樱桃谷鸭IP3R3基因多态性与蛋壳品质的关联分析

doi:10.15933/j.cnki.1004-3268.2021.06.017

河南农业科学 ›› 2021, Vol. 50 ›› Issue (6): 142-148.DOI: 10.15933/j.cnki.1004-3268.2021.06.017

樱桃谷鸭IP3R3基因多态性与蛋壳品质的关联分析

廖朝美^1,2,3，李杰章^1,2,3，游敏芳^1,2,3，谭光辉^1,2,3，张依裕^1,2,3

（1.贵州大学高原山地动物遗传育种与繁殖教育部重点实验室，贵州贵阳 550025；2.贵州大学贵州省动物遗传育种与繁殖重点实验室，贵州贵阳 550025；3.贵州大学动物科学学院，贵州贵阳 550025）

收稿日期:2021-01-03 出版日期:2021-06-15 发布日期:2021-06-15
通讯作者: 张依裕（1976-），男，贵州毕节人，教授，博士，主要从事动物遗传资源保护与利用研究。E-mail:zyy8yyc@163.com
作者简介:廖朝美（1996-），女，贵州黄平人，在读硕士研究生，研究方向：动物遗传育种与繁殖。E-mail:3285438093@qq.com
基金资助:
国家自然科学基金项目（31760663）；贵州省科技人才补助项目(黔科合平台人才［2017］5788)；贵州省千层次创新型人才项目（701030174401）

Association Analysis between Polymorphism of IP3R3 Gene and Eggshell Quality in Cherry Valley Duck

LIAO Chaomei^1,2,3,LI Jiezhang^1,2,3,YOU Minfang^1,2,3,TAN Guanghui^1,2,3,ZHANG Yiyu^1,2,3

(1.Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,
Guizhou University,Guiyang 550025,China; 2.Key Laboratory of Animal Genetics,Breeding and Reproduction of Guizhou
Province,Guizhou University,Guiyang 550025,China; 3.College of Animal Science,Guizhou University,Guiyang 550025,China)

Received:2021-01-03 Published:2021-06-15 Online:2021-06-15

摘要/Abstract

摘要： 为了探究三磷酸肌醇受体3型（IP3R3）基因多态位点对蛋鸭蛋壳品质的影响，试验以樱桃谷鸭为材料，收集45周龄鸭蛋测定蛋质量、蛋形指数、蛋壳厚度、蛋壳强度和蛋壳质量5个指标，并与突变位点进行关联性分析。结果显示，在IP3R3基因中检测到2个SNP位点，分别为第6外显子的g.5539186 T>C突变和第8外显子的g.5540404 G>A突变，2个SNP位点均为同义突变，均存在3种基因型；2个SNP位点均为中度多态，均未偏离Hardy-Weinberg平衡（P>0.05），2个SNP位点之间不存在强的连锁不平衡，共存在3种单倍型和5种双倍型。关联性分析结果显示，g.5539186 T>C位点对蛋壳品质的影响未达到显著水平（P>0.05）；g.5540404 G>A位点GG和GA基因型个体的蛋壳强度显著高于AA基因型个体（P<0.05），与其他蛋壳品质之间未达到显著关联；双倍型H1H1、H1H3、H2H3和H3H3个体的蛋壳厚度和蛋壳强度显著高于H2H2个体的蛋壳厚度和蛋壳强度（P<0.05），其他蛋壳品质不存在显著差异（P>0.05）。以上结果表明，g.5540404 G>A位点对蛋壳强度有显著影响，2个SNP位点组合基因型对蛋壳厚度和蛋壳强度也产生显著影响，g.5539186 T>C和g.5540404 G>A位点可作为鸭蛋蛋壳品质选择的遗传标记位点。

关键词: 三磷酸肌醇受体3型基因, 樱桃谷鸭, SNP位点, 蛋壳品质, 关联性分析

Abstract: In order to explore the effect of inositol trisphosphate receptor type 3 (IP3R3) gene polymorphism on eggshell quality of laying ducks, Cherry valley duck was used as material in the experiment,45-week-old duck eggs were collected to determine egg weight,egg shape index,eggshell thickness,eggshell strength and eggshell quality,and correlation between five indexes and mutation sites was analyzed.The results showed that two SNP loci were found in IP3R3 gene,respectively g.5539186 T>C mutation in exon 6 and g.5540404 G>A mutation in exon 8.The two SNP loci were synonymous mutations,and there were three genotypes. The two SNP loci were moderately polymorphic and did not deviate from Hardy-Weinberg equilibrium(P>0.05),there was no strong linkage disequilibrium between the two SNP loci,and there were three haplotypes and five diplotypes. The correlation analysis showed that the effect of g. 5539186 T>C locus on eggshell quality was not significant (P>0.05),the eggshell strength of GG and GA genotypes at g. 5540404 G >A locus was significantly higher than that of AA genotype (P<0.05), and there was no significant correlation with other eggshell quality.The shell thickness and strength of diplotypes H1H1,H1H3,H2H3 and H3H3 were significantly higher than those of H2H2 (P<0.05).There was no significant difference in other eggshell quality indexes(P>0.05).The above results show that the g.5540404 G>A locus has a significant effect on eggshell strength, and combined genotypes of two SNP loci also have a significant effect on eggshell thickness and eggshell strength.g.5539186 T>C and g.5540404 G>A loci can be used as genetic marker loci for duck eggshell quality selection.

Key words: IP3R3, Cherry valley duck, SNP locus, Eggshell quality, Correlation analysis

中图分类号:

S834.8

廖朝美, 李杰章, 游敏芳, 谭光辉, 张依裕. 樱桃谷鸭IP3R3基因多态性与蛋壳品质的关联分析[J]. 河南农业科学, 2021, 50(6): 142-148.

LIAO Chaomei, LI Jiezhang, YOU Minfang, TAN Guanghui, ZHANG Yiyu. Association Analysis between Polymorphism of IP3R3 Gene and Eggshell Quality in Cherry Valley Duck[J]. Journal of Henan Agricultural Sciences, 2021, 50(6): 142-148.

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樱桃谷鸭IP3R3基因多态性与蛋壳品质的关联分析

Association Analysis between Polymorphism of IP3R3 Gene and Eggshell Quality in Cherry Valley Duck

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