Effects of Carbon and Phosphorus Addition on the Characteristics and Construction Process of Microbial Communities in the Yellow Soil of Central Guizhou

doi:10.15933/j.cnki.1004-3268.2025.07.009

Abstract

Abstract: To investigate the effects of external carbon and phosphorus on the characteristics and construction process of soil microbial communities，glucose was used as the exogenous carbon source material，and potassium dihydrogen phosphate was used as the phosphorus source. Four treatments were set up，including control（CK），carbon addition（C），phosphorus addition（P），and carbon phosphorus addition（CP）.Using yellow soil from central Guizhou as the experimental soil，a pot experiment with oilseed rape was conducted to investigate the effects of carbon and phosphorus addition on soil physicochemical properties，microbial community structure，and community assembly.The results showed that the total nitrogen content of the C，P，and CP treatments increased significantly by 7.82%，6.17%，and 6.17%，respectively，compared to the CK treatment，with no significant difference among the three treatments.The content of soil alkali hydrolyzable nitrogen in C，P and CP treatments increased，but there was no significant difference compared with the control.The soil nitrate nitrogen content in C and P treatments was significantly higher than that in CK and CP treatments.The soil pH value and ammonium nitrogen content in CP treatment were significantly higher than those in other treatments.The oilseed rape yields of the treatments were in the order of CP>C>P>CK.The relative abundance of Proteobacteria and Actinobacteria decreased，while the relative abundance of Chloroflexi and Gemmatimonadetes increased.The addition of carbon and phosphorus promoted the growth of Ascomycota.The addition of carbon and phosphorus had little effect on the α diversity of bacterial and fungal communities.The Mantel test showed that indicators such as soil ammonium nitrogen，organic matter，and available potassium content had a significant impact on bacterial community beta diversity at the P<0.001 level；The indicators of soil ammonium nitrogen and total phosphorus content had a significant impact on the β diversity of fungal communities at the P<0.01 level.The order of smoothness of bacterial network connectivity was P>C>CP>CK，while the order of smoothness of fungal network connectivity was C>P>CK>CP.The construction process of bacterial and fungal communities in each treatment was dominated by stochastic processes.The content of ammonium nitrogen was significantly correlated with bacterial and fungal βNTI values at the P<0.001 level，while pH value and soil organic matter were significantly correlated with bacterial βNTI values（P<0.05 or more significan）and fungal βNTI values at the P<0. 001 level. In summary，the addition of carbon and phosphorus has an impact on soil physical and chemical properties（such as ammonium nitrogen），thereby altering microbial community β diversity and community construction processes.

Key words: Yellow soil, Microbial community, Community assembly, Carbon and phosphorus addition, Response

摘要： 为探究外加碳磷对土壤微生物群落特征及构建过程的影响，以葡萄糖作为外加碳源物质，同时以磷酸二氢钾作为磷源，设置空白（CK）、碳添加（C）、磷添加（P）、碳磷添加（CP）等4个处理，以黔中黄壤为试验土壤，通过盆栽油菜试验研究碳磷添加对土壤理化性质、微生物群落结构及群落构建的影响。结果表明，C、P和CP处理土壤全氮含量分别比CK处理显著提高7.82%、6.17%、6.17%，3个处理之间差异不显著；C、P和CP处理土壤碱解氮含量提高，但与对照差异均不显著；C和P处理土壤硝态氮含量均显著高于CK和CP处理；CP处理土壤pH值和铵态氮含量均显著高于其他处理。不同处理油菜鲜质量大小依次为CP>C>P>CK。外加碳磷细菌变形菌门（Proteobacteria）、放线菌门（Actinobacteria）的相对丰度降低，绿弯菌门（Chloroflexi）、芽单胞菌门（Gemmatimonadetes）相对丰度提高；外加碳磷促进了真菌子囊菌门（Ascomycota）的生长。外加碳磷对细菌和真菌群落α多样性影响较小，曼特尔检验（Mantel test）表明土壤铵态氮、有机质、速效钾含量等指标在P<0.001显著水平上对细菌群落β多样性产生影响；土壤铵态氮、全磷含量等指标在P<0.01显著水平上对真菌群落β多样性产生影响。细菌网络连通平稳度大小依次为P>C>CP>CK，真菌网络连通平稳度大小依次为C>P>CK>CP。各处理细菌和真菌群落构建过程均以随机性过程占主导，铵态氮含量与细菌和真菌βNTI（β-nearest taxon index）值均在P<0.001显著水平上相关，pH值、有机质含量与细菌βNTI值均在P<0.05及以上显著水平上相关，与真菌βNTI值在P<0.001显著水平上相关。综上所述，外加碳磷对土壤理化性质（铵态氮等）产生影响，进而改变微生物群落β多样性和群落构建过程。

