Effect of PGPR and Melatonin on the Antioxidant Properties and Arsenic Absorption of Coreopsis grandiflora

doi:10.15933/j.cnki.1004-3268.2025.03.010

Abstract

Abstract: Using Chaoyang seedlings of Coreopsis grandiflora as test materials，an indoor pot experiment was conducted to investigate the effects of inoculating plant growth promoting bacteria（PGPR）and foliar spraying of melatonin（MT）at concentrations of 5 μmol/L（MT₁），20 μmol/L（MT₂），and 50 μmol/L（MT₃）under 80 mg/kg arsenic（As） stress on the growth，root architecture，chlorophyll content，chlorophyll fluorescence parameters，nutrient elements，As absorption，and antioxidant properties of C.grandiflora.The results showed that compared with the CK treatment without As stress，the overall plant height，leaf area，and total biomass（aboveground and root dry weight）of C.grandiflora were significantly reduced under As stress.Foliar spraying of different concentrations of MT and MT+PGPR treatment could alleviate the damage of As stress to C.grandiflora. Under As stress，compared with the treatment without PGPR inoculation or MT spraying，the root system configuration（total root length，total volume，total surface area，root tip number）of C.grandiflora treated with As+MT2+PGPR was significantly improved.The root activity increased by 166.1%，and the chlorophyll content，maximum photochemical efficiency（Fv/Fm） of PS Ⅱ ，and photochemical quenching coefficient（qP） increased by 56.7%，15.6%，and 62.8%，respectively，while the non photochemical quenching coefficient（NPQ）decreased by 23.7%.As stress significantly reduced the nitrogen，phosphorus，and potassium content in the aboveground and root systems of C.grandiflora，while As+MT2+PGPR treatment could increase the nitrogen，phosphorus，and potassium content in the aboveground and root systems to varying degrees.At the same time，foliar spraying of different concentrations of MT and MT+PGPR could increase the As content in the aboveground and root parts of C. grandiflora，improve the As transport coefficient and enrichment coefficient，activate the activities of superoxide dismutase（SOD），catalase（CAT），ascorbate peroxidase（APX），and glutathione reductase（GR），and increase the content of ascorbic acid（AsA）and glutathione（GSH）to alleviate oxidative stress on C.grandiflora.In summary，As+MT₂+PGPR treatment has the best effect on improving the resistance of C.grandiflora to As pollution.

Key words: Coreopsis grandiflora, Plant growth promoting rhizobacteria, Melatonin, Arsenic stress, Root architecture, Antioxidant enzyme

摘要： 以大花金鸡菊为试材，采用室内盆栽试验，探究80 mg/kg砷（As）胁迫下接种植物根际促生菌（PGPR）与叶面喷施5 μmol/L（MT₁）、20 μmol/L（MT₂）、50 μmol/L（MT₃）褪黑素（MT）及其复合处理对大花金鸡菊生长、根系构型、叶绿素含量及叶绿素荧光参数、营养元素、As吸收及抗氧化特性的影响。结果表明，与无As胁迫的CK处理相比，As胁迫下大花金鸡菊株高、叶面积、总生物量（地上部和根系干质量）整体上显著降低。叶面喷施不同浓度MT和MT+PGPR处理可以减轻As胁迫对大花金鸡菊的伤害。As胁迫下，与不接种PGPR不喷施MT处理相比，As+MT₂+PGPR处理的大花金鸡菊根系构型（总根长、总体积、总表面积、根尖数）显著改善，根系活力提高了166.1%，叶绿素含量、PSⅡ最大光化学效率（Fv/Fm）、光化学淬灭系数（qP）分别增加56.7%、15.6%和62.8%，而非光化学淬灭系数（NPQ）下降了23.7%。As胁迫显著降低了大花金鸡菊地上部和根系中氮、磷、钾含量，As+MT₂+PGPR处理能不同程度地增加地上部和根系中氮、磷、钾含量。同时，叶面喷施不同浓度MT和MT+PGPR处理能够增加大花金鸡菊地上部和根系As含量，提高大花金鸡菊As转运系数和富集系数，激活超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、抗坏血酸过氧化物酶（APX）和谷胱甘肽还原酶（GR）活性并增加抗坏血酸（AsA）和谷胱甘肽（GSH）含量，从而缓解大花金鸡菊受到的氧化胁迫。综上，As+MT₂+PGPR处理提高大花金鸡菊抵抗As污染的效果最好。

