磷酸二酯酶通过cAMP-PKA 途径调控核盘菌菌核原基的形成

doi:10.15933/j.cnki.1004-3268.2022.09.010

河南农业科学 ›› 2022, Vol. 51 ›› Issue (9): 99-104.DOI: 10.15933/j.cnki.1004-3268.2022.09.010

磷酸二酯酶通过cAMP-PKA 途径调控核盘菌菌核原基的形成

吕蕊花，陈萌，赵倩，冯昭，史琳娜，张喜荣

（陕西中医药大学医学技术学院，陕西咸阳 712046）

收稿日期:2022-04-08 出版日期:2022-09-15 发布日期:2022-11-17
作者简介:吕蕊花（1987-），女，陕西周至人，讲师，博士，主要从事植物病害及病理研究。E-mail：lvruihua1987@163.com
基金资助:
陕西省自然科学基础研究计划项目（2021JQ-725）；陕西中医药大学学科创新团队项目（2019-YS04）；陕西省教育厅一般专
项科研计划项目（21JK0595）

Phosphodiesterase Regulates the Formation of Sclerotia Primordium of Sclerotinia sclerotiorum through cAMP⁃PKA Pathway

LÜ Ruihua，CHEN Meng，ZHAO Qian，FENG Zhao，SHI Linna，ZHANG Xirong

（College of Medical Technology，Shaanxi University of Chinese Medicine，Xianyang 712046，China）

Received:2022-04-08 Published:2022-09-15 Online:2022-11-17

摘要/Abstract

摘要： 在PDA培养基中添加不同浓度的磷酸二酯酶（PDEs）抑制剂阿托品，检测其对核盘菌生长发育的影响，并采用qRT-PCR方法检测PDEs主效基因及cAMP-PKA通路中相关基因的表达情况，研究核盘菌PDEs在cAMP-PKA通路中的作用，为开发以PDEs为靶标的新型杀菌剂提供理论基础。结果表明，当阿托品添加浓度为4 mmol/L时，核盘菌菌丝生长速率和菌核形成均受到影响。从核盘菌基因库中检索出了8个PDEs，qRT-PCR结果显示，PDE1/2在核盘菌菌核形成过程中表达量较高。添加PDEs抑制剂后，cAMP-PKA通路中PDEs上游基因AC 和RAS 表达量上调，但PDE1、PDE2和下游因子PKA表达量均下降。由此可知，PDEs在核盘菌菌核形成方面具重要作用，PDEs抑制剂通过抑制PDE1/2的表达使得下游因子PKA表达量下降，进一步影响菌核原基的形成。

关键词: 核盘菌, 磷酸二酯酶, cAMP-PKA途径, 菌核形成

Abstract: To study the role of phosphodiesterase（PDEs）in the formation of sclerotia primordium of Sclerotinia sclerotiorum，and provide a theoretical basis for the development of new fungicides targeting PDEs，different concentrations of atropine，a PDEs inhibitor，were added to the PDA medium to detect its effects on the growth and development of Sclerotinia sclerotiorum，and qRT⁃PCR was used to detect the expression of major genes of PDEs and in the cAMP⁃PKA pathway.The results showed that the mycelial growth rate of Sclerotinia sclerotiorum and the formation of sclerotia were affected when the concentration
of atropine was 4 mmol/L. Eight PDEs were retrieved from the Sclerotinia sclerotiorum gene bank，and qRT⁃PCR results showed that PDE1/2 were highly expressed during the formation of sclerotia.After the addition of PDEs inhibitors，the expressions of the upstream genes of PDEs（AC and RAS） in the cAMP⁃PKA pathway were up⁃regulated，but the expressions of PDE1，PDE2 and the downstream factor PKA were all decreased. It can be seen that PDEs play an important role in the formation of sclerotia of Sclerotinia sclerotiorum.PDEs inhibitors reduce the expression of downstream factor PKA by inhibiting
the expression of PDE1/2，which further affects the formation of sclerotia primordium.

Key words: Sclerotinia sclerotiorum, Phosphodiesterase, cAMP?PKA pathway, Sclerotia formation

中图分类号:

S432.44

吕蕊花, 陈萌, 赵倩, 冯昭, 史琳娜, 张喜荣. 磷酸二酯酶通过cAMP-PKA 途径调控核盘菌菌核原基的形成[J]. 河南农业科学, 2022, 51(9): 99-104.

LÜ Ruihua, CHEN Meng, ZHAO Qian, FENG Zhao, SHI Linna, ZHANG Xirong. Phosphodiesterase Regulates the Formation of Sclerotia Primordium of Sclerotinia sclerotiorum through cAMP⁃PKA Pathway[J]. Journal of Henan Agricultural Sciences, 2022, 51(9): 99-104.

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磷酸二酯酶通过cAMP-PKA 途径调控核盘菌菌核原基的形成

Phosphodiesterase Regulates the Formation of Sclerotia Primordium of Sclerotinia sclerotiorum through cAMP⁃PKA Pathway

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