关键词: 黄壤, 微生物群落, 群落构建, 碳磷添加, 响应

CLC Number:

S154.3

QIN Shihua, CONG Chunlei, YAN Ru, LIU Chao, SONG Xiaohui, QIAO Zhiwei. Effects of Carbon and Phosphorus Addition on the Characteristics and Construction Process of Microbial Communities in the Yellow Soil of Central Guizhou[J]. Journal of Henan Agricultural Sciences, 2025, 54(7): 84-97.

秦仕华, 从春蕾, 闫茹, 刘超, 宋晓慧, 乔志伟. 外加碳磷对黔中黄壤微生物群落特征及构建过程的影响[J]. 河南农业科学, 2025, 54(7): 84-97.

Figures/Tables 10

References

［1］魏全全，张萌，芶久兰，等.控释尿素和有机物料添加提高黄壤冬油菜产量及养分吸收利用［J］.中国土壤与肥料，2024（1）：113‑121.
WEI Q Q，ZHANG M，GOU J L，et al. Controlled‑release urea and organic materials can increase the yield and nutrient absorption and utilization of winter rapeseed in yellow soil［J］.Soil and Fertilizer Sciences in China，2024（1）：113‑121.

［2］刘方，龙永根，刘元生，等. 旱期水肥耦合对贵州山区辣椒生长及产量的影响［J］.中国土壤与肥料，2014（6）：59‑62.

LIU F，LONG Y G，LIU Y S，et al. Effect of water and fertilizer coupling on growth and yield of chili at dry period［J］. Soil and Fertilizer Sciences in China，2014（6）：59‑62.

［3］NIE Z J，LI J F，LIU H Y，et al. Adsorption kinetic characteristics of molybdenum in yellow‑brown soil in response to pH and phosphate［J］. Open Chemistry，2020，18（1）：663‑668.

［4］张萌，刘彦伶，魏全全，等.酒糟生物炭短期施用对贵州黄壤氮素有效性及细菌群落结构多样性的影响［J］. 环境科学，2020，41（10）：4690‑4700.

ZHANG M，LIU Y L，WEI Q Q，et al.Effects of short‑term application of Moutai‑flavor vinasse biochar on nitrogen availability and bacterial community structure diversity in yellow soil of Guizhou Province［J］.Environmental Science，2020，41（10）：4690‑4700.

［5］DELGADO‑BAQUERIZO M， BARDGETT R D，VITOUSEK P M，et al.Changes in belowground biodiversity during ecosystem development［J］.Proceedings of the National Academy of Sciences of the United States of America，2019，116（14）：6891‑6896.

［6］陈鲜妮，米倩，徐晓峰. 局域尺度麦田土壤微生物群落构建过程及其驱动因素［J］.土壤，2023，55（4）：812‑820.

CHEN X N，MI Q，XU X F. Soil microbial community construction processes and its driving factors in wheat field at local‑scale［J］. Soils，2023，55（4）：812‑820.

［7］郭晗玥，王东升，阮杨，等. 西瓜连作根际土壤微生物群落演替特征［J］.中国农业科学，2023，56（21）：4245‑4258.