关键词: 大花金鸡菊, 植物根际促生菌, 褪黑素, 砷胁迫, 根系构型, 抗氧化酶

CLC Number:

S682

LIANG Ya’nan, WU Mengxi, XIAO Yongqin, ZENG Jingjin, DUAN Yili. Effect of PGPR and Melatonin on the Antioxidant Properties and Arsenic Absorption of Coreopsis grandiflora[J]. Journal of Henan Agricultural Sciences, 2025, 54(3): 87-98.

梁亚男, 邬梦晞, 肖雍琴, 曾鲸津, 段益莉. 植物根际促生菌和褪黑素对大花金鸡菊抗氧化特性和砷吸收的影响[J]. 河南农业科学, 2025, 54(3): 87-98.

Figures/Tables 6

References

［1］GOYAL V C，SINGH O，SINGH R，et al. Appraisal of heavy metal pollution in the water resources of Western Uttar Pradesh， India and associated risks［J］.Environmental Advances，2022，8：100230.

［2］NIAZI N K，HUSSAIN M M，BIBI I，et al. The significance of eighteen rice genotypes on arsenic accumulation， physiological response and potential health risk［J］. Science of the Total Environment，2022，832：155004.

［3］储建民，邱文杰，吴剑锋，等. 固废填埋场地土壤-地下水的砷污染反应运移模拟与源强识别［J］. 环境科学学报，2024，44（2）：237⁃247.
CHU J M，QIU W J，WU J F，et al. Simulation of arsenic pollution reactive transport and source intensity identification of soil⁃groundwater in a solid waste landfill site［J］.Acta Scientiae Circumstantiae，2024，44（2）：237⁃247.

［4］ZANDSALIMI S，ABDI C，BAHMANI O. Study of arsenic in water and soil sources of a contaminated region and its absorption and accumulation rate in the local pasture plants of the area（Case study：Bijar City）［J］. Journal of Water and Soil Conservation，2024，31 （1）：73.

［5］马强，卫泽斌，吴启堂. 化学淋洗联合电动技术修复重金属污染土壤的效果及其机制［J］. 环境科学，2023，44 （3）：1668⁃1677.
MA Q，WEI Z B，WU Q T. Effectiveness and mechanisms of chemical leaching combined with electrokinetic technology on the remediation of heavy metal contaminated soil［J］. Environmental Science，2023，44（3）：1668⁃1677.

［6］LI L J，TIAN J，HUANG K，et al. Metal⁃binding protein TaGlo1 improves fungal resistance to arsenite（As^Ⅲ）and methylarsenite（MAs^Ⅲ）in paddy soil［J］. Environmental Science & Technology，2024，58（17）：7469⁃7479. ［7］LI C，DING S M，MA X，et al. Sediment arsenic remediation by submerged macrophytes via root⁃released O₂ and microbe⁃mediated arsenic biotransformation［J］.Journal of Hazardous Materials，2023，449：131006.

［8］马莹，王玥，石孝均，等. 植物促生菌在重金属生物修复中的作用机制及应用［J］.环境科学，2022，43（9）：4911⁃4922.
MA Y，WANG Y，SHI X J，et al. Mechanism and application of plant growth⁃promoting bacteria in heavy metal bioremediation［J］. Environmental Science，2022，43（9）：4911⁃4922.

［9］ MANOJ S R，KARTHIK C，KADIRVELU K，et al.Understanding the molecular mechanisms for the enhanced phytoremediation of heavy metals through plant growth promoting rhizobacteria：A review［J］.Journal of Environmental Management，2020，254：109779.

