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Please wait a minute... For Selected: Download Citations EndNote Ris BibTeX Toggle Thumbnails Select Screening of miRNAs Related to High Temperature Stress in Maize Pollen and Analysis of Their Target Genes LI Chuan, ZHANG Panpan, ZHANG Meiwei, NIU Jun, MU Weilin, GUO Hanxiao, QIAO Jiangfang Journal of Henan Agricultural Sciences    2024, 53 (2): 1-16.   DOI: 10.15933/j.cnki.1004-3268.2024.02.001 Abstract （2848）      PDF （4835KB）（622）       Knowledge map    Save By using high⁃throughput miRNA sequencing technology，differentially expressed miRNAs were indentified in pollens of high heat tolerant maize variety Zhengdan 958 and low heat tolerant maize variety Xianyu 335 under high temperature stress at flowering stage，their target genes were predicted，and the enrichment analysis of genetic characteristics and metabolic pathways of these target genes were conducted. The results showed that a total of 818 miRNA precursor sequences were identified. Nineteen significantly differentially expressed miRNA sequences were screened in the group of Zhengdan 958 pollen under high temperature stress compared with the control pollen under normal condition(HT958 vs CK958). Among them，15 miRNA sequences were up⁃regulated，4 miRNA sequences were down⁃regulated，and expression level difference of 3 miRNA sequences reached extremely significant level(P<0.01).The predicted results of target genes of 19 differentially expressed miRNA sequences showed that a total of 503 gene transcripts were obtained，their enriched GO biological process terms included regulation of transcription，DNA⁃templated，microtubule⁃based process，hosphorylation，positive regulation of transcription by RNA polymerase Ⅱ ，methylation and so on，and the most significantly enriched KEGG pathways were glutathione metabolism，carbon metabolism，arachidonic acid metabolism lycolysis/gluconeogenesis，folate biosynthesis and so on. Fiveteen significantly differentially expressed miRNA sequences were identified in the group of Xianyu 335 pollen under high temperature stress compared with the control pollen(HT335 vs CK335). Among them，7 miRNA sequences were up⁃regulated,8 were down⁃regulated，and the expression level difference of 1 miRNA sequence reached highly significant level(P<0.01).The predicted results of target genes of 15 differentially expressed miRNA sequences showed that a total of 454 gene transcripts were obtained，their enriched GO biological process terms included regulation of transcription，DNA⁃templated，phosphorylation，protein phosphorylation,proteolysis，DNA repair and so on，and the most significantly enriched KEGG pathways were other glycan degradation,linoleic acid metabolism,metabolic pathways，thiamine metabolism，protein processing in endoplasmic reticulum and so on.Eighty⁃five significantly differentially expressed miRNA sequences were identified in the group of Zhengdan 958 pollen under high temperature stress compared with Xianyu 335 pollen under high temperature stress(HT985 vs HT335). Among them，35 miRNA sequences were up⁃regulated，50 were down⁃regulated，and expression level difference of 24 miRNA sequences reached extremely significant level(P<0.01). The predicted results of target genes of 85 differentially expressed miRNA sequences showed that a total of 2 286 gene transcripts were obtained，their enriched GO biological process terms included regulation of transcription，DNA⁃templated，phosphorylation,protein phosphorylation,proteolysis,transmembrane transport and so on，the most significantly enriched KEGG pathways were sphingolipidmetabolism，starch and sucrose metabolism，other glycan degradation，metabolic pathways，cysteine and methionine metabolism and so on. Ninety⁃four significantly differentially expressed miRNA sequences were identified in the HT958 vs CK958 group compared with HT335 vs CK335 group. The predicted results of target genes of 94 differentially expressed miRNA sequences showed that a total of 4 569 gene transcripts were obtained.Among them，the expression level difference of 28 miRNA sequences showed extremely significant(P<0.01),including PC⁃3p⁃10069_1143C⁃3p⁃18335_646，zma⁃miR164f⁃5p and so on. The enriched GO biological process terms of target genes of 94 differentially expressed miRNA sequences included regulation of transcription,DNA⁃templated,phosphorylation,protein phosphorylation,protein transport,proteolysis and so on,and the most significantly enriched KEGG pathways were protein processing in endoplasmic reticulum，ribosome biogenesis in eukaryotes，spliceosome，sphingolipid metabolism，endocytosis and so on. Reference | Related Articles | Metrics Select Expression and Functional Analysis of Maize Stress Response Gene ZmTPR1 CAO Liru, LIANG Xiaohan, MA Chenchen, YE Feiyu, PANG Yunyun, LI Weiya, ZHANG Xin, LU Xiaomin Journal of Henan Agricultural Sciences    2024, 53 (1): 12-21.   