GUO H Y，WANG D S，RUAN Y，et al. Characteristics and succession of rhizosphere soil microbial communities in continuous cropping watermelon［J］.Scientia Agricultura Sinica，2023，56（21）：4245‑4258.

［8］ETESAMI H，JEONG B R，GLICK B R. Contribution of arbuscular mycorrhizal fungi，phosphate‑solubilizing bacteria，and silicon to P uptake by plant［J］.Frontiers in Plant Science，2021，12：699618.

［9］XIONG C，ZHU Y G，WANG J T，et al. Host selection shapes crop microbiome assembly and network complexity［J］.New Phytologist，2021，229（2）：1091‑1104.

［10］SHELDRAKE M，ROSENSTOCK N P，REVILLINI D，et al. A phosphorus threshold for mycoheterotrophic plants in tropical forests［J］. Proceedings Biological Sciences，2017，284（1848）：20162093.

［11］李红宇，张巩亮，范名宇，等.生物炭连续还田对苏打盐碱水稻土养分及真菌群落结构的影响［J］.水土保持学报，2020，34（6）：345‑351.

LI H Y，ZHANG G L，FAN M Y，et al.Effects of continuous biochar returning on nutrients of soda saline‑alkali paddy soil and fungal community structure［J］.Journal of Soil and Water Conservation，2020，34 （6）：345‑351.

［12］杨璐，曾闹华，白金顺，等. 紫云英季土壤固氮微生物对外源碳氮投入的响应［J］.中国农业科学，2020，53（1）：105‑116.

YANG L，ZENG N H，BAI J S，et al. Responses of soil diazotroph community to rice straw，glucose and nitrogen addition during Chinese milk vetch growth［J］.Scientia Agricultura Sinica，2020，53（1）：105‑116.

［13］常芳娟，张贵云，张丽萍，等. 生物炭对西瓜连作土壤真菌群落结构和功能类群的影响［J］.环境科学，2024，45（6）：3553‑3561.

CHANG F J，ZHANG G Y，ZHANG L P，et al. Effects of biochar application on the structure and function of fungal community in continuous cropping watermelon soil［J］.Environmental Science，2024，45（6）：3553‑3561.

［14］BASTIDA F，TORRES I F，MORENO J L，et al.The active microbial diversity drives ecosystem multifunctionality and is physiologically related to carbon availability in Mediterranean semi‑arid soils［J］.Molecular Ecology，2016，25（18）：4660‑4673.

［15］GUO R H，JIN H M，CHANG Z Z. et al. Effects of returning patterns of straw to field on soil organic carbon and soil humus composition in rice‑wheat double cropping systems［J］.Journal of Agro‑Environment Science，2017，36（4）：727‑733.

［16］HOU E Q，LUO Y Q，KUANG Y W，et al.Global meta‑analysis shows pervasive phosphorus limitation of aboveground plant production in natural terrestrial ecosystems［J］.Nature Communications，2020，11：637.

［17］卢孟雅，王智权，张昆，等. 氮磷对秸秆输入下稻田土壤微生物群落及其残体的影响［J/OL］.土壤学报，2024：1‑14（2024‑09‑19）［2025‑02‑10］. http：//kns.cnki. net/KCMS/detail/detail. aspx？filename=TRXB20240914005&dbname=CJFD&dbcode=CJFQ.

LU M Y，WANG Z Q，ZHANG K，et al. Effects of nitrogen and phosphorus on soil microbial community and its residues in paddy field under straw input［J/OL］. China Industrial Economics， 2024： 1‑14 （2024‑09‑19）［2025‑02‑10］. http：//kns. cnki. net/KCMS/detail/detail. aspx？filename=TRXB20240914005&dbname=CJFD&dbcode=CJFQ.

［18］FIERER N，LADAU J，CLEMENTE J C，et al.Reconstructing the microbial diversity and function of pre‑agricultural tallgrass prairie soils in the United States［J］. Science，2013，342（6158）：621‑624.