［10］SILAMBARASAN S，LOGESWARI P，CORNEJO P，et al. Role of plant growth⁃promoting rhizobacterial consortium in improving the Vigna radiata growth and alleviation of aluminum and drought stresses［J］.Environmental Science and Pollution Research International，2019，26（27）：27647⁃27659.

［11］伍国强，于祖隆，魏明. PGPR调控植物响应逆境胁迫的作用机制［J］. 草业学报，2024，33（6）：203⁃218.
WU G Q，YU Z L，WEI M. The mechanism of PGPR regulating plant response to abiotic stress［J］. Acta Prataculturae Sinica，2024，33（6）：203⁃218.

［12］AGARWAL H，DOWARAH B，BARUAH P M，et al.Endophytes from Gnetum gnemon L.can protect seedlings against the infection of phytopathogenic bacterium Ralstonia solanacearum as well as promote plant growth in tomato［J］. Microbiological Research，2020，238：126503.

［13］陈生涛，何琳燕，李娅，等. Rhizobium sp. W33对不同植物吸收铜和根际分泌物的影响［J］. 环境科学学报，2014，34（8）：2077⁃2084.
CHEN S T，HE L Y，LI Y，et al. Effect of Rhizobium sp. W33 on copper accumulation and organic exudations of different plants grown on copper⁃contaminated soil［J］. Acta Scientiae Circumstantiae，2014，34（8）：2077⁃2084.

［14］MA Y，RAJKUMAR M，MORENO A，et al. Serpentine endophytic bacterium Pseudomonas azotoformans ASS1 accelerates phytoremediation of soil metals under drought stress［J］. Chemosphere，2017，185：75⁃85.

［15］李旖曦，陈涵冰，王耀强，等. 耐砷促生菌对超富集植物蜈蚣草砷吸收及根际微生物群落的调控作用［J］.微生物学报，2023，63（6）：2401⁃2414.
LI Y X，CHEN H B，WANG Y Q，et al.Effects of arsenic⁃tolerant growth⁃promoting bacteria on arsenic uptake and rhizosphere microbial community of hyperaccumulator Pteris vittata［J］. Acta Microbiologica Sinica，2023，63（6）：2401⁃2414.

［16］李圆圆，张文静，李楠. 砷胁迫下接种PGPR对水生植物砷累积及生理特性的影响［J］.河南农业科学，2024，53（6）：67⁃78.
LI Y Y，ZHANG W J，LI N. Effects of PGPR on arsenic accumulation and physiological characteristics of aquatic plants under arsenic stress［J］. Journal of Henan Agricultural Sciences，2024，53（6）：67⁃78.

［17］杨过，聂圣松，杭俊楠，等. 褪黑素在植物生长发育和逆境响应中的研究进展［J］.山地农业生物学报，2022，41（6）：37⁃46.
YANG G，NIE S S，HANG J N，et al. Research progress of melatonin in plant growth and development and stress response［J］. Journal of Mountain Agriculture and Biology，2022，41（6）：37⁃46.

［18］徐灵，冯艳，王秋洁，等. 外源褪黑素对铬胁迫下马铃薯生长、铬耐受及土壤降铬的影响［J］.南方农业学报，2024，55（11）：3275⁃3285.
XU L，FENG Y，WANG Q J，et al. Effects of exogenous melatonin on potato growth，chromium tolerance， and soil chromium reduction under chromium stress［J］. Journal of Southern Agriculture，2024，55（11）：3275⁃3285.

［19］黄益宗，蒋航，王农，等. 外源褪黑素对砷胁迫下水稻幼苗生长的影响［J］. 生态学杂志，2018，37（6）：1738⁃1743.
HUANG Y Z，JIANG H，WANG N，et al. Effects of exogenous melatonin on the growth of rice seedlings under As stress［J］. Chinese Journal of Ecology，2018，37（6）：1738⁃1743.

