河南农业科学 ›› 2021, Vol. 50 ›› Issue (11): 36-46.DOI: 10.15933/j.cnki.1004-3268.2021.11.005

• 作物栽培·遗传育种 • 上一篇    下一篇

采用立方体取样法提高小麦根系研究精准性的探讨

杨一晨1,杨习文1,吴寅2,黄源1,徐利利1,付锦州1,郭芳芳1,周苏玫1,贺德先1   

  1. (1.河南农业大学农学院/国家小麦工程技术研究中心/省部共建小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心,河南郑州450046;2.河南省农业科学院,河南郑州450002)
  • 收稿日期:2021-04-12 出版日期:2021-11-15 发布日期:2022-01-17
  • 通讯作者: 周苏玫(1965-),女,河南渑池人,教授,博士,主要从事小麦生态生理研究。E-mail:smzhou129@163.com 贺德先(1963-),男,河南南召人,教授,博士,主要从事小麦生态生理研究。E-mail:hed@henau.edu.cn
  • 作者简介:杨一晨(1995-),女,河南宝丰人,在读硕士研究生,研究方向:小麦栽培生理。E-mail:408995938@qq.com
  • 基金资助:
    国家重点研发计划项目(2018YFD0300701);河南省重点研发与推广专项(192102110142)

Discussion on Improving Precision of Wheat Root Research by Cube Sampling Method

YANG Yichen1,YANG Xiwen1,WU Yin2,HUANG Yuan1,XU Lili1,FU Jinzhou1,GUO Fangfang1,ZHOU Sumei1,HE Dexian1   

  1. (1.College of Agronomy,Henan Agricultural University/National Engineering Research Center for Wheat/Co‑construction State Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops,Zhengzhou 450046,China;2.Henan Academy of Agricultural Sciences,Zhengzhou 450002,China)
  • Received:2021-04-12 Published:2021-11-15 Online:2022-01-17

摘要: 为减少取样误差,提高小麦根系研究的精准性,于2018—2020年分别采用立方体取样法(CSM)、根钻法(CK1)、挖掘法(CK2)对西农979、周麦27进行不同生育时期不同土层根系生长发育和活性的比较研究。结果表明,相较于CK1和CK2处理,CSM处理的根干质量、根长、根表面积和根体积测定值较高,20~40 cm土层中不同处理上述指标测定值的差异大于0~20 cm土层,且在生育后期不同取样方法上述指标测定值之间差异较大。相较于CK1和CK2处理,CSM处理的根系活性测定值较高,通过不同取样方法测定的根系活性差异较大,尤其是在生育前期,而生育后期差异缩小;全生育期不同土层中根系活性的差异较大,表现为0~20 cm土层高于20~40 cm土层,而20~40 cm土层中不同取样方法下根系活性测定值的差异大于0~20 cm土层。相关性和回归分析结果表明,不同土层中不同取样方法根系性状测定值间呈极显著正相关;通过回归方程的建立,可根据CK1、CK2处理的根系性状测定值估算CSM处理根系性状测定值,从而对CK1、CK2的测定值进行矫正。比较而言,立方体取样法虽然操作要求高,但获取的根样代表性强,根系性状调查分析精准度高、误差小。

关键词: 小麦, 根系, 研究精准性, 立方体取样法, 根钻法, 挖掘法

Abstract: In order to reduce sampling error and improve accuracy of wheat root research,root growth and vigor in different soil layers during different growth stages were comparatively studied among cube sampling method(CSM),auger sampling method(CK1)and digging method with a spade(CK2)with two types of wheat cultivars Xinong 979 and Zhoumai 27 as materials in 2018—2020. The results showed that dry root weight,root length,root surface area and root volume of CSM treatment were higher at different growth stages compared with CK1 and CK2 treatments,the differences of root traits mentioned above in different treatments were higher in 20—40 cm soil layer than those in 0—20 cm soil layer,and thedifferences of root traits mentioned above in different treatments were higher in the late growth period.Root vigor of CSM treatment was higher than those of CK1 and CK2 treatments,the differences in root vigor among different treatments were bigger in the early growth period,and smaller in the late growth period.There was a big difference of root vigor in different soil layers during the growth period,root vigor was higher in 0—20 cm soil layer than that in 20—40 cm soil layer,and the difference of root vigor in 20—40 cm soil layer was bigger than that in 0—20 cm soil layer among different sampling methods.Correlation and regression analysis indicated that there was an extremely significant positive correlation among different sampling methods. Through the establishment of regression equation,the root character values of CSM treatment could be estimated according to the root character values of CK1 and CK2 treatments,so as to correct the values of CK1 and CK2 treatments.Comparatively speaking,root samples obtained by cube sampling method had strong representativeness,and hence led to more accurate root trait value,though cube sampling method was more laborious to operate.

Key words: Wheat(Triticum aestivum L.), Root system, Research accuracy

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