关键词: 长豇豆, SSR, InDel, 遗传多样性, 聚类分析

Abstract: To explore the genetic background and richness of genetic diversity of local cowpea germplasm resources in Henan Province，genetic diversity analysis was conducted on 58 local cowpea germplasm resources using 55 pairs of molecular markers.The results showed that 319 loci were amplified using 55 pairs of molecular markers，including 310 polymorphic loci，with a polymorphism rate of 97.2%.The mean number of observed alleles（Na）was 2.018 2；the mean number of effective alleles（Ne）was 1.763 8；the mean Nei’s gene diversity index（H）was 0.418 1；the mean of Shannon index（I）was 0.608 1；the polymorphic information content（PIC）value was 0. 327 8.The results indicated that the selected primers had good polymorphism and there was good genetic diversity among the local cowpea germplasm resources in Henan Province. Cluster analysis categorized the 58 local long cowpea germplasm resources from Henan Province into population 1 and population 2. All germplasm resources in population 1 originated from the high‑altitude regions of western Henan，while those in Cluster 2 were primarily distributed in low‑altitude areas such as the Nanyang Basin and North China Plain. Molecular markers successfully separated all 58 germplasm resources，but the genetic differentiation between regions was not significant，indicating that local varieties in Henan Province had little relationship with their source regions，and the genetic variation of materials was less affected by local environments.

Key words: Long cowpea, SSR, InDel, Genetic diversity, Cluster analysis