Effects of Different Growth‑Promoting Fungal Fertilizers on Aroma Components，Sensory Quality and Soil Properties of Flue‑cured Tobacco

doi:10.15933/j.cnki.1004-3268.2024.06.005

Abstract

Abstract: With Yunyan 87 as the test material,a field cultivation experiment was set up，and on the basis of conventional fertilization(CK),symbiotic Richoderma harzianum(Rh),internal and external symbiotic Funneliformis mosseae(Fm),endophytic Beauveria bassiana(Bb)and their combined treatments(RF,RB,FB,RFB)were applied to explore the effects of different growth‑promoting fungi on aroma components，sensory quality and soil properties of flue‑cured tobacco,to provide some reference for the application of growth‑promoting fungal fertilizer in flue‑cured tobacco production.The results showed that the inoculation and combined application of different growth‑promoting fungi had positive effects on the total amount of non‑volatile organic acids，neutral aroma components,sensory quality and soil properties of flue‑cured tobacco.Compared with CK,the soil microbial respiration rate of related inoculation treatments(Rh,Fm,Bb,RF,RB,FB,RFB)was significantly increased by 87.93%—186.21%,and other soil indexes(the content of alkaline hydrolysis nitrogen,available phosphorus,available potassium,exchangeable calcium,exchangeable magnesium and exchangeable iron)were better in multi‑inoculation treatments(RF,RB,FB,RFB).The contents of non‑volatile organic acids(succinic acid,fumaric acid,oxalic acid,citric acid,malic acid)and neutral aroma components(aromatic amino acids,browning products,plastid pigments,cembranoids)were higher in multi‑inoculation treatments(RF,FB,RFB)containing Funneliformis mosseae.In the sensory quality,the difference of the total score of each treatment was small,but the aroma quality,aroma quantity,strength and flammability of the inoculated treatments were better.Furthermore,correlation analysis showed that soil exchangeable calcium,magnesium,iron contents were closely related to aroma components and sensory quality of flue‑cured tobacco.Principal component analysis showed that FB treament was the most conducive to improving the total amount of non‑volatile organic acids,neutral aroma components and sensory quality.In summary,the combination of Funneliformis mosseae and Beauveria bassiana was the best treatment,which could improve the quality of flue‑cured tobacco by promoting soil ion exchange performance.

Key words: Flue?cured tobacco, Arbuscular mycorrhizal fungi, Beauveria bassiana, Trichoderma, Organic acids, Aroma substances, Smoking quality

摘要： 以云烟87为试材，采用田间试验，设置常规施肥（CK），在常规施肥基础上分别施用共生型哈茨木霉（Rh）、内外共生型摩西斗管囊霉（Fm）、内生型球孢白僵菌（Bb）及其组合性处理（RF、RB、FB、RFB），探索不同促生真菌对烤烟致香成分、感官质量及土壤性质的影响，为促生真菌菌肥应用于烤烟生产提供参考。结果表明，不同促生真菌单独接种及组合施用对烤烟非挥发性有机酸总量、中性致香组分、感官品质及植烟土壤性质均有积极影响。与CK相比，相关接菌处理（Rh、Fm、Bb、RF、RB、FB、RFB）土壤微生物呼吸速率显著提高87.93%~186.21%，其他土壤指标（碱解氮、速效磷、速效钾、交换性钙、交换性镁、交换性铁含量）以多元接菌处理（RF、RB、FB、RFB）整体较优。非挥发性有机酸（丁二酸、富马酸、草酸、柠檬酸、苹果酸）与中性致香组分（芳香族氨基酸类、棕色化类、质体色素类、西柏烷类）含量以含摩西斗管囊霉的多元接菌处理（RF、FB、RFB）较高。感官质量中，各处理评吸总评分差异较小，但接菌处理香气质、香气量、劲头、燃烧性等方面表现更佳。相关性分析表明，土壤交换性钙、交换性镁、交换性铁含量与烤烟致香成分、感官质量密切相关；主成分分析排序表明，FB处理最有利于提高非挥发性有机酸总量、中性致香组分及评吸感官品质。综上，摩西斗管囊霉与球孢白僵菌组合接种处理效果较佳，其可通过增强土壤离子交换性能提高烤烟品质，是最优处理。

关键词: 烤烟, 丛枝菌根真菌, 球孢白僵菌, 木霉菌, 有机酸, 致香物质, 评吸质量

CLC Number:

S572

YE Zhihui, SHAO Xinguang, ZHAO Xiaozhong. Effects of Different Growth‑Promoting Fungal Fertilizers on Aroma Components，Sensory Quality and Soil Properties of Flue‑cured Tobacco[J]. Journal of Henan Agricultural Sciences, 2024, 53(6): 37-48.

叶志晖, 邵新光, 赵晓忠. 不同促生真菌菌肥配施对烤烟致香成分、感官质量及土壤性质的影响[J]. 河南农业科学, 2024, 53(6): 37-48.

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