利用EMS诱变创制抗除草剂粳稻新种质

doi:10.15933/j.cnki.1004-3268.2021.04.002

河南农业科学 ›› 2021, Vol. 50 ›› Issue (4): 8-16.DOI: 10.15933/j.cnki.1004-3268.2021.04.002

利用EMS诱变创制抗除草剂粳稻新种质

王付华¹，李自超²，王亚¹，付景¹，杨文博¹，尹海庆¹，王生轩¹，王越涛¹，白涛¹，张珍³

（1.河南省农业科学院粮食作物研究所，河南郑州 450002; 2.中国农业大学农学院，北京 100193;3.河南省农业科学技术展览馆，河南郑州 450000）

收稿日期:2021-01-18 出版日期:2021-04-15 发布日期:2021-04-15
通讯作者: 尹海庆（1965-），男，河南南阳人，研究员，主要从事水稻分子育种工作。 E-mail：yinhq98@163.com
作者简介:王付华（1966-），男，湖南祁阳人，副研究员，博士，主要从事水稻分子育种工作。E-mail：wangfuhuahunan@163.com
基金资助:
国家重点研发计划项目(2017YFD0100400，2016YFD0100101)

New Herbicide-Resistant Japonica Rice Germplasms Created by EMS Mutagenesis

WANG Fuhua¹,LI Zichao²,WANG Ya¹,FU Jing¹,YANG Wenbo¹,YIN Haiqing¹,WANG Shengxuan¹,WANG Yuetao¹,BAI Tao¹,ZHANG Zhen³

(1.Institute of Cereal Crops,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Agricultural College,China Agricultural University,Beijing 100193,China;3.Henan Agricultural Science and Technology Exhibition Hall,Zhengzhou 450000,China)

Received:2021-01-18 Published:2021-04-15 Online:2021-04-15

摘要/Abstract

摘要： 为了创制抗除草剂水稻新种质，用EMS(甲基磺酸乙酯)溶液浸泡郑稻19干种子，在M2幼苗三叶期分别喷施112.5 g/hm²的甲咪唑烟酸和60 g/hm²的烟嘧磺隆，14 d后筛选抗除草剂突变体，并对突变体ALS(Acetolactate synthase)基因序列进行分析，找出ALS基因突变位点。结果表明，喷施甲咪唑烟酸14 d后，抗除草剂植株生长正常、叶色偏绿，敏感植株黄化、生长受抑制；喷施烟嘧磺隆14 d后，抗除草剂植株表现前期受抑制，但能缓慢恢复。用甲咪唑烟酸筛选63 000份M₂家系，获得抗除草剂突变体6份（HF1—HF6），包括2种ALS氨基酸突变类型：Ser-627-Asn和Gly-628-Glu。用烟嘧磺隆筛选M2家系30 000份，获得抗除草剂突变体2份（HF7—HF8），包括2种ALS氨基酸突变类型：Pro-171-Ser和Ala-179-Val。在M₂筛选到1个特定ALS碱基突变平均需23 250份M₂家系。筛选到的抗甲咪唑烟酸突变体HF1（Gly-628-Glu）和HF2（Ser-627-Asn）在1倍大田常规除草剂剂量（112.5 g/hm²）下鲜质量减退率分别为13.09%和20.03%，显著小于郑稻19（P<0.05）；在8倍大田常规除草剂剂量（900 g/hm²）下突变体仍能正常生长，表现强的甲咪唑烟酸抗性。对HF1、HF2分别喷施30 g/hm²和60 g/hm²烟嘧磺隆，其鲜质量减退率分别为24.81%、13.13%和34.13%、28.01%，显著小于郑稻19（P<0.05），表现一定的烟嘧磺隆抗性。综上，筛选到8份抗除草剂突变体材料，2份抗烟嘧磺隆，6份抗甲咪唑烟酸，其中2份抗甲咪唑烟酸突变体兼具烟嘧磺隆抗性，创制的抗除草剂材料可用于选育非转基因抗除草剂品种，也可作为创制ALS多位点突变种质的基础材料，进一步增强突变体对除草剂的抗性。

关键词: 粳稻；抗烟嘧磺隆；抗甲咪唑烟酸, 抗除草剂； EMS诱变；种质创建

Abstract: In order to create new herbicide-resistant rice germplasms,the dry seeds of Zhengdao 19 were soaked in ethylmethane sulfonate( EMS) solution.At three-leaf stage,M₂ seedlings were sprayed with imazapic(112.5 g/ha)and nicosulfuron(60 g/ha).The herbicide-resistant mutants were screened after 14 days.The ALS (acetolactate synthase) gene sequences of mutants were analyzed to find out the mutation sites.The results showed that the herbicide-resistant plants grew normally after spraying imazapic,the leaf kept green,while the leaves of sensitive lines yellowed,and their growth were inhibited;after spraying nicosulfuron,the growth of herbicide-resistant plants were inhibited in the early stage,but could recover slowly.A total of 63 000 M₂lines were screened with imazapic,and 6 imazapic-resistant mutants (HF1—HF6) were obtained,including two types of ALS mutations,Ser-627-Asn and Gly-628-Glu.A total of 30 000 M₂ lines were screened with nicosulfuron, and 2 herbicide-resistant mutants(HF7—HF8) were obtained,including two types of ALS mutations, Pro-171-Ser and Ala-179-Val. To
obtain a specific ALS nucleotide mutation,an average of 23 250 M₂ lines were needed to screen.The fresh weight reduction rates of imazapic-resistant mutants HF1 ( Gly-628-Glu) and HF2 ( Ser-627-Asn) were 13.09% and 20.03% respectively after spraying 1 time the conventional herbicide dose(112. 5 g/ha),which were significantly less than that of Zhengdao 19 (P<0.05); the mutants could still grow normally after spraying 8 times the conventional herbicide dose in the field,showing strong resistance to imazapic herbicides.The fresh weight reduction rates of mutants HF1 and HF2 were 24.81% and 13.13% after spraying 30 g/ha nicosulfuron,and were 34.13% and 28.01% after spraying 60 g/ha nicosulfuron,respectively,which were significantly less than those of Zhengdao 19 (P<0.05), showing certain nicosulfuron resistance. In summary, 8 herbicide-resistant mutant lines were obtained, 2 lines were resistant to nicosulfuron,6 lines were resistant to imazapic,and two of the imazapic-resistant mutants exhibited nicotinsulfuron resistance.The screened herbicide-resistant mutants could be used to breed non-GMO herbicide-resistant varieties,and also be used as a basic germplasm for the multi-site mutation of ALS,so as to further strengthen the herbicide resistance of mutants.

