Effect of Biochar and Humic Acid Addition on the Soil Aggregate of Danjiangkou Reservoir

doi:10.15933/j.cnki.1004-3268.2021.11.011

Abstract

Abstract: The influences of biochar and humic acid on soil aggregate stability and microbial community structure of Danjiangkou Reservoir were evaluated in this study.Soil samples were incubated outdoor for 12 months by adding 0，150，300 kg/ha of humic acid（H0，H1，H2）and 0，750，1 500 kg/ha of biochar（B0，B1，B2）. Then，sieving methods were used to detect the distribution of soli aggregates composition，the mean weight diameter（MWD）and percentage of aggregate disruption（PAD）.The numbers of soil fungi，bacteria and actinomycetes were measured by microbial dilution plate counting cultivation method.The results showed that addition of biochar and humic acid significantly impacted the soil aggregate composition. With the increase of biochar and humic acid addition，the amount of >0. 5 mm water stability aggregate increased and <0. 5 mm water stability aggregate decreased gradually. Compared with the control treatment without addition of biochar and humic acid，under only addition of humic acid or biochar，or the combined application of both，the average amount of >0.5 mm water stability aggregate increased by 25.4%，115.5%，135.9%，the average of MWD increased by 19.5%，93.0%，110.2%，the average of PAD reduced by 14.1%，47.3%，55.2%，the average amount of bacteria increased by 4.2%，129.8%，139.8%，the average amount of actinomycetes increased by 12.7%，77.8%，99.3%，the average amount of fungi increased by 20.7%，77.7%，80.4%，respectively. Among them，the average amount of >0.5 mm water stability aggregate，bacteria，actimomycetes and fungi was the highest in B2H2 treatment，which increased by 183.3%，199.8%，121.2%，88.5%，respectively compared with the control.In general，adding humic acid or biochar can enhance the formation of macroaggregates（>0.25 mm），improve the stability of soil structure and increase the abundance of soil culturable microorganisms of Danjangkou Reservoir.The combined application of biochar and humic acid was more helpful to promote the formation of soil macroaggregates and the growth of microorganisms than the single addition.

Key words: Soil aggregate, Biochar, Humic acid, Microbial community, Danjiangkou Reservoir

摘要： 为了解生物炭（B）和腐植酸（H）施用对丹江口库区土壤团聚体稳定性和微生物群落的影响，采用室外培养试验，向土中同时分别添加0、150、300 kg/hm²的腐植酸（H0、H1、H2）和0、750、1 500 kg/hm²的生物炭（B0、B1、B2），12个月后用筛分法对土壤团聚体结构分布、平均质量直径（MWD）、团聚体破坏率（PAD）进行分析，并采用稀释平板计数法测定土壤中细菌、真菌、放线菌的数量。结果表明，添加生物炭和腐植酸均可显著改变土壤团聚体组成，且随着生物炭和腐植酸添加量的增加，>0.5 mm水稳定性团聚体含量逐渐增高，<0.5 mm的水稳定性团聚体含量逐渐降低。和未施用腐植酸和生物炭的对照相比，单独施用腐植酸或生物炭或二者耦合施用处理下，土壤>0.5 mm 水稳定性团聚体平均含量分别增加25.4%、115.5%、135.9%，MWD均值分别增加19.5%、93.0%、110.2%，PAD均值分别降低14.1%、47.3%、55.2%，细菌平均分别增加4.2%、129.8%、139.8%，放线菌平均分别增加12.7%、77.8%、99.3%，真菌平均分别增加20.7%、77.7%、80.4%。其中，B2H2耦合处理对>0.5 mm水稳定性团聚体的形成及细菌、放线菌和真菌的生长具有最大促进作用，分别比对照提高183.3%、199.8%、121.2%、88.5%。综上，土壤中添加腐植酸或生物炭有助于促进大团聚体（>0.25 mm）的形成，可提高丹江口库区土壤结构的稳定性，增加土壤可培养微生物的丰度；生物炭和腐植酸耦合施用比单独施用更有助于促进土壤大团聚体的形成和微生物的生长。

关键词: 土壤团聚体, 生物炭, 腐植酸, 微生物群落, 丹江口库区

CLC Number:

YU Jingjing, CAI Debao, CHEN Xiuwen, ZHANG Jun, CHEN Jibao. Effect of Biochar and Humic Acid Addition on the Soil Aggregate of Danjiangkou Reservoir[J]. Journal of Henan Agricultural Sciences, 2021, 50(11): 87-96.

于静静, 蔡德宝, 陈秀文, 张君, 陈吉宝. 生物炭和腐植酸对丹江口库区土壤团聚体的影响[J]. 河南农业科学, 2021, 50(11): 87-96.

Figures/Tables 5

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