关键词: 鸡白痢沙门氏菌, 植物乳杆菌, 益生性能, 抑菌性能, 耐受性, 黏附性, 安全性, 传代稳定性

Abstract: The aim of this study was to investigate the biological characteristics of a strain of Lactobacillus plantarum with strong ability to antagonize Salmonella, and to evaluate the feasibility and safety of Lactobacillus plantarum as feed probiotics.The strains were screened by Oxford cup antimicrobial test,and the strain with the strongest antibacterial property was co-cultured with Salmonella to determine its antagonistic ability to Salmonella.Then the growth performance, tolerance to adverse environment,adhesion to intestinal mucosa, safety and passage stability were studied.The results showed that Lactobacillus plantarum BLCC2-0410 had the best inhibitory effect on Salmonella pullorum.Lactobacillus plantarum reduced the growth of Salmonella pullorum by approximately 3 logs after co-culture for 24 h compared to the control.The growth of Lactobacillus plantarum entered logarithmic growth phase at 2 h after inoculation,and the highest viable count was 4.7×10⁹ cfu/mL.The minimum pH value of the culture medium was 3.74.It was tolerant to pH values of 2.5 and 3.0,and had certain tolerance to 0.1%—0.3% avian bile salt environment.The survival rate was 34. 3% in pepsin solution and 77.8% in trypsin solution after 4 h,and the adhesion rate to intestinal mucosa was 18.2%.The security test results showed that feeding Lactobacillus plantarum BLCC2-0410 did not affect the feed intake of chicks and there were no visible lesions in the lung,spleen,liver,kidney,etc..The strain of Lactobacillus plantarum BLCC2-0410 was continuously passed for 5,15,25 generations,and there was no significant difference in the viable count,bacterial morphology,pH value of fermentation broth and the diameter of inhibition zone to Salmonella pullorum.In conclusion, the strain of Lactobacillus plantarum BLCC2-0410 has obvious antimicrobial properties,excellent growth characteristics,strong acid production capacity,strong tolerance
to acids,bile salts and digestive enzymes,certain adhesion ability,no side effects on chickens and good passage stability,which can be used as a potential probiotic for further study.

Key words: Salmonella pullorum, Lactobacillus plantarum, Probiotic properties, Antimicrobial properties, Tolerance, Adhesion, Security, Passage stability