Abstract: In order to explore the effects of different priming treatments on low temperature resistance of waxy maize，Wannuo 2000 was used as material，early sowing was adopted in field，and the effects of seed priming with 0.5 mmol/L melatonin（MT），0.5 mmol/L spermidine（Spd），and 0.5 mmol/L MT + 0.5 mmol/L Spd（MT+Spd）on seed germination and seedling growth of waxy maize were studied under low temperature stress. The results showed that compared with the control without priming agent，the emergence rates of priming treatments（MT，Spd and MT + Spd）significantly increased by 26.05，27.09 and 31.25 percent points respectively，the emergence time significantly reduced by 3.75、4.50、5.25 days，the shoot height and uniformity of shoot height also significantly improved under low temperature stress，and MT+Spd treatment had the best effect. Meanwhile，priming treatment significantly increased the root surface area，root length，root diameter，dry weight of shoot，dry weight of root and root⁃shoot ratio of waxy maize seedling under low temperature stress，and MT+Spd treatment had the best effect.Correlation analysis showed that there were significantly positive correlations between dry weight of shoot and dry weight of root，root surface area，as well as root length.Thus，seed priming is a feasible way to enhance low temperature resistance under field condition，and MT+Spd treatment has the best effect.

Key words: Waxy maize, Low temperature stress, Seed priming, Melatonin（MT）, Spermidine（Spd）

摘要： 为了探讨不同引发处理对糯玉米低温抗性的影响，以糯玉米万糯2000为试验材料，采用大田早播的方法研究0.5 mmol/L褪黑素（MT）、0.5 mmol/L亚精胺（Spd）以及0.5 mmol/L褪黑素+0.5 mmol/L亚精胺（MT+Spd）3种引发处理对低温下糯玉米种子萌发与幼苗生长的影响。结果表明，在低温条件下，引发处理（MT、Spd、MT + Spd）糯玉米出苗率分别较无引发剂处理（CK）显著提高26.05、27.09、31.25个百分点，种子出苗时间分别较CK显著缩短3.75、4.50、5.25 d，株高和株高整齐度均较CK显著提高，以MT+Spd处理效果最明显。引发处理均能显著增加低温下糯玉米根系表面积、根长、根系直径、地上部干质量、根系干质量、根冠比等，以MT+Spd处理效果最明显。相关分析结果表明，地上部干质量与根系干质量、根系表面积、根长均呈显著正相关。综合分析，利用引发处理提高大田条件下种子低温抗性是可行的，以MT+Spd处理效果最好。

关键词: 糯玉米, 低温胁迫, 种子引发, 褪黑素, 亚精胺