［20］张盛楠，黄亦玟，陈世宝，等. 不同外源物质对镉砷复合污染胁迫下油菜生理指标和镉砷积累的影响［J］. 生态学杂志，2020，39（7）：2214⁃2222.
ZHANG S N，HUANG Y M，CHEN S B，et al. Effects of spraying different exogenous substances on physiological indices and cadmium and arsenic accumulation in rape under combined pollution of cadmium and arsenic［J］.Chinese Journal of Ecology，2020，39（7）：2214⁃2222.

［21］BHARDWAJ T，KOUR J，CHOUHAN R，et al.Integrated transcriptomic and physio⁃molecular studies unveil the melatonin and PGPR induced protection to photosynthetic attributes in Brassica juncea L. under cadmium toxicity［J］. Journal of Hazardous Materials，2024，476：134875.

［22］蒋航. 外源褪黑素对砷胁迫下水稻萌发及幼苗生长的影响［D］. 武汉：华中农业大学，2017.
JIANG H. Effects of exogenous melatonin on rice germination and seedling growth under arsenic stress［D］.Wuhan：Huazhong Agricultural University，2017.

［23］邱丹，杜芮萍，孟德凯，等.玉米套作蜈蚣草修复砷污染农田土壤的效应研究［J］. 农业环境科学学报，2017，36（1）：101⁃107.
QIU D，DU R P，MENG D K，et al. Effect of maize（Pteris vittata L.） intercropping on remediation of As⁃contaminated farmland soil ［J］. Journal of Agro⁃Environment Science，2017，36（1）：101⁃107.

［24］刘映彤，薛林贵，何园园，等.土壤高耐砷促生菌株的筛选及其对砷去除性能的研究［J］.兰州交通大学学报，2022，41（2）：118⁃127.
LIU Y T，XUE L G，HE Y Y，et al. Screening of soil high arsenic⁃tolerant growth⁃promoting strains and their arsenic removal performance［J］.Journal of Lanzhou Jiaotong University，2022，41（2）：118⁃127.

［25］LI X，LI B Q，ZHENG Y，et al. Physiological and rhizospheric response characteristics to cadmium of a newly identified cadmium accumulator Coreopsis grandiflora Hogg.（Asteraceae）［J］. Ecotoxicology and Environmental Safety，2022，241：113739.

［26］刘云朝.外源褪黑素对铅胁迫下狗牙根生长及抗氧化性的影响［J］.中国草地学报，2024，46（1）：97⁃107.
LIU Y Z. Effects of exogenous melatonin on the growth and antioxidant of bermudagrass under lead stress［J］.Chinese Journal of Grassland，2024，46（1）：97⁃107.

［27］白宝璋，金锦子，白崧，等. 玉米根系活力TTC测定法的改良［J］.玉米科学，1994，2（4）：44⁃47.
BAI B Z，JIN J Z，BAI S，et al. Improvement of TTC method determining root activity in corn［J］.Journal of Maize Sciences，1994，2（4）：44⁃47.

［28］李忠光，杜朝昆，龚明. 在单一提取系统中同时测定植物ASA/DHA和GSH/GSSG［J］. 云南师范大学学报（自然科学版），2003，23（3）：67⁃70.
LI Z G，DU C K，GONG M. Simultaneous measurement of ASA/DHA and GSH/GSSG using a single extraction system［J］. Journal of Yunnan Normal University（Natural Sciences Edition），2003，23（3）：67⁃70.

［29］刘虎鹏，张云慧，袁贝，等. 我国污染地块土壤砷生物可给性关键影响因素研究［J］. 环境科学研究，2024，37（9）：1986⁃1996.
LIU H P，ZHANG Y H，YUAN B，et al. Study on key factors affecting arsenic bioaccessbility in contaminated sites in China［J］. Research of Environmental Sciences，2024，37（9）：1986⁃1996.

