Evaluation and Analysis of the Main Phenotypic Traits of 86 Peanut Germplasm Resources

doi:10.15933/j.cnki.1004-3268.2024.10.008

Abstract

Abstract: In order to study the diversity of peanut germplasm resources and explore the application potential of excellent peanut germplasm resources,the coefficient of variation,Shannon diversity index,correlation analysis,principal component analysis and cluster analysis were used to identify 22 agronomic traits and analyze genetic diversity of 86 peanut germplasm resources.The results showed that the genetic diversity of peanut germplasm resources was rich,with the diversity index of 12 quality traits ranging from 0.15 to 1.08 and the coefficient of variation of 10 quantitative traits ranging from 1.71% to 29.25%,and there was genetic correlation among the phenotypic characters.Using Euclidean distance as genetic distance,86 peanut germplasm resources could be grouped into four groups.Among them group Ⅳ had the characteristics of strong seed dormancy，strong waterlogging resistance,short growth period，high pod yield,and rust resistance,etc.,which could be used as excellent parents of high⁃yield peanut.The cumulative contribution rate of the first eight principal components was 69. 382%.According to the information load in the principal component，growth period,100⁃pod weight,100⁃seed weight,kernel percent,main stem height,length of the 1st lateral branch could be used as the main evaluation index of peanut germplasm resources.The comprehensive score(F value)of peanut germplasm resources ranged from −1.05 to 0.89.Based on the sorting of comprehensive scores,five excellent peanut germplasm resource materials with F value>0.7 were selected,namely 201610,Puhua 82,201710,Fengshun 9616 and Jinonghua 33.

Key words: Peanut, Germplasm resources, Phenotypic identification, Genetic diversity, Comprehensive evaluation

摘要： 为探究花生种质资源的多样性，深度挖掘花生优异种质的应用潜力，采用变异系数、Shannon多样性指数、相关分析、主成分分析与聚类分析方法对86份花生种质资源的22个农艺性状进行鉴定及遗传多样性分析。结果表明，花生种质资源遗传多样性丰富，12个质量性状的多样性指数为0.15~1.08，10个数量性状的变异系数为1.71%~29.25%，各表型性状间具有遗传相关性。以欧氏距离为遗传距离进行聚类分析，可将86份花生种质资源聚为四大类群，其中第Ⅳ类群具有种子休眠性强、抗涝性强、生育期短、荚果产量高、抗锈病等特性，可作为高产花生的优良亲本。主成分分析提取前8个主成分，其成分累计贡献率达到69.382%，按照主成分中信息载量筛选出生育期、百果质量、百仁质量、出米率、主茎高、第一侧枝长可以作为花生种质资源评价的主要指标。花生种质资源的综合得分（F值）为-1.05~0.89，筛选出综合得分（F值）>0.7的优异花生种质资源材料5份，分别为201610、濮花82号、201710、丰顺9616、冀农花33号。

关键词: 花生, 种质资源, 表型鉴定, 遗传多样性, 综合评价

CLC Number:

S565.2

YU Mu, HU Yanling, YANG Haitang. Evaluation and Analysis of the Main Phenotypic Traits of 86 Peanut Germplasm Resources[J]. Journal of Henan Agricultural Sciences, 2024, 53(10): 63-72.

于沐, 胡延岭, 杨海棠. 86份花生种质资源主要表型性状的鉴评及分析[J]. 河南农业科学, 2024, 53(10): 63-72.

