Evolution and Expression Divergence of the Chitinase Gene Family against Leptosphaeria maculans and Sclerotinia sclerotiorum Infection in Brassica napus

doi:10.15933/j.cnki.1004-3268.2020.02.012

河南农业科学 ›› 2020, Vol. 49 ›› Issue (2): 89-103.DOI: 10.15933/j.cnki.1004-3268.2020.02.012

Evolution and Expression Divergence of the Chitinase Gene Family against Leptosphaeria maculans and Sclerotinia sclerotiorum Infection in Brassica napus

XU Wen¹,ZHOU Tengsheng²,LU Dandan¹,AN Sufang¹,HOU Jinna¹,LI Genyi^1,2

(1.Crop Designing Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Department of Plant Science,University of Manitoba,Winnipeg,MB R3T2N2,Canada)

收稿日期:2019-09-17 出版日期:2020-02-15 发布日期:2020-02-15
通讯作者: LI Genyi(1960-),male,Weishi County,Henan Province,professor,mainly engaged in rapeseed molecular breeding.E-mail:Genyi.Li@umanitoba.ca
作者简介:XU Wen(1986-),male,Xixian,Henan Province,Ph.D.,mainly engaged in bioinformatics and plant molecular breeding.E-mail:xuwenss@126.com
基金资助:
Independent Innovation Special Fund of Henan Academy of Agricultural Science (2018ZC78);Henan Fundamental and Frontier Research Fund(162300410153)

Evolution and Expression Divergence of the Chitinase Gene Family against Leptosphaeria maculans and Sclerotinia sclerotiorum Infection in Brassica napus

XU Wen¹,ZHOU Tengsheng²,LU Dandan¹,AN Sufang¹,HOU Jinna¹,LI Genyi^1,2

(1.Crop Designing Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Department of Plant Science,University of Manitoba,Winnipeg,MB R3T2N2,Canada)

Received:2019-09-17 Published:2020-02-15 Online:2020-02-15
About author:XU Wen(1986-),male,Xixian,Henan Province,Ph.D.,mainly engaged in bioinformatics and plant molecular breeding.E-mail:xuwenss@126.com

摘要/Abstract

摘要： 黑胫病和菌核病是造成世界油菜产量下降的2种主要病害，分别由油菜茎基溃疡病菌和核盘菌引起。几丁质酶基因家族被认为是一类病害相关蛋白质，在植物抵抗病菌和非生物胁迫应答过程中发挥着重要作用。然而，甘蓝型油菜中关于几丁质酶基因家族进化及其应答黑胫病和菌核病的表达研究尚未有报道。本研究在甘蓝型油菜基因组中共鉴定了68个几丁质酶基因，分为5个不同的类，分布在15条染色体上。进化分析表明，甘蓝型油菜中几丁质酶基因家族成员的扩张主要是由片段复制和串联重复所致。此外，利用转录组测序数据分析了甘蓝型油菜几丁质酶基因家族应答2种病菌胁迫的表达谱。结果表明，在2种病菌侵染下，几丁质酶基因家族中有一些成员的表达均被诱导，也存在一些几丁质酶基因家族成员被特异地诱导表达。由此可见，甘蓝型油菜中几丁质酶基因家族不同成员的功能在长期进化过程中发生了分化，在应答不同病害胁迫中发挥特异的作用。

关键词: 甘蓝型油菜, 几丁质酶基因家族, 表达模式, 油菜茎基溃疡病菌, 核盘菌

Abstract: Blackleg and sclerotinia stem rot caused by Leptosphaeria maculans and Sclerotinia sclerotiorum respectively are two major diseases in rapeseed worldwide,which cause serious yield losses. Chitinases are pathogenesis-related proteins and play important roles in host resistance to various pathogens and abioticstress responses.However,a systematic investigation of the chitinase gene family and its expression profile against L.maculans and S.sclerotiorum infection in rapeseed remains elusive. In this study,68 chitinase genes were identified in Brassica napus genome.These genes were divided into five different classes and distributed among 15 chromosomes.Evolutionary analysis indicated that the expansion of the chitinase gene family was mainly attributed to segmental and tandem duplication.Moreover,the expression profile of the chitinase gene family was investigated using RNA sequencing (RNA-Seq) and the results revealed that some chitinase genes were both induced while the other members exhibited distinct expression in response to L.maculans and S.sclerotiorum infection.Thus,the functions of different chitinase gene family members have diverged during long-term evolution and might exhibit different roles against different disease stresses in Brassica napus.

Key words: Brassica napus, chitinase gene family, expression pattern, Leptosphaeria maculans, Sclerotinia sclerotiorum

中图分类号:

S565.4

XU Wen, ZHOU Tengsheng, LU Dandan, AN Sufang, HOU Jinna, LI Genyi. Evolution and Expression Divergence of the Chitinase Gene Family against Leptosphaeria maculans and Sclerotinia sclerotiorum Infection in Brassica napus[J]. 河南农业科学, 2020, 49(2): 89-103.

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Evolution and Expression Divergence of the Chitinase Gene Family against Leptosphaeria maculans and Sclerotinia sclerotiorum Infection in Brassica napus

Evolution and Expression Divergence of the Chitinase Gene Family against Leptosphaeria maculans and Sclerotinia sclerotiorum Infection in Brassica napus

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