［30］CHAUHAN R，AWASTHI S，TIWARI P，et al.Biotechnological strategies for remediation of arsenic⁃contaminated soils to improve soil health and sustainable agriculture［J］. Soil & Environmental Health，2024，2（1）：100061.

［31］ LI Y，CHENG L，YANG B L，et al.Remediation of Cd⁃As⁃Ni co⁃contaminated soil by extracellular polymeric substances from Bacillus subtilis：Dynamic improvements of soil properties and ecotoxicity［J］.Science of the Total Environment，2024，955：177009.

［32］吴萍民，冷艳，程斌，等.外源ABA对镉胁迫下绿豆幼苗生长及内源激素的影响［J］.环境科学学报，2023，43（7）：391⁃400.
WU P M，LENG Y，CHENG B，et al.Effects of exogenous ABA on the growth and contents of endogenous hormones in mung bean seedlings under cadmium stress［J］.Acta Scientiae Circumstantiae，2023，43（7）：391⁃400.

［33］许天宇，夏涛，王晓菡. 植物促生细菌阻控小麦吸收镉和砷的效应研究［J］.齐鲁工业大学学报，2023，37（2）：52⁃60.
XU T Y，XIA T，WANG X H. Effects of plant growth⁃promoting bacteria on inhibiting the uptake of cadmium and arsenic by wheat［J］. Journal of Qilu University of Technology，2023，37（2）：52⁃60.

［34］姚欢，王铤，刘磊，等. 褪黑素对大头菜幼苗生长及镉积累的影响［J］. 土壤，2019，51（5）：1001⁃1005.
YAO H，WANG T，LIU L，et al. Effects of melatonin on growth and cadmium accumulation of Brassica juncea var.megarrhiza［J］.Soils，2019，51（5）：1001⁃1005.

［35］CHEN Z P，GU Q，YU X L，et al. Hydrogen peroxide acts downstream of melatonin to induce lateral root formation［J］. Annals of Botany，2018，121（6）：1127⁃1136.

［36］李亮，辛在军，王玺洋，等. 褪黑素对镉胁迫下紫苏根系特征的影响［J］. 农业环境科学学报，2022，41（2）：257⁃266.
LI L，XIN Z J，WANG X Y，et al. Effects of exogenous melatonin on root characteristics of Perilla frutescens under cadmium stress［J］. Journal of Agro⁃Environment Science，2022，41（2）：257⁃266.

［37］KUMAR A， TRIPTI， VOROPAEVA O， et al. Bioaugmentation with copper tolerant endophyte Pseudomonas lurida strain EOO26 for improved plant growth and copper phytoremediation by Helianthus annuus［J］.Chemosphere，2021，266：128983.

［38］王苗. 植物促生菌强化美洲商陆（Phytolacca americana L.）修复重金属污染土壤及对根际微生物群落影响的研究［D］. 南京：南京农业大学，2021.
WANG M. Study on Phytolacca americana L.remediation of heavy metal contaminated soil by plant growth⁃promoting bacteria and its influence on rhizosphere microbial community［D］. Nanjing：Nanjing
Agricultural University，2021.

［39］SOBARIU D L，FERTU D I T，DIACONU M，et al.Rhizobacteria and plant symbiosis in heavy metal uptake and its implications for soil bioremediation［J］.New Biotechnology，2017，39：125⁃134.

［40］SAHA M，MAURYA B R，MEENA V S，et al.Identification and characterization of potassium solubilizing bacteria（KSB）from Indo⁃Gangetic Plains of India ［J］. Biocatalysis and Agricultural Biotechnology，2016，7：202⁃209.

［41］龚明贵，刘凯洋，魏亚楠，等. 砷胁迫下接种丛枝菌根真菌对棉花光合特性和叶肉细胞超微结构的影响［J］.棉花学报，2022，34（3）：256⁃266.
GONG M G，LIU K Y，WEI Y N，et al. Effects of arbuscular mycorrhizal fungi on photosynthetic characteristics and mesophyll cell ultrastructure of cotton under arsenic stress［J］.Cotton Science，2022，34（3）：256⁃266.