References

［1］廖伯寿.我国花生生产发展现状与潜力分析［J］.中国油料作物学报，2020，42（2）：161⁃166.
LIAO B S. A review on progress and prospects of peanut industry in China［J］. Chinese Journal of Oil Crop Sciences，2020，42（2）：161⁃166.
［2］张斌斌，蔡志翔，沈志军，等.观赏桃种质资源表型性状多样性评价［J］. 中国农业科学，2021，54（11）：2406⁃2420.
ZHANG B B，CAI Z X，SHEN Z J，et al.Diversity analysis of phenotypic characters in germplasm resources of
ornamental peaches［J］. Scientia Agricultura Sinica，2021，54（11）：2406⁃2420.
［3］王晓洁，黎美霞，陶蕾，等.48份黑果枸杞种质主要表型和品质性状的遗传多样性研究［J］. 河南农业科学，2023，52（9）：78⁃90.
WANG X J，LI M X，TAO L，et al.Study on the genetic diversity of phenotypic and quality traits of 48Germplasm
resources of Lycium ruthenicum Murr［J］.Journal of Henan Agricultural Sciences，2023，52（9）：78⁃90.
［4］王慧娟，王二强，符真珠，等.芍药不同品种群种质资源表型多样性分析［J］.河南农业科学，2022，51（12）：110⁃121.
WANG H J，WANG E Q，FU Z Z，et al.Phenotypic diversity analysis of different cultivars groups of herbaceous peony germplasm resources［J］.Journal of Henan Agricultural Sciences，2022，51（12）：110⁃121.
［5］于跃，孙健，张静，等.198份大麻种质资源农艺及品质性状综合评价［J］.植物遗传资源学报，2021，22（4）：1021⁃1030.
YU Y，SUN J，ZHANG J，et al.Comprehensive evaluation on agronomic and quality traits of 198 hemp germplasm

resources［J］.Journal of Plant Genetic Resources，2021，22（4）：1021⁃1030.

［6］刘思辰，曹晓宁，王海岗，等. 谷子产量性状与碾磨品质性状的相关分析［J］. 核农学报，2023，37（6）：1150⁃1159.
LIU S C，CAO X N，WANG H G，et al. Correlation between yield and milling quality traits of foxtail millet［J］.Journal of Nuclear Agricultural Sciences，2023，37（6）：1150⁃1159.

［7］莫言玲，张文静，罗亚兰，等.宽柄芥种质资源农艺性状与营养品质性状鉴定与评价［J］. 浙江农业学报，2022，34（2）：317⁃328.
MO Y L，ZHANG W J，LUO Y L，et al.Identification and evaluation of agronomic traits and nutritional quality traits
in wide handle mustard germplam resources［J］.Acta Agriculturae Zhejiangensis，2022，34（2）：317⁃328.

［8］白雪花，王延周，魏忆萍，等.298份苎麻种质资源纤维产量性状综合评价［J］.植物遗传资源学报，2022，23 （1）：106⁃122.
BAI X H，WANG Y Z，WEI Y P，et al.Comprehensive evaluation of fiber yield traits of 298 ramie germplasm resources［J］. Journal of Plant Genetic Resources，2022，23（1）：106⁃122.

［9］周远航，鲁伟丹，马小龙，等. 籽瓜种质资源表型性状鉴定及遗传多样性分析［J］. 中国农业大学学报，2023，28（8）：119⁃132.
ZHOU Y H，LU W D，MA X L，et al.Phenotypic characters identification and genetic diversity analysis of seed melon germplasm resources［J］.Journal of China Agricultural University，2023，28（8）：119⁃132.

［10］许兰杰，梁慧珍，余永亮，等.基于农艺和品质性状的宛艾种质综合评价［J］.河南农业科学，2022，51（8）：55⁃63.
XU L J，LIANG H Z，YU Y L，et al.Comprehensive Evaluation of Wan Artemisia argyi Germplasm Based on Morphological traits and Quality traits［J］.Journal of Henan Agricultural Sciences，2022，51（8）：55⁃63.

［11］黄杨，熊信果，邹小云，等.江西地方花生种质资源主要农艺性状分析与评价［J］.植物遗传资源学报，2021，22（6）：1550⁃1558.
HUANG Y，XIONG X G，ZOU X Y，et al.Analysis and evaluation of main agronomic traits of local peanut germplasms in Jiangxi Province［J］.Journal of Plant Genetic Resources，2021，22（6）：1550⁃1558.

［12］李洁，聂红民，陈翠霞，等.94个花生新品种主要农艺性状遗传多样性分析［J］.花生学报，2023，52（2）：61⁃67.
LI J，NIE H M，CHEN C X，et al. Genetic diversity of major agronomic traits in 94 new peanut cultivars［J］.Journal of Peanut Science，2023，52（2）：61⁃67.