［19］刘珊珊，王全成，史加勉，等.亚热带森林菌根植物根系真菌群落结构对氮磷添加的响应［J］.应用生态学报，2023，34（6）：1547‑1554.

LIU S S，WANG Q C，SHI J M，et al.Responses of root‑associated fungal community structure of mycorrhizal plants to nitrogen and/or phosphorus addition in a subtropical forest［J］.Chinese Journal of Applied Ecology，2023，34（6）：1547‑1554.

［20］邓慧玉，刘子恺，马星竹，等.长期施肥处理对东北黑土真菌多样性和群落结构的影响［J］.福建师范大学学报（自然科学版），2024，40（1）：69‑75.

DENG H Y，LIU Z K，MA X Z，et al. Effect of long‑term fertilization on black soil fungal community diversity and structure in NorthEast China［J］. Journal of Fujian Normal University（Natural Science Edition），2024，40（1）：69‑75.

［21］WU W C，WANG F，XIA A Q，et al. Meta‑analysis of the impacts of phosphorus addition on soil microbes［J］.Agriculture，Ecosystems & Environment，2022，340：108180.

［22］MA X M，ZHOU Z，CHEN J，et al. Long‑term nitrogen and phosphorus fertilization reveals that phosphorus limitation shapes the microbial community composition and functions in tropical montane forest soil［J］.Science of the Total Environment，2023，854：158709.

［23］JIAO S，PENG Z H，QI J J，et al. Linking bacterial‑fungal relationships to microbial diversity and soil nutrient cycling［J］. mSystems，2021，6（2）：e01052‑20.

［24］刘玉槐，魏晓梦，魏亮，等. 水稻根际和非根际土磷酸酶活性对碳、磷添加的响应［J］.中国农业科学，2018，51（9）：1653‑1663.
LIU Y H，WEI X M，WEI L，et al. Responses of extracellular enzymes to carbon and phosphorus additions in rice rhizosphere and bulk soil［J］.Scientia Agricultura Sinica，2018，51（9）：1653‑1663.

［25］薛美瑛，李春越，党廷辉，等. 碳磷添加对黄土区农田土壤呼吸及温度敏感性的影响［J］.地球环境学报，2019，10（1）：69‑78.
XUE M Y，LI C Y，DANG T H，et al. Effects of carbon and phosphorus additions on soil respiration and temperature sensitivity of farmland in the loess region［J］.Journal of Earth Environment，2019，10（1）：69‑78.

［26］徐鹏，邬磊，胡金丽，等. 添加葡萄糖、乙酸、草酸对红壤旱地土壤氮素矿化及反硝化的影响［J］.环境科学学报，2017，37（12）：4740‑4746.
XU P，WU L，HU J L，et al. Effects of glucose，acetic acid and oxalic acid additions on nitrogen mineralization and denitrification in red upland soil ［J］. Acta Scientiae Circumstantiae，2017，37（12）：4740‑4746.

［27］鲍士旦. 土壤农化分析［M］. 3版. 北京：中国农业出版社，2000.
BAO S D. Soil agrochemical analysis［M］. 3rd ed.Beijing：China Agricultural Press，2000.

［28］贾俊仙，李忠佩，车玉萍. 添加葡萄糖对不同肥力红壤性水稻土氮素转化的影响［J］.中国农业科学，2010，43（8）：1617‑1624.
JIA J X，LI Z P，CHE Y P. Effects of glucose addition on N transformations in paddy soils with a gradient of organic C content in subtropical China［J］.Scientia Agricultura Sinica，2010，43（8）：1617‑1624.

［29］SIEGEL C S，STEVENSON F O，ZIMMER E A.Evaluation and comparison of FTA card and CTAB DNA extraction methods for non‑agricultural taxa［J］.Applications in Plant Sciences，2017，5（2）：1600109.

［30］CALLAHAN B J，MCMURDIE P J，ROSEN M J，et al.DADA2： High‑resolution sample inference from illumina amplicon data［J］. Nature Methods，2016，13 （7）：581‑583.

