关键词: 蛹虫草, 铜离子耐受性, 菌丝体生长, 子实体生长, 多糖含量, 富集特征

Abstract: To study the effects of Cu²⁺ enrichment on growth of Cordyceps militaris and human health,different concentration of Cu²⁺was added into culture medium to observe the effects of Cu²⁺on the growth of mycelia and fruiting bodies of C.militaris,the content of polysaccharides and Cu²⁺enrichment.The results showed that Cu²⁺inhibited the growth of C.militaris,because the addition of Cu²⁺at the minimum concentration(1.6 mg/L) led to degenerative changes in the fruiting bodies of C.militaris,such as dwarfing and bifurcation.The polysaccharide content of C.militaris increased with the increase of Cu²⁺addition. When the Cu²⁺addition was 3.2 mg/L,the Cu²⁺enrichment rate of mycelium and fruiting body was the highest,54.41% and 191.01% respectively.Therefore,by comparing the above factors,it is found that the optimum Cu²⁺addition is 1.6—3.2 mg/L.With the minimum copper ion addition of 1.6 mg/L, 29 g dry or 116 g fresh fruiting bodies are needed daily to supplement the amount of Cu²⁺needed for people.

Key words: Cordyceps militaris, Cu²⁺tolerance, Growth of mycelia, Growth of fruiting body, Content of polysaccharides, Enrichment characteristic