Isolation and Identification of Functional Bacteria in Different Fermentation Stages of Pleurotus ostreatus

doi:10.15933/j.cnki.1004-3268.2022.02.013

Abstract

Abstract: In order to screen out dominant strains with promoting effect on the growth of Pleurotus ostreatus and inhibiting effect on Trichoderma from the fermentation material of Pleurotus ostreatus，a total of 94 strains of bacteria were isolated from the fermentation material of Pleurotus ostreatus in different periods.The isolated strains were screened for Pleurotus ostreatus promotion and Trichoderma inhibition，and 16S rRNA gene sequence analysis of the effective strains was carried out. The results showed that 24 strains had a good growth promotion effect on Heiping 17，and the highest growth promotion rate of strain PC18 belonging to Pseudoxanthomonas was 38.97%.11 strains had a strong inhibitory effect on Trichoderma，and the highest inhibitory rate of PA6 was 30.43%.11 strains had a double effect.Among them，strains PA6，PA13，PA15 and PA17 could inhibit the growth of Trichoderma，promote the growth of Pleurotus ostreatus Heiping 17，and also have the ability to produce IAA. They were all identified as Brevibacillus genus.

Key words: Pleurotus ostreatus fermentation material, Growth promotion, Identification, Inhibition, Trichoderma, Dominant bacteria

摘要： 为从平菇发酵料中分离筛选出对平菇的生长有促进作用及对木霉有抑制作用的优势菌株，从不同时期的平菇发酵料中分离出94株细菌，对分离的菌株进行平菇促进以及木霉抑制作用筛选，并对有效果的菌株进行16S rRNA基因序列分析。结果表明，24株筛选菌对黑平17具有良好的促生作用，菌株PC18促生率最高，为38.97%，属于Pseudoxanthomonas（假黄单胞菌属）。11株筛选菌对木霉有较强的抑制作用，PA6抑制率最高，为30.43%。11株筛选菌具有双重效果，其中，菌株PA6、PA13、PA15、PA17既
能抑制木霉菌生长又能促进平菇黑平17生长，同时具有产生长素IAA的能力，均被鉴定为Brevibacillus（短芽孢杆菌属）。

关键词: 平菇发酵料, 促生作用, 鉴定, 抑制作用, 木霉, 优势菌

CLC Number:

S646.1⁺4

ZHANG Junjie, LI Shuo, CUI Xiao, WANG Nan, SONG Kaibo, LIU Qin, KONG Weili. Isolation and Identification of Functional Bacteria in Different Fermentation Stages of Pleurotus ostreatus[J]. Journal of Henan Agricultural Sciences, 2022, 51(2): 113-119.

张俊杰, 李硕, 崔筱, 王楠, 宋凯博, 刘芹, 孔维丽. 平菇发酵料不同发酵阶段功能菌的分离与鉴定[J]. 河南农业科学, 2022, 51(2): 113-119.

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