农业科学, 2024, 53(1): 172-179.
[7]	王晓洁, 黎美霞, 陶蕾, 张波, 何昕孺, 米佳, 戴国礼, 徐文娣. 48 份黑果枸杞种质主要表型和品质性状的遗传多样性研究[J]. 河南农业科学, 2023, 52(9): 78-90.
[8]	赵海军, 史佳晴, 王彬, 郭益洋, 胡小丽, 韩赞平. 150 份玉米自交系籽粒及其品质性状的综合评价[J]. 河南农业科学, 2023, 52(5): 33-39.
[9]	贺占雪, 朱太富, 李欣, 苏效兰, 刘惠民, 王连春. 不同砧穗组合对猕猴桃溃疡病的抗性差异及机制分析[J]. 河南农业科学, 2023, 52(1): 95-107.
[10]	刘芹, 特日根, 师子文, 吴杰, 胡素娟, 崔筱, 孔维丽. 不同品种香菇子实体游离氨基酸组成的主成分分析及综合评价[J]. 河南农业科学, 2022, 51(7): 134-144.
[11]	秦溱, 李金龙, 周先文, 戴华林, 王晓清, 谈晨. 湘西呆鲤形态性状与体质量的相关性分析及曲线拟合[J]. 河南农业科学, 2022, 51(6): 144-153.
[12]	冯链, 田翔, 乔治军, 王海岗. 谷子农艺性状与蛋白质含量相关性分析[J]. 河南农业科学, 2022, 51(6): 43-53.
[13]	王雪萍, 高士伟, 叶飞, 滕靖, 桂安辉, 郑鹏程. 茶树种苗质量评价与壮苗指数构建[J]. 河南农业科学, 2022, 51(12): 45-52.
[14]	李培艳, 谭元生, 韦金兑, 罗杰灵, 华国洪, 李华, 甘建伉. 清远麻鸡母系体尺性状与产蛋数的相关分析[J]. 河南农业科学, 2021, 50(9): 143-148.
[15]	石桃雄, 郑俊青, 黎瑞源, 吕丹, 陈庆富, 梁成刚. 苦荞种质资源种子蛋白质及组分含量的变异分析[J]. 河南农业科学, 2021, 50(9): 25-35.

外源硒添加对香菇金属元素累积与食用安全性的影响

Effects of Exogenous Selenium Addition on Metal Element Accumulation and Edible Safety of Lentinula edodes

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献

相关文章 15

编辑推荐

Metrics