［42］魏婷，高菡，杨代忠，等.根际促生菌对镉胁迫下油菜生理生化及品质的影响［J］.陕西科技大学学报，2023，41（4）：1⁃7.
WEI T，GAO H，YANG D Z，et al.Effects of plant growth⁃promoting rhizobacteria on physiological properties and quality of pakchoil plants under cadmium stress［J］. Journal of Shaanxi University of Science & Technology，2023，41（4）：1⁃7.

［43］MIRZAHOSSINI Z，SHABANI L，SABZALIAN M R，et al.ABC transporter and metallothionein expression affected by NI and Epichloe endophyte infection in tall fescue［J］. Ecotoxicology and Environmental Safety，2015，120：13⁃19.

［44］ETESAMI H，MAHESHWARI D K. Use of plant growth promoting rhizobacteria（PGPRs）with multiple plant growth promoting traits in stress agriculture：Action mechanisms and future prospects ［J］.Ecotoxicology and Environmental Safety，2018，156：225⁃246.

［45］孙传蛟，谭清文，刘晓雪，等. 外源褪黑素对铬（Cr6+）胁迫下小麦幼苗光合特性和养分吸收的影响［J］. 麦类
作物学报，2022，42（12）：1535⁃1542.
SUN C J，TAN Q W，LIU X X，et al. Effects of exogenous melatonin on photosynthetic characteristics and nutrient absorption of wheat seedlings under Cr6+ stress［J］. Journal of Triticeae Crops，2022，42 （12）：1535⁃1542.

［46］沈甜，王琼瑶，崔永亮，等. 植物根际促生细菌对蒲儿根富集铜及土壤理化性质的影响［J］. 农业环境科学学报，2020，39（3）：572⁃580.
SHEN T，WANG Q Y，CUI Y L，et al. Effects of plant growth⁃promoting rhizobacteria on the copper enrichment ability of Sinosenecio oldhamianus and physicochemical properties of soil［J］. Journal of Agro⁃Environment Science，2020，39（3）：572⁃580.
［47］李英军. 植物促生细菌SR-9提高甜高粱富集镉的效应及机理研究［D］.南阳：南阳师范学院，2022.

LI YJ. Effect and mechanism of plant growth⁃promoting bacteria SR⁃9 on cadmium enrichment in sweet Sorghum［D］.Nanyang：Nanyang Normal University，2022.
［48］SALEEM M H，FAHAD S，KHAN S U，et al.Copper⁃induced oxidative stress， initiation of antioxidants and phytoremediation potential of flax（Linum usitatissimum L. ）seedlings grown under the mixing of two different soils of China［J］.Environmental Science and Pollution Research International，2020，27（5）：5211⁃5221.
［49］林小兵，周利军，吴多基，等. 镉胁迫对粉葛幼苗生长、生理特性及镉富集的影响［J］. 江西农业大学学报，2024，46（4）：884⁃893.
LIN X B，ZHOU L J，WU D J，et al. Effect of cadmium stress on growth，physiological characteristics and
cadmium enrichment of Pueraria thomsonii Benth.seedlings ［J］. Acta Agriculturae Universitatis Jiangxiensis，2024，46（4）：884⁃893.
［50］崔宏莉，解静芳，杨彪，等. 污灌与镉胁迫对菠菜几种抗氧化酶活性的影响［J］.生态毒理学报，2010，5（2）：
274⁃279.
CUI H L，XIE J F，YANG B，et al. Effects of sewage irrigation and cadmium stresses on the activities of several antioxidant enzymes of spinach［J］.Asian Journal of Ecotoxicology，2010，5（2）：274⁃279.
［51］HUSNA，HUSSAIN A，SHAH M，et al. Phytohormones producing rhizobacteria alleviate heavy metals stress in soybean through multilayered response［J］.Microbiological Research，2023，266：127237.
［52］艾金祥，宋嘉怡，严浙楠，等.褪黑素对铅胁迫下虎舌红和朱砂根生理响应及DNA损伤的调控效应［J］. 植物学报，2022，57（2）：171⁃181.
AI J X，SONG J Y，YAN Z N，et al. Effects of exogenous melatonin on physiological response and DNA damage of Ardisia mamillata and A.crenata under lead stress［J］. Chinese Bulletin of Botany，2022，57（2）：171⁃181.