［31］BOKULICH N A，KAEHLER B D，RIDEOUT J R，et al. Optimizing taxonomic classification of marker‑gene amplicon sequences with QIIME 2’s q2‑feature‑classifier plugin［J］.Microbiome，2018，6（1）：90.

［32］PENG G S，WU J. Optimal network topology for structural robustness based on natural connectivity［J］.Physica A：Statistical Mechanics and Its Applications，2016，443：212‑220.

［33］黄祎，王鹏，高鹏飞，等.南昌城市湖泊浮游微生物群落构建机制及影响因素［J］.环境科学学报，2023，43（8）：407‑417.
HUANG Y，WANG P，GAO P F，et al. Mechanisms of planktonic microbial community assembly and influencing factors in Nanchang urban lakes［J］.Acta Scientiae Circumstantiae，2023，43（8）：407‑417.

［34］REED S C，CLEVELAND C C，TOWNSEND A R.Functional ecology of free‑living nitrogen fixation：A contemporary perspective［J］.Annual Review of Ecology， Evolution， and Systematics， 2011， 42：489‑512.

［35］BOOTH M S，STARK J M，RASTETTER E. Controls on nitrogen cycling in terrestrial ecosystems：A synthetic analysis of literature data［J］.Ecological Monographs，2005，75（2）：139‑157.

［36］ZHENG M H，XU M C，LI D J，et al. Negative responses of terrestrial nitrogen fixation to nitrogen addition weaken across increased soil organic carbon levels［J］.Science of the Total Environment，2023，877：162965.

［37］SULIEMAN S，HA C V，SCHULZE J，et al. Growth and nodulation of symbiotic Medicago truncatula at different levels of phosphorus availability［J］.Journal of Experimental Botany，2013，64（10）：2701‑2712.

［38］DIVITO G A，SADRAS V O. How do phosphorus，potassium and sulphur affect plant growth and biological nitrogen fixation in crop and pasture legumes？A meta‑analysis［J］. Field Crops Research，2014，156：161‑171.

［39］AUGUSTO L，DELERUE F，GALLET‑BUDYNEK A，et al.Global assessment of limitation to symbiotic nitrogen fixation by phosphorus availability in terrestrial ecosystems using a meta‑analysis approach［J］. Global Biogeochemical Cycles，2013，27（3）：804‑815.

［40］MAISTRY P M，CRAMER M D，CHIMPHANGO S B.N and P colimitation of N2‑fixing and N‑supplied fynbos legumes from the Cape Floristic Region［J］.Plant and Soil，2013，373（1）：217‑228.

［41］BURGER M，JACKSON L E. Microbial immobilization of ammonium and nitrate in relation to ammonification and nitrification rates in organic and conventional cropping systems［J］. Soil Biology and Biochemistry，2003，35（1）：29‑36.

［42］WANG J，SUN N，XU M G，et al.The influence of long‑term animal manure and crop residue application on abiotic and biotic N immobilization in an acidified agricultural soil［J］. Geoderma，2019，337：710‑717.

［43］DUAN Y H，XU M G，WANG B R，et al. Long‑term evaluation of manure application on maize yield and nitrogen use efficiency in China［J］. Soil Science Society of America Journal，2011，75（4）：1562‑1573.

［44］苏渝钦，刘何铭，郑泽梅，等. 氮磷添加对中亚热带常绿阔叶林土壤有效氮和pH值的影响［J］.生态学杂志，2016，35（9）：2279‑2285.
SU Y Q，LIU H M，ZHENG Z M，et al. Effects of N and P addition on soil available nitrogen and pH in a subtropical forest［J］. Chinese Journal of Ecology，2016，35（9）：2279‑2285.

［45］WANG Q，WANG C，YU W W，et al. Effects of nitrogen and phosphorus inputs on soil bacterial abundance，diversity，and community composition in Chinese fir plantations［J］. Frontiers in Microbiology，2018，9：1543.