DOI: 10.15933/j.cnki.1004-3268.2024.01.002 Abstract （2338）      PDF （2292KB）（307）       Knowledge map    Save On the basis of previous transcriptome sequencing of maize under drought‑rehydration treatment，ZmTPR1 （tetratricopeptide repeat 1）was identified in response to drought stress.Bioinformatics analysis was conducted，and expression pattern was analyzed in different tissues and under different stresses. The homologous gene AtTPR1 in Arabidopsis thaliana was knocked out by CRISPR‑Cas9 technology，the phenotype，physiological and biochemical indexes of homozygous mutant plants were analyzed under drought stress，and the function of this gene was preliminarily explored，which would provide genetic resources for breeding drought‑resistant maize varieties.The results showed that ZmTPR1 gene was located on chromosome 3 of maize，encoding 421 amino acids，containing a conserved coiled coil domain，and might be involved in the response of maize to plant hormones，drought and so on.ZmTPR1 gene expressed in all tissues of maize，with the highest expression level in young stems.Drought，high temperature，salt and nitrogen deficiency stresses could induce the expression of ZmTPR1 gene，and the expression level of ZmTPR1 gene was most up‑regulated after drought stress.The expression level of ZmTPR1 gene in drought‑resistant maize inbred line Zheng 36 was significantly higher than that in drought‑sensitive maize inbred line B73 after drought stress. Drought resistance of Arabidopsis thaliana decreased after AtTPR1 gene was knocked out，the growth of Attpr1 mutant was severely inhibited under drought stress，and the leaves were wilted or even dried out. At the same time，the relative water content，chlorophyll content，net photosynthetic rate，activities of peroxidase and superoxide dismutase significantly decreased，and the content of malondialdehyde significantly increased.In summary，ZmTPR1 gene is involved in the response of maize to various abiotic stresses，and plays an important role in drought stress. Reference | Related Articles | Metrics Select Identification of Differentially Expressed bHLH Genes in Maize under Drought‑Rewatering Treatments XIE Xiaowen, ZHANG Xinyue, FU Jiaxu, SHAO Jing, WEN Pengfei, WANG Tongchao, WEI Li Journal of Henan Agricultural Sciences    2023, 52 (9): 33-44.   DOI: 10.15933/j.cnki.1004-3268.2023.09.004 Abstract （3403）      PDF （5798KB）（580）       Knowledge map    Save In order to explore bHLH transcription factors related to drought resistance in maize（Zea mays），differentially expressed bHLH genes were identified under drought‑rewatering treatment，and physicochemical characteristics，phylogenetic evolution，chromosome distribution，gene duplication，gene structure，conserved motif，cis‑elements in promoter region and gene expression were analyzed.The results showed that a total of 51 differentially expressed bHLH genes were identified in maize under drought‑rewatering treatment，the amino acid number，molecular weight and isoelectric point of 51 bHLH proteins ranged from 80 to 705 aa，21.26 to 92.17 ku，and 4.54 to 12.41，respectively.bHLH genes were divided into 16 subgroups，Ⅺ subgroup was the largest，containing 9 bHLH proteins；Ⅵ，Ⅷ，Ⅸ and ⅩⅢ subgroups were the smallest，containing 1 bHLH protein each. bHLH genes were distributed unevenly on 10 chromosomes，among which 7 pairs of genes had replication relationships.The number of exons varied greatly，9 bHLH genes contained 1 exon，27 bHLH genes contained 2—5 exons，and 15 bHLH genes contained 6 or more exons；Motif 1 and Motif 2 appeared more frequently in the conserved motifs of bHLH protein，followed by Motif 3 and Motif 5，with Motif 6 and Motif 9 appeared least frequently.The promoter region of bHLH genes contained many cis‑acting elements related to plant hormones and abiotic stress，such as ABRE，GARE‑motif，P‑box，AuxRR‑core，MBS，TGACG‑motif，CGTCA‑motif，TCA‑rich，TGA‑element and TCA‑element. Under drought‑rewatering treatment，51 bHLH genes exhibited different expression patterns，among them，14 genes such as ZmbHLH20，ZmbHLH25，ZmbHLH9，ZmbHLH137and ZmbHLH178 positively responded to drought stress，and 14 genes such as ZmbHLH58，ZmbHLH87，ZmbHLH36 and ZmbHLH106 negatively responded to drought stress，which were candidate genes for further study of drought response in maize bHLH family. Reference | Related Articles | Metrics Select Expression Patterns of Genes of Four Transcription Factor Families in Different Ploidy Rice under Salt⁃Alkali Stress HUANG Hai, QU Xiaojie, LIU Jinhai, PENG Zanwen Journal of Henan Agricultural Sciences    2023, 52 (6): 22-33.   