关键词: 猕猴桃, 贮藏, 分离, 病原菌, 抑菌中药, 蛇床子, 苦楝皮, 连翘, 致病性, 抑菌作用

Abstract: In order to clarify the types of common pathogens during kiwifruit storage and explore effective control measures,pathogens were isolated from kiwifruit during storage by plant tissue isolation method.Their taxonomic status were confirmed by morphological observation and ITS sequence analysis.The inhibition effect of PDA media containing common antifungal Chinese medicine on mycelial growth of pathogens were determined.The results of morphology and ITS sequence analysis showed that 5 pathogens were isolated,which were identified as Mucor irregularis(Rhizomucor variabilis),Alternaria brassicae,Botryosphaeria dothidea,Alternaria alternate and Diaporthe eres respectively.The results of antifungal Chinese medicine test showed that 0.3 g fructus cnidii,Cortex meliae and Forsythia in 1 mL PDA medium had better inhibition effect on common kiwifruit pathogens,and the inhibition rate of mycelial growth of five pathogenic fungi reached more than 70%,which was significantly higher than that of other single Chinese medicines. Antifungal Chinese medicine has good inhibition effects on common fungi during kiwifruit storage,and has potential application value in production.

Key words: Kiwifruit, Storage, Isolation, Pathogens, Antifungal Chinese medicine, Fructus cnidii, Cortex meliae, Forsythia, Pathogenicity, Inhibition effect