［13］张小利，朱灵龙，李付振，等.115份花生种质资源农艺与品质性状鉴评及分析［J］.浙江农业学报，2023，35 （9）：2033⁃2044.
ZHANG X L，ZHU L L，LI F Z，et al. Evaluation and analysis of agronomic and quality traits of 115 peanut
germplasm resources［J］.Acta Agriculturae Zhejiangensis，2023，35（9）：2033⁃2044.

［14］成良强，郭建斌，吕建伟，等.30份红皮花生资源的品质性状分析［J］.花生学报，2022，51（1）：42⁃48.
CHENG L Q，GUO J B，LÜ J W，et al. Analysis on quality traits of 30 red⁃skinned peanut resources［J］.Journal of Peanut Science，2022，51（1）：42⁃48.
［15］黄怀玲，王明晓，赵传志，等.花生种质资源籽仁重量和形状的变异分析及标记筛选［J/OL］. 分子植物育种，2022：1⁃14（2022⁃07⁃07）［2024⁃05⁃24］.https：//kns.cnki.net/kcms/detail/46.1068.S.20220706.1851.012.html.
HUANG H L，WANG M X，ZHAO C Z，et al.Variation analysis and marker screening of seed weight and shape of peanut germplasms［J/OL］. Molecular Plant Breeding，2022：1⁃14（2022⁃07⁃07）［2024⁃05⁃24］.http：//kns.cnki.net/kcms/detail/46.1068.S.20220706.1851.012.html.
［16］薛云云，田跃霞，张鑫，等.72份山西花生资源主要农艺和品质性状分析［J］.花生学报，2020，49（4）：31⁃37.
XUE Y Y，TIAN Y X，ZHANG X，et al.Analysis on main agronomic and quality traits of 72 peanut resources in Shanxi of China［J］.Journal of Peanut Science，2020，49（4）：31⁃37.
［17］杨秀丽，宁东贤，武银玉，等.花生种质资源主要数量性状的遗传多样性和相关性分析［J］.中国农学通报，2015，31（9）：82⁃87.
YANG X L，NING D X，WU Y Y，et al.Genetic diversity and correlation analysis of main quantitative character of peanut germplasm resources［J］.Chinese Agricultural Science Bulletin，2015，31（9）：82⁃87.
［18］张蕙琪，薛云云，田跃霞，等.花生RIL群体芽期耐寒性与农艺性状分析［J］.花生学报，2022，51（3）：41⁃53.
ZHANG H Q，XUE Y Y，TIAN Y X，et al.Analysis of cold tolerance and agronomic characters of peanut RIL population at germination stage［J］.Journal of Peanut Science，2022，51（3）：41⁃53.
［19］孙东雷，卞能飞，陈志德，等.花生种质资源表型性状的综合评价及指标筛选［J］. 植物遗传资源学报，2018，19（5）：865⁃874.
SUN D L，BIAN N F，CHEN Z D，et al.Comprehensive evaluation and index screening of phenotypic traits in peanut germplasm resources［J］.Journal of Plant Genetic Resources，2018，19（5）：865⁃874.
［20］盖钧镒. 试验统计方法［M］.北京：中国农业出版社，2000.
GAI J Y. Experimental statistical methods［M］.Beijing：China Agricultural Publishing House，2000.
［21］姜慧芳，段乃雄.花生种质资源描述规范和数据标准［M］.北京：中国农业出版社，2006.
JIANG H F，DUAN N X.Specification and data standards for describing peanut germplasm resources［M］.Beijing：China Agricultural Publishing House，2006.
［22］贺晨帮，宗绪晓.豌豆种质资源形态标记遗传多样性分析［J］.植物遗传资源学报，2011，12（1）：42⁃48.
HE C B，ZONG X X. Genetic diversity of pea（Pisum sativum L.）germplasm resources revealed by morphological traits［J］.Journal of Plant Genetic Resources，2011，12（1）：42⁃48.

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