关键词: 人参黑斑病, 林下参, 内生拮抗真菌, 鉴定, 球毛壳菌, 发酵条件优化

Abstract: The antagonistic endophytic fungi were isolated and screened from healthy mountain-cultivated ginseng leaves,to provide good strains for the biological control of ginseng black disease.Tissue isolation method was used to isolate the endophytic fungi.The antagonistic fungi was identified based on morphological characteristics and phylogenetic analyses of 18S rDNA.The single factor experiment and orthogonal experiment were adopted to optimize the liquid fermentation medium and conditions.The results showed that endophytic fungi were isolated from healthy mountain-cultivated ginseng leaves.Among them,strain FS-01 was found to have strong antagonistic activity against Alternaria panax.Strain FS-01 was identified as Chaetomium globosum.The optimal medium was composed of 1.50% starch,1.00% yeast extract powder,0.05% dipotassium phosphate,0.10% magnesium sulfate and the optimal culture conditions were inoculation size of 6.0% and cultivated for 5 d at 30℃ with initial pH of 7.0.Strain FS-01 has strong antagonistic activity against Alternaria panax,and could be a biocontrol strain for developing against ginseng black disease.

Key words: Ginseng black disease;Mountain-cultivated ginseng;Antagonistic endophytic fungus, Identification;Chaetomium globosum, Fermentation condition optimization