［46］许秋月，何敏，夏允，等. 磷添加对森林土壤有机碳影响的研究进展［J］.亚热带资源与环境学报，2024，19（1）：9‑15.
XU Q Y，HE M，XIA Y，et al. Research progress on effects of phosphorus addition on forest soil organic carbon［J］. Journal of Subtropical Resources and Environment，2024，19（1）：9‑15.

［47］夏允. 磷添加对杉木人工林土壤微生物群落结构和功能的影响［D］. 福州：福建师范大学，2022.

XIA Y. Effects of phosphorus addition on soil microbial community structure and function of Chinese fir plantation［D］. Fuzhou：Fujian Normal University，2022.

［48］LIANG Y T，ZHAO H H，DENG Y，et al. Long‑term oil contamination alters the molecular ecological networks of soil microbial functional genes［J］.Frontiers in Microbiology，2016，7：60.

［49］LEI L S，GU J，WANG X J，et al. Microbial succession and molecular ecological networks response to the addition of superphosphate and phosphogypsum during swine manure composting［J］. Journal of Environmental Management，2021，279：111560.

［50］COYLE J R，HALLIDAY F W，LOPEZ B E，et al.Using trait and phylogenetic diversity to evaluate the generality of the stress‑dominance hypothesis in eastern North American tree communities［J］.Ecography，2014，37（9）：814‑826.

［51］ZHAN P F，LIU Y S，WANG H C，et al. Plant litter decomposition in wetlands is closely associated with phyllospheric fungi as revealed by microbial community dynamics and co‑occurrence network［J］.Science of the Total Environment，2021，753：142194.

［52］景秀清，赵鹏宇，白雪，等. 谷田土壤中细菌群落对铬胁迫的响应特征［J］.农业环境科学学报，2023，42（6）：1294‑1304.
JING X Q，ZHAO P Y，BAI X，et al.Response characteristics of bacterial communities to chromium stress in the soil of millet fields［J］.Journal of Agro‑Environment Science，2023，42（6）：1294‑1304.

［53］吴宪，胡菏，王蕊，等. 化肥减量和有机替代对潮土微生物群落分子生态网络的影响［J］.土壤学报，2022，59（2）：545‑556.
WU X，HU H，WANG R，et al. Effects of reduction of chemical fertilizer and substitution coupled with organic manure on the molecular ecological network of microbial communities in fluvo‑aquic soil［J］.Acta Pedologica Sinica，2022，59（2）：545‑556.

［54］CHEN Q L，HU H W，YAN Z Z，et al. Deterministic selection dominates microbial community assembly in termite mounds［J］. Soil Biology and Biochemistry，2021，152：108073.

［55］WU W X，LU H P，SASTRI A，et al. Contrasting the relative importance of species sorting and dispersal limitation in shaping marine bacterial versus protist communities［J］.The ISME Journal，2018，12（2）：485‑494.