[1]	TIAN Yunfang, CHU Zhigang, CHEN Lipei, HE Yixin, LIU Xiaojuan, FAN Chunli. Analysis on the Preservation Effect of Melatonin on Cut Chrysanthemum [J]. Journal of Henan Agricultural Sciences, 2025, 54(1): 119-127.
[2]	YANG Yan, XIAO Bin. Effects of Exogenous Melatonin on the Photosynthesis，ASA‑GSH Cycle，and Hormone Changes of Malus‘Royalty’under Drought Stress [J]. Journal of Henan Agricultural Sciences, 2024, 53(6): 100-110.
[3]	MA Zhonglian, GONG jianfu, MA Yongcui, TIAN Hong, LIU Defu, YANG Shunqiang. Effects of Exogenous Melatonin on Physiological Characters，Yield and Quality of Tartary Buckwheat [J]. Journal of Henan Agricultural Sciences, 2024, 53(1): 41-47.
[4]	LIANG Dong, LIU Guangliang, SHI Yi, WANG Shusheng, WANG Chengdong, WANG Xiaolin. Effect of Brassinolide on Root Architecture of Tobacco Seedlings and Regulation Mechanism [J]. Journal of Henan Agricultural Sciences, 2022, 51(8): 37-44.
[5]	HE Jianqing, ZHANG Gejie. Effects of Plant Growth Promoting Rhizobacteria（PGPR）Fertilizer instead of Part of Chemical Fertilizer on Growth，Yield and Quality of Black Highland Barley [J]. Journal of Henan Agricultural Sciences, 2022, 51(7): 93-101.
[6]	CUI Zhengguo, CAO Qingjun, HU Bo, WANG jingyu, YANG Hao, LI Gang. Effect of Different Priming Treatments on Seed Germination and Seedling Growth of Waxy Maize under Low Temperature Stress [J]. Journal of Henan Agricultural Sciences, 2022, 51(3): 47-54.
[7]	SUN Haoyue, WU Hongbin, LI Ming, ZHANG Qi, HAN Yiqiang, DU Jidao. Effects of Melatonin Seed Soaking on Growth and Physiological Characteristics of Kidney Bean Seedlings under Salt Stress [J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 111-120.
[8]	ZHAO Yanxia, WU Xinying, WANG Pengfei, QI Guimei. Research Progress of Melatonin Application in Grapes [J]. Journal of Henan Agricultural Sciences, 2020, 49(8): 1-5.
[9]	LI Yilun, ZHANG Yanyan, YU Hao, YU Hui, YIN Quanyu, LI Benyin, LI Xuanzhen, LI Junpeng, WU Mingzuo. Effects of Plant Growth Promoting Rhizobacteria on Tobacco Uptake and Accumulation of Cd [J]. Journal of Henan Agricultural Sciences, 2020, 49(4): 62-71.
[10]	SHEN Yanchao, ZHANG Zijing, YANG Guofeng, ZHANG Shikun, WANG Eryao, WANG Yinggang, HUANG Yongzhen, WANG Xianwei, BAI Xianxiao, XU Zhaoxue, WANG Sanhu, ZHANG Wei. Research Progress on the Role of Melatonin in Male Animal Reproduction [J]. Journal of Henan Agricultural Sciences, 2020, 49(3): 1-7.
[11]	LI Hongjie. Effects of Exogenous Melatonin and Silicon on the Growth and Physiological Characteristics of Celery(Apium graveolens) Seedlings under Salt Stress [J]. Journal of Henan Agricultural Sciences, 2020, 49(1): 96-102.