Abstract: In order to explore the regulation methods for the production of aroma substances in flue-cured tobacco leaves,Yunyan 87,the main planting variety in Guizhou tobacco area,was selected to analyze the content change of the related substances of flue-cured tobacco DXP pathway(pyruvate,DXP,IPP,cembra triene diol) and some organic acids and higher fatty acids under different application rates of magnesium and manganese.The results showed that the contents of flue-cured tobacco cembra triene diol and some higher fatty acids(oleic acid,linoleic acid) showed a trend of first increasing and then decreasing with the growth process,the content of malic acid showed a gradually increasing trend,and the content of citric acid showed a tend of first decreasing and then increasing;Regarding the related products of the DXP pathway,under the condition of magnesium application, the content of β-cembra triene diol had no significant correlation with the content of DXP and IPP,but there was a significant or extremely significant positive correlation between the other indicators;Under the condition of manganese application,there was no significant correlation between the content of DXP and the contents of pyruvate,IPP,and β-cembra triene diol, but there was a significant or extremely significant positive correlation among the other indicators.Compared with CK,applying magnesium and manganese could increase the content of DXP pathway related substances and some organic acids and higher fatty acids in tobacco leaves to varying degrees,and increase with the increase of application rate within the application range of 0—20 g/plant, magnesium sulfate was more effective than manganese sulfate;It was the best effect when the application rate of magnesium sulfate was 20 g/plant,the content of(α,β)-cembra triene diol in mature flue-cured tobacco increased by 11.99% and 36.72%,respectively,and the content of malic acid and citric acid contents increased by 21.77% and 19.71%,respectively;The content of oleic acid and linoleic acid increased by 27.46% and 43.38%, respectively.In summary, the application of magnesium and manganese are beneficial to the accumulation of related substances in the DXP pathway and organic acid of flue-cured tobacco, which is expected to promote the synthesis of aroma substances in flue-cured tobacco and improve the quality of flue-cured tobacco.

Key words: Flue-cured tobacco, Magnesium, Manganese, DXP, Organic acids, Higher fatty acid

摘要： 为探究烤烟烟叶香气物质产生的调控方法，选取贵州烟区主栽品种云烟87，分析镁、锰不同施用量条件下烤烟1-脱氧-D-木酮糖-5-磷酸(DXP)途径相关物质（丙酮酸、DXP、IPP、西柏三烯二醇）及部分有机酸、高级脂肪酸含量的变化。结果表明：烤烟西柏三烯二醇及部分高级脂肪酸（油酸、亚油酸）含量随生育进程均表现出先增加后降低的趋势，苹果酸含量表现出逐渐升高的趋势，柠檬酸含量表现为先降低后升高的趋势；就DXP途径相关产物而言，施镁条件下，β-西柏三烯二醇含量与DXP、IPP含量无显著相关性，但其余各项指标间均呈显著或极显著正相关关系；施锰条件下，DXP含量与丙酮酸、IPP、β-西柏三烯二醇含量无显著相关性，但其余各项指标间呈显著或极显著正相关关系；与CK相比，施用镁、锰均在不同程度上提高烟叶DXP途径相关物质及部分有机酸、高级脂肪酸的含量，且在施用量0～20 g/株内随施用量增加而增加，施硫酸镁比施硫酸锰效果更好；硫酸镁施用量20 g/株时，成熟期烤烟(α、β)-西柏三烯二醇含量较CK(不施镁、锰)分别提高了11.99%、36.72%，苹果酸、柠檬酸含量分别提高21.77%、19.71%，油酸、亚油酸含量分别提高27.46%、43.38%，整体效果最好。综上所述，施用镁、锰有利于烤烟DXP途径相关物质和有机酸的累积，有望促进烤烟香气物质的合成，改善烤烟品质。

关键词: 烤烟, 镁, 锰, 1-脱氧-D-木酮糖-5-磷酸, 有机酸, 高级脂肪酸