[1]	QIAO Zhiwei, ZHANG Yongjie, XIE Meifang. Phosphorus Solubilizing Characteristics of Phosphorus Solubilizing Bacteria in Yellow Soil of Central Guizhou and Their Effects on Soil Microbial Carbon Cycling Genes [J]. Journal of Henan Agricultural Sciences, 2023, 52(8): 78-86.
[2]	WEI Quanquan, GU Xiaofeng, GOU Jiulan, ZHANG Meng, RAO Yong, XIAO Huagui. Effect of Nitrogen Fertilizer Combined with Organic Materials on Microbial Community in Winter Rapeseed⁃Maize Rotation in Yellow Soil [J]. Journal of Henan Agricultural Sciences, 2023, 52(6): 41-50.
[3]	REN Yuansen, ZHANG Haibo, CHENG Hongyan, WANG Yumeng, LUO Yuan, LIU Na. Effect of pH Value on Acid Production Characteristics and Microbial Community in Co‐Fermentation of Pig Manure and Spent Mushroom Substrate [J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 110-120.
[4]	WEI Quanquan, ZHANG Meng, LIU Lingling, GU Xiaofeng, QIN Song, GOU Jiulan. Effects of Leguminous Green Manure Return and Nitrogen Fertilizer Reduction on Maize Growth in Yellow Soil [J]. Journal of Henan Agricultural Sciences, 2023, 52(4): 66-73.
[5]	HOU Qiang, ZHOU Xin, ZHANG Jianxiong, MA Ruixin, WU Jiang’ai, CUI Ya’nan, CHEN Yu, ZHENG Baiqin, ZHANG Lifang. Comparative Analysis of the Microbial Community Structure in Litopenaeus vannamei Intestine and Surrounding Environment from Different Culture Patterns [J]. Journal of Henan Agricultural Sciences, 2023, 52(11): 133-145.
[6]	. Analysis of Flavor Characteristics and Microbial Community of Cigar Tobacco Leaves from Different Regions [J]. Journal of Henan Agricultural Sciences, 2022, 51(8): 45-54.
[7]	WU Fengxia, SUN Yue, XIAO Qiang, ZHANG Shubin, LI Yufei, LIU Jianbin. Degradation Ability and Influencing Factors of Cellulose‑degradation Microbial Community PN‑8 on Wheat Straw [J]. Journal of Henan Agricultural Sciences, 2022, 51(4): 77-86.
[8]	SONG Yu . Association Analysis of Rhizosphere Soil Microbial Community and Rice Yield in Rice‑Crab Farming System [J]. Journal of Henan Agricultural Sciences, 2022, 51(10): 86-95.
[9]	WU Yaoling, XI Xiaoli, ZENG Xianglian, LÜ Xibin, WANG Heyu, WANG Li. Isolation and Compost Application of High‐Activity Cellulose‐degrading Strains from Distiller’s Grains [J]. Journal of Henan Agricultural Sciences, 2022, 51(1): 79-88.
[10]	ZHANG Qin, YAO Danjun, ZHANG Jumei, NIU Yaqiong, WANG Wenhua, ZHANG Aihua, KUANG Shengjian, LIAO Heng, ZHU Qing. Characteristics of Growth and Utilization and Seed Yield of Green Manure Germplasm Resources Introduced from Abroad in Low⁃Phosphorus Yellow Soil of Guizhou Province [J]. Journal of Henan Agricultural Sciences, 2021, 50(9): 71-78.
[11]	LIU Hancheng, KONG Zhaoxia, LONG Xueping, LI Baocheng, MA Xiaomei, ZHA Xizongji, LIU Jing. Study on Physical and Chemical Properties and Microbial Community Composition in Root Soil of Rheum tanguticum [J]. Journal of Henan Agricultural Sciences, 2021, 50(4): 56-65.
[12]	YU Jingjing, CAI Debao, CHEN Xiuwen, ZHANG Jun, CHEN Jibao. Effect of Biochar and Humic Acid Addition on the Soil Aggregate of Danjiangkou Reservoir [J]. Journal of Henan Agricultural Sciences, 2021, 50(11): 87-96.
[13]	ZHAO Hui, WANG Xiying, XU Shiqiang, TAN Zhiyong. Soil Microbial Community Structure and Functional Diversity in Vegetable Greenhouse for Different Planting Years in Wuling Area of Guizhou Province [J]. Journal of Henan Agricultural Sciences, 2021, 50(1): 81-91.
[14]	LI Guirong, CHEN Fukai, JIA Shengyong, WANG Zongshuo, GUO Zenan. Influens of Eggplant Straw-based Biochar Comined with Ryegrass on Remediation of Cd and Pyrene Co-contaminations in Soil [J]. Journal of Henan Agricultural Sciences, 2020, 49(9): 51-61.
[15]	FAN Fangfang, BAI Wenbin, WANG Lei, WANG Jinsong, NIE Meng’en, LIU Peng, LI Guang, FAN Xinqi. Effects of Different Control Measures on the Growth and Rhizosphere Soil Microbial Community Structure of Continuous Cropping Sorghum [J]. Journal of Henan Agricultural Sciences, 2020, 49(10): 70-77.