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For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Comprehensive Evaluation of Nutritional Quality of Foxtail Millet Based on Principal Component Analysis and Membership Function Method ZHANG Aiqin, GUO Bin, LIU Lilong, LI Xuhua, WANG Qing, XU Rui, ZHANG Huan Journal of Henan Agricultural Sciences    2025, 54 (1): 28-39.   DOI: 10.15933/j.cnki.1004-3268.2025.01.004 Abstract （1738）      PDF （4001KB）（157）       Knowledge map    Save In order to explore the characteristics of nutritional content of different foxtail millet varieties from Longzhong area in Gansu Province，establish a standard evaluation system of nutritional quality and screen the foxtail millet varieties with excellent nutritional quality，10 foxtail millet varieties from Longzhong area(Huining County，Baiyin City)in Gansu Province were used as materials，19 nutritional quality indexes including basic nutrients(moisture，ash，carbohydrate，protein，crude fat，starch，amylose，amylopectin contents and amylose/amylopectin)，functional nutrients(flavone，total phenol，crude polysaccharide contents)and mineral elements(Zn，Mn，Cu，Fe，Ca，Mg，Se contents)were measured，and principal component analysis and membership function methods were used to evaluate the nutritional quality of foxtail millet. The results showed that there was significant difference in the contents of 18 nutritional components excluding moisture content from 10 foxtail millet varieties，with variation coefficients ranging from 1.58% to 103.46%，which indicated that there was great difference in the individual nutritional quality index among different foxtail millet varieties. Protein，amylose，polysaccharide，Mg，Se and Zn contents were determined as core indexes for the evaluation of nutritional quality of foxtail millet by principal component analysis，correlation analysis and cluster analysis. The nutritional quality evaluation model was established by principal component analysis，and the top three foxtail millet varieties were M‑8(Longgu 23)，M‑10(Longgu 029)and M‑9(Longgu 032)based on the comprehensive score of nutritional quality，consistent with the results of the membership function evaluation，which indicated that these three varieties had better nutritional quality.Ten foxtail millet varieties could be divided into three categories by cluster analysis，and class Ⅱ included M‑8 and M‑9，whose comprehensive nutritional quality performed better. Table and Figures | Reference | Related Articles | Metrics Select Comprehensive Evaluation of Photosynthetic Performance of Foxtail Millet Varieties and Screening of High Photosynthetic Efficiency Varieties YAN Feng, DONG Yang, ZHAO Fuyang, HOU Xiaomin, LI Qingquan, WANG Lida, LI Qingchao, LIU Yang, ZHANG Lei, LIU Kai Journal of Henan Agricultural Sciences    2024, 53 (5): 40-48.   DOI: 10.15933/j.cnki.1004-3268.2024.05.005 Abstract （1496）      PDF （1323KB）（456）       Knowledge map    Save A field experiment was conducted to determine the photosynthetic indexes[intercellular CO2 concentration(X1),ranspiration rate(X2),stomatal conductance(X3),net photosynthetic rate(X4),instantaneous water use efficiency(X5)]and leaf morphological indexes［leaf area(X6)，dry leaf weight(X7),specific leaf weight(X8)]of 37 foxtail millet varieties,the fuzzy membership function,principal component analysis,correlation analysis and cluster analysis were used to comprehensively evaluate the photosynthetic performance of foxtail millet varieties,the varieties with high photosynthetic efficiency were screened,and the regression equation of photosynthetic performance prediction was established by stepwise regression analysis,so as to provide a reference for the screening and utilization of high photosynthetic efficiency foxtail millet germplasm. The results showed that the photosynthetic indexes and leaf morphological indexes were significantly different among 37 foxtail millet varieties.The CV ranged from 3.41% to 33.94%.The CV of stomatal conductance was the largest(33.94%),and the CV of specific leaf weight was the smallest(3.41%).The results of correlation analysis showed that intercellular CO2 concentration was significantly negatively correlated with transpiration rate and net photosynthetic rate;Transpiration rate was significantly positively correlated with net photosynthetic rate;Instantaneous water use efficiency was significantly negatively correlated with transpiration rate and net photosynthetic rate;Leaf area was significantly positively correlated with dry leaf weight.Principal component analysis was used to transform the 8 indicators into 3 independent comprehensive indicators.Transpiration rate and net photosynthetic rate could be used as evaluation indicators for the identification of photosynthetic performance of foxtail millet.Through cluster analysis,37 foxtail millet varieties were divided into three categories(high photosynthetic efficiency，medium photosynthetic efficiency and low photosynthetic efficiency),and 8 high photosynthetic efficiency varieties,including Nenxuan 22,Datong 25,Gonggu 88,Yangu 18,Jingu 20，Mengfeng 7,Longgu 13 and Gonggu 75,were screened out,which could be used as parent materials for high photosynthetic efficiency breeding.The regression model of foxtail millet photosynthetic performance was established by stepwise regression analysis(D=-1.391 984 196+0.000 619 787 003 4X1+0.051 519 289 48X2+0.674 399 672 2X3+0.013 844 082 338X4+0.058 230 284 78X5+0.003 403 878 541X6+0.051 881 997 92X8）. Reference | Related Articles | Metrics Select Effects of Phosphorus Fertilizer Application Depth on Root Distribution，Nitrogen Uptake and Utilization and Yield of Foxtail Millet ZHU Cancan, FU Senjie, QIN Na, WANG Chunyi, DAI Shutao, SONG Yinghui, WEI Xin, LI Junxia Journal of Henan Agricultural Sciences    2023, 52 (12): 22-30.   DOI: 10.15933/j.cnki.1004-3268.2023.12.003 Abstract （1611）      PDF （1455KB）（660）       Knowledge map    Save In order to provide a theoretical basis for the efficient utilization of phosphate fertilizer in foxtail millet production，a field experiment was conducted with four phosphate fertilizer application depths of 5 cm（P5），10 cm（P10），15 cm（P15）and 20 cm（P20）with no phosphate fertilizer application as control（CK），and the effects of phosphorus fertilizer application depth on root distribution，dry matter accumulation and transportation，nitrogen uptake and utilization，and yield of foxtail millet were studied.The results showed that suitable application depth of phosphate fertilizer promoted the growth of foxtail millet roots，and increased root length，root surface area and dry weight of root，showing a trend of P15>P10>P5>P20>CK. With the increase of phosphate fertilizer application depth，the root length，root surface area and dry weight of root of foxtail millet in deep soil increased，and the values showed P20>P15>P10>P5>CK in 20—40 cm soil layer. Suitable application depth of phosphate fertilizer could promote dry matter accumulation and transportation of foxtail millet. Compared with P5 treatment，the dry matter accumulations at maturity of P10，P15 and P20 treatments increased by 4.60%，10.29% and 0.92%，respectively，and the dry matter transfer rates increased by 6.49%，10.06% and -20.13%，respectively.Deep application of phosphate fertilizer increased the uptake and utilization of nitrogen by roots in deep soil.The available nitrogen content in deep soil，especially in the 20—40 cm soil layer，significantly decreased compared with P5 treatment. Compared with P5 treatment，the total nitrogen accumulations of P10，P15 and P20 treatments increased by 3.91%，17.85% and 5.31% at maturity，respectively；nitrogen absorption efficiencies increased by 5.88%，19.12% and 7.35%，and partial productivities of nitrogen increased by 18.95%，32.88% and -1.26%，respectively. Suitable application depth of phosphate fertilizer increased grain weight per spike，yield and harvest index，which all showed P15>P10>P5>P20>CK，the yields of P10 and P15 treatments increased by 18.98% and 32.86% compared with P5 treatment，respectively，and the yield of P20 treatment decreased by 1.27% compared with P5 treatment.In conclusion，15 cm is the optimal depth of phosphorus fertilizer application，which can promote the growth and down lapping of foxtail millet roots，improve the uptake and utilization of nitrogen by roots in soil，promote the accumulation and transportation of dry matter，and increase the yield by 32.86% compared with the shallow application of phosphorus fertilizer at 5 cm. Reference | Related Articles | Metrics Select Differences in Natural Enrichment Abilities of Elements in Root Tubers of Different Genotypes of Sweet Potato（Ipomoea batatas  Lam.） CHEN Han, HU Huiyun, CHENG Jianfeng, HUANG Yingjin, MAO Xiaorong, XU Xiaoming, ZENG Yanhua Journal of Henan Agricultural Sciences    2023, 52 (7): 24-39.   DOI: 10.15933/j.cnki.1004-3268.2023.07.003 Abstract （1259）      PDF （1286KB）（757）       Knowledge map    Save The contents of iron，zinc，selenium，magnesium，cadmium，chromium，lead，mercury and arsenic of root tubers in 16 different use types of sweet potato（Ipomoea batatas Lam.）were analyzed，and the natural enrichment ability of element in root tubers of different genotypes of sweet potato was comprehensively evaluated by fuzzy membership function method，EW⁃TOPSIS （entropy weight technique for order preference by similarity to ideal solution） and fuzzy comprehensive evaluation method，so as to clarify the difference of natural enrichment ability of element in root tuber of different genotypes of sweet potato，and define the sweet potato genotypes with high enrichment of beneficial elements and low enrichment of harmful elements，which would provide the theoretical basis and practical guidance for genetic improvement and planting of high⁃yield，high⁃quality，nutritional and safe sweet potato varieties.The results showed that there were great differences in yield of fresh root tubers among different sweet potato genotypes，and there were significant or extremely significant differences among fresh⁃eating or starch types，and the difference in yield of fresh root tubers among starch type was greater than that among fresh⁃eating type.There were significant or extremely significant differences in the content and accumulation of different beneficial and hazardous elements in the same fresh sweet potato genotypes or the same beneficial and hazardous elements in the different fresh sweet potato genotypes.Among the beneficial elements，the content and accumulation of magnesium were the highest，followed by iron，and selenium and zinc were lower；but the difference of selenium content and accumulation among genotypes was the largest，followed by iron，and zinc and magnesium were smaller.Among the hazardous elements，chromium content and accumulation were the highest，and the difference among genotypes was the largest，followed by cadmium and arsenic. There was a certain relationship between the use types of sweet potato and the content and accumulation of elements in their root tubers.The content and accumulation of magnesium and cadmium in fresh⁃eating type were generally higher than those in starch type，but those of iron，selenium and arsenic were generally lower，while zinc and chromium had basically no differences.There were significant differences in the natural enrichment abilities of elements（content and accumulation）in root tubers of different sweet potato genotypes，which were related to the evaluation index and weight.The comprehensive analysis indicated that the element enrichment ability in fresh root tubers of different sweet potato genotypes could be divided into high，medium and low categories.The two genotypes with high enrichment ability were Ganshu 203 and Gan K06，the four genotypes with medium enrichment ability were Pushu 32，Ganshu 3，Jishu 25 and Zhanshu 16，and the other ten genotypes had low enrichment ability. Reference | Related Articles | Metrics Select Comprehensive Evaluation of 176 Waxy Sorghum Germplasm Resources Based on Principal Component，Grey Correlation and DTOPSIS Analyses WU Guojiang, ZHOU Wei, YU Zhonghao, LI Yan, LÜ Jingbo, WANG Zhenguo, ZHOU Yaxing Journal of Henan Agricultural Sciences    2023, 52 (5): 40-51.   DOI: 10.15933/j.cnki.1004-3268.2023.05.006 Abstract （1564）      PDF （1325KB）（341）       Knowledge map    Save In order to screen out the high‐quality waxy sorghum germplasm resources suitable for popularization and utilization in the Xiliaohe Plain，16 main agronomic traits of 176 waxy sorghum germplasm resources were analyzed，and the comprehensive evaluation and ranking were carried out by combining principal component analysis method，grey correlation degree method and DTOPSIS method.The results showed that the coefficient of variation of the main agronomic traits of 176 waxy sorghum germplasm resources ranged from 6.69% to 47.84%，and there were generally significant or extremely significant correlation between the traits. Through principal component analysis，16 main agronomic traits of 176 waxy sorghum germplasm resources were integrated into 9 independent comprehensive indexes（principal components），and the cumulative contribution rate of these 9 principal components was87.169%.The top 30 germplasm resources were screened based on comprehensive evaluation scores by principal component analysis，weighted correlation degree by grey correlation degree method and relative closeness degree by DTOPSIS method respectively. By the combination of the above three methods for comprehensive evaluation，10 germplasm resources with excellent comprehensive traits were selected from 176 germplasm resources，namely QNR，9705，2011‐30，Xinliang 52，102A，HubocuoP4，Jan‐88，9701，0657 and 4126 ，respectively，which laid a foundation for the breeding of waxy sorghum varieties in the Xiliaohe Plain. Reference | Related Articles | Metrics Select Optimization of CRISPR/Cas9 Genome Editing Systems in Protoplasts of Setaria italica LIU Guangyu, XU Xiaojing, XIA Keke, SUN Haixi, TAO Yueru, CUI Zhen, GU Ying Journal of Henan Agricultural Sciences    2022, 51 (1): 34-42.   DOI: 10.15933/j.cnki.1004-3268.2022.01.005 Abstract （1767）      PDF （1847KB）（1389）       Knowledge map    Save To optimize and screen efficient genome editing systems in millet（Setaria italica），multiple CRISPR/Cas9（clustered regularly interspaced short palindromic repeats/CRISRP‐associated nuclease 9）genome editing systems were constructed with six kinds of gRNAs designed for phytoene desaturase（SiPDS） gene（gRNA1—gRNA2 for exon 1，gRNA3—gRNA6 for exon 12），and transformed into protoplasts of millet by polyethylene glycol‐mediated method. Then the mutation efficiency against SiPDS gene was rapidly detected by large‐scale parallel sequencing. The results showed that the protoplast transformation system established by the young stems of 7 day‐old millet etiolated seedlings had high transformation efficiency from 50. 44% to 57. 36%. Using this method，the gene editing systems with Cas9 gene driven by Super and Ubi promoters were transferred into millet protoplasts，the mutation efficiencies of SiPDS gene were 0. 5% and 5. 5% respectively. Thus，the Cas9 gene of gene editing systems with single gRNA，double gRNA or tRNA cassettes were driven by Ubi promoter，and mutation efficiency of SiPDS gene induced by these systems was compared. It was found that the mutation efficiencies of gene editing system with double gRNA cassettes such as Ubi‐dgRNAE1 and Ubi‐dgRNAE12 were 1. 66 and 1. 11 times higher than that with single gRNA system. The mutation efficiency induced by tRNA‐based system Ubi‐tRNA was 51. 24%，which was 5. 87 times higher than that with single gRNA system. Moreover，Ubi‐tRNA could simultaneously edit multiple sites of SiPDS gene，the multi‐site mutation frequency was 3. 23%. The cleavage activities in vitro of ribonucleoprotein（RNP）complexes which were mixture of gRNA3，gRNA4 or gRNA5 transcripts with Cas9 protein respectively were compared，it was found that RNP‐gRNA5 complex had the highest cleavage activity in vitro，the mutation efficiency of SiPDS gene was 2. 0%，and the dominant mutation type was deletion less than 3 bp. Table and Figures | Reference | Related Articles | Metrics Select Identification of Salt Tolerance Co-expression Modules and Hub Genes in Ipomoea batatas by WGCNA ZHANG Yi, WU Wanyi, LIU Xiayu, ZHANG Jie, TANG Ruimin, JIA Xiaoyun Journal of Henan Agricultural Sciences    2021, 50 (6): 16-27.   DOI: 10.15933/j.cnki.1004-3268.2021.06.003 Abstract （1731）      PDF （6924KB）（621）       Knowledge map    Save To explore the salt tolerance genes in Ipomoea batatas,we selected the top 50%(32 147)genes with large expression variation among different samples using transcriptome data of sweet potato roots at different time under salt stress,and conducted weighted gene co-expression network analysis(WGCNA).The results showed that 20 co-expressed modules were constructed,among which six gene modules were significantly associated with salt stress in sweet potato.Among the six gene modules related to salt stress,Green module and Red module were positively correlated with the salt stress at 48 h,Black and Yellow modules were positively correlated with the salt stress at 12 h,and Midnightblue and Magenta modules were negatively correlated with the salt stress at 48 h.Four specific modules which were positively correlated with salt stress were further analyzed.GO enrichment analysis showed that the four modules were significantly enriched in many items related to salt tolerance,such as transmembrane transport,cell response stimulated by salicylic acid,response to abiotic stimuli and response to hypertonic stress.By calculating the module relationships of genes within the module,bHLH115(basic helix-loop-helix 115), HAK5(high affinity K+transporter 5), NAGS2 (N-acetyl-L-glutamate synthase 2), DUF699 (GNAT acetyltransferase),TLP5(tubby like protein 5)and other genes were found to be the hub genes of these four modules,and the functional prediction suggested that these genes might play important roles in the resistance against stress.Through constructing interaction networks of hub genes,we found that FAR1(farred-impaired response 1),NAC[NAM(no apical meristem),ATAF1(Arabidopsis transcription activation factor 1),ATAF2,CUC2(cup-shaped cotyledon 2)] family gene NAC2,HsfB2a(heat shock transcription factor B2a) and other genes had high interaction with hub genes. This study provides a new idea for further study on the molecular mechanism of salt tolerance in sweet potato. Related Articles | Metrics