DOI: 10.15933/j.cnki.1004-3268.2023.06.003 Abstract （1458）      PDF （1817KB）（1032）       Knowledge map    Save With diploid rice（Oryza sativa L.） Guiyu 12 and its autotetraploid rice as experimental materials，the changes of expression patterns of bZIP（basic leucine zippers），MYB（V⁃myb avian myeloblastosis viral oncogene homolog），AP2/ERF（APETALA2/ethylene response factor）and WRKY transcription factor family genes in diploid and tetraploid rice under saline⁃alkaline stress were studied，so as to lay the foundation for analyzing the saline⁃alkaline tolerance mechanism of different ploidy rice.The results showed that the same transcription factor gene in different ploidy rice had different responses to saline⁃alkali stress. Under salt stress，the induction（inhibition） degree of OsbZIP05，OsbZIP45，OsJAMYB and OsDREB1A（dehydration responsive element binding protein 1A）genes in tetraploid rice was higher than that in diploid rice.The expression trends of OsbZIP49，OsbZIP61，CSA（carbon starved anther），OsWRKY5，OsDREB41 and OsAP211 genes in tetraploid rice were completely opposite to that in diploid rice. Under alkaline stress，the induction（inhibition）degree of OsbZIP61，CSA，OsJAMYB and OsWRKY70 genes in tetraploid rice was higher than that in diploid rice.The expression trends of OsbZIP49，OsWRKY1，OsWRKY8，OsWRKY24，OsWRKY45，OsWRKY72，OsWRKY76，OsDREB1A and OsEREBP2（ethylene⁃responsive element binding protein 2） genes in tetraploid rice were completely opposite to that in diploid rice.In summary，the expression patterns of the same transcription factor gene in different ploidy rice under saline⁃alkali stress were significantly different，and the expression patterns of transcription factor gene in tetraploid rice induced by alkali stress were more complex. Reference | Related Articles | Metrics Select Identification and Analysis of WRKY Transcription Factors Differentially Expressed in Maize under Drought⁃Rewatering Treatment FU Jiaxu, YAN Yali, XIE Xiaowen, ZHANG Xinyue, WEN Pengfei, GUAN Xiaokang, WANG Tongchao, WEI Li Journal of Henan Agricultural Sciences    2022, 51 (8): 9-19.   DOI: 10.15933/j.cnki.1004-3268.2022.08.002 Abstract （3753）      PDF （4139KB）（869）       Knowledge map    Save In order to explore WRKY transcription factors related to drought resistance in maize（Zea mays），differentially expressed WRKY genes were identified under drought⁃rewatering treatment，and their physicochemical characteristics，phylogenetic evolution，chromosome distribution，gene duplication，gene structure，conserved motif，cis⁃elements in promoter region and gene expression level were analyzed.The results showed that a total of 51 differentially expressed ZmWRKY genes were identified，the amino acid number，molecular weight and isoelectric point of 51 ZmWRKY proteins ranged from 99 to 729 aa，11.22 to 78.73 ku，and 4.58 to 12.26，respectively.ZmWRKY genes were divided into three groups Ⅰ，Ⅱ and Ⅲ，ZmWRKY genes of group Ⅱ were divided into Ⅱa，Ⅱb，Ⅱc，Ⅱd and Ⅱe.ZmWRKY genes were unequally distributed on 10 chromosomes，including 2 pairs of tandem duplication and 16 pairs of segmental duplication.The ZmWRKY genes contained 1—12 exons，most of ZmWRKY proteins（41）contained 2—4 conserved motifs，and WRKY members in the same group had similar motif compositions.Many cis⁃elements related to plant hormone and abiotic stress，such as ABRE，AuxRR⁃core，TCA⁃element，TC⁃rich repeats，TGACG⁃motif，LTR，MBS，TATC⁃box，P⁃box，CGTCA⁃motif，GC⁃motif，TGA⁃element and GARE⁃motif were identified in ZmWRKY gene promotor region.Under drought⁃rewatering treatment，51 ZmWRKY genes exhibited different expression patterns，among them，fifteen genes positively responded to drought stress，including ZmWRKY1，ZmWRKY10，ZmWRKY16，ZmWRKY28， ZmWRKY30， ZmWRKY33， ZmWRKY42， ZmWRKY65， ZmWRKY68， ZmWRKY78，ZmWRKY96，ZmWRKY99，ZmWRKY100，ZmWRKY102 and ZmWRKY111，and one gene negatively responded to drought stress，which were candidate genes for further study of drought response in ZmWRKY family. 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