Key words: Japonica rice, Resistance to nicosulfuron, Resistance to imazapic, Resistance to herbicide;EMS mutagenesis, Germplasm innovation

中图分类号:

S511

王付华, 李自超, 王亚, 付景, 杨文博, 尹海庆, 王生轩, 王越涛, 白涛, 张珍. 利用EMS诱变创制抗除草剂粳稻新种质[J]. 河南农业科学, 2021, 50(4): 8-16.

WANG Fuhua, LI Zichao, WANG Ya, FU Jing, YANG Wenbo, YIN Haiqing, WANG Shengxuan, WANG Yuetao, BAI Tao, ZHANG Zhen. New Herbicide-Resistant Japonica Rice Germplasms Created by EMS Mutagenesis[J]. Journal of Henan Agricultural Sciences, 2021, 50(4): 8-16.

[1]	樊一凡, 张艳艳, 王艺媚, 李俊周, 杜彦修, 孙红正, 彭廷, 赵全志, 张静. 不同播期温光条件对籼稻产量和品质的影响[J]. 河南农业科学, 2024, 53(2): 17-27.
[2]	杜雪丽, 柯璐瑶, 柳展, 代明珠, 周宇, 李剑兴, 徐玖亮. 叶面喷施镁对紫米产质量、花青素及代谢物含量的影响[J]. 河南农业科学, 2024, 53(2): 28-36.
[3]	王付华, 李锐, 张骐飞, 马梦娟, 付景, 杨文博, 王生轩, 王越涛, 白涛, 尹海庆, 王亚. 耐直播粳稻种质资源筛选及其适宜种植密度研究[J]. 河南农业科学, 2023, 52(12): 31-41.
[4]	马学虎, 马昀君, 黄璜, 陈灿, 王忍, 张印, 伍世豪, 张泉, 张琴. 稻禽共生对水稻群体生长特性和产量形成的影响[J]. 河南农业科学, 2023, 52(11): 42-48.
[5]	张凯, 陈明睿, 刘秋员. 减氮增密对籼粳杂交稻产量和稻米品质的影响[J]. 河南农业科学, 2023, 52(10): 14-21.
[6]	赵彪, 冯旭, 贺付蒙, 徐永清, 石齐海, 冯艳忠, 李凤兰. EM富硒酵母菌剂的制备及其在水稻富硒生产中的应用[J]. 河南农业科学, 2023, 52(8): 26-35.
[7]	解文孝, 姜秀英, 吕军, 潘争艳, 韩勇, 王庆新, 李建国. 不同叶位功能叶片对不同穗型水稻籽粒灌浆特性及稻米品质的影响[J]. 河南农业科学, 2023, 52(8): 36-44.
[8]	豆丹丹, 孙建军, 王德新, 郭玉玺, 郭新海, 丁超明. 播期对早中稻产量和品质的影响[J]. 河南农业科学, 2023, 52(7): 12-23.
[9]	雷振山, 李猛, 卫云飞, 刘秋员, 刘娟, 王付娟, 季新. 氮肥运筹对豫南地区优质食味粳稻产量和品质的影响[J]. 河南农业科学, 2023, 52(6): 12-21.
[10]	黄海, 瞿小杰, 刘金海, 彭赞文. 盐碱胁迫下不同倍性水稻4 种转录因子家族基因的表达模式分析[J]. 河南农业科学, 2023, 52(6): 22-33.
[11]	张秀锦, 张容慧, 蔡景行, 王国坤, 柴冠群, 黄承玲, 范成五. 镍污染稻田低积累水稻品种筛选及健康风险评价[J]. 河南农业科学, 2023, 52(6): 61-69.
[12]	彭成林, 佀国涵, 赵书军, 袁家富, 吕洪九, 徐祥玉, 徐大兵, 谢媛圆, 周剑雄. 长期稻虾共作模式下氮肥运筹对直播水稻养分吸收和产量的影响[J]. 河南农业科学, 2023, 52(5): 9-16.
[13]	熊瑞, 张玮, 钟康裕, 周文涛, 欧茜, 王泓睿, 龙攀, 徐莹, 傅志强. 秸秆还田与轮耕对双季稻土壤物理特性及碳氮含量的影响[J]. 河南农业科学, 2023, 52(5): 81-90.
[14]	雷振山, 李猛, 卫云飞, 季新, 刘娟, 王付娟, 刘秋员. 不同种植方式对豫南地区优质食味粳稻产量及品质的影响[J]. 河南农业科学, 2023, 52(2): 12-20.
[15]	汤伟, 陈灿, 黄璜, 周天送, 王忍. 不同作物+小龙虾种养模式作物产量及氮、磷平衡分析[J]. 河南农业科学, 2023, 52(1): 24-33.

利用EMS诱变创制抗除草剂粳稻新种质

New Herbicide-Resistant Japonica Rice Germplasms Created by EMS Mutagenesis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics