Journal of Henan Agricultural Sciences ›› 2021, Vol. 50 ›› Issue (8): 94-102.DOI: 10.15933/j.cnki.1004-3268.2021.08.012

Special Issue: 拮抗菌的筛选专题

• Plant Protection • Previous Articles     Next Articles

Screening and Control Effect of Endophytic Fungi for Brown Spot Disease of Schisandra chinensis

ZHANG Yuqi1,REN Yueying1,WEN Xiangsui1,WANG Yumeng1,FANG Ping1,LI Baoquan2,LENG Shihe3   

  1. (1.College of Herbal Medicine,Jilin Agricultural University,Changchun 130118,China;2.Ji’an Emperor Valley Schisandra Planting Professional Cooperative,Tonghua 134200,China;3.Baishan Jiahe Specialty Co.,Ltd.,Baishan 134300,China)
  • Received:2020-10-18 Published:2021-08-15 Online:2021-09-17

北五味子褐斑病内生生防真菌筛选及防治效果研究

张誉荠1,任跃英1,文湘穗1,王雨濛1,方平1,李宝全2,冷世和3   

  1. (1.吉林农业大学中药材学院,吉林长春130118;2.集安帝王谷北五味子种植专业合作社,吉林通化134200;3.白山市嘉和特产有限公司,吉林白山134300)
  • 通讯作者: 任跃英(1958-),女,吉林长春人,教授,博士,主要从事药用植物栽培及育种研究。E-mail:381717169@qq.com
  • 作者简介:张誉荠(1996-),男,云南昆明人,在读硕士研究生,研究方向:药用植物生物防治。E-mail:906029625@qq.com
  • 基金资助:
    吉林省科技发展计划基金项目(20170307032YY)

Abstract: In order to screen the excellent endophytic fungi with better biocontrol effects on the pathogen of Schisandra chinensis brown spot disease,Phoma glomerate(corad)Wollena ex Hoehapf,the conventional culture method in plant pathology was used to culture Phoma glomerate(corad)Wollena ex Hoehapf and observe its colony characteristics and micromorphology,at the same time,the plate antagonistic method was applied to screen the endophytic biocontrol fungi. By measuring the change of cell membrane permeability,soluble protein content,ergosterol content,and chitin content of Phoma glomerate(corad)Wollena ex Hoehapf,the bactericidal mechanism of WG9 fermentation liquid was preliminarily explored. Morphological observation,fungal ITS gene sequencing,and the construction of phylogenetic tree were combined to identify the screened excellent endophytic biocontrol fungi.The results showed that one strain of biocontrol fungi WG9 screened from the endophytic fungi of Schisandra chinensis had better biocontrol effect. The plate antagonistic experiment indicated that its antagonistic rate reached 64. 73% and the antagonistic rate of fermentation liquid was 48. 21%. With the increase of the concentration of the WG9 fermentation broth,the cell membrane permeability and soluble protein content of the pathogenic fungi hyphae increased,while the ergosterol content and chitin content decreased. Morphological observation and molecular biology identified the excellent endophytic fungus as Purpureocillium lilacinum. The present study showed that the endophytic biocontrol fungus WG9 screened from the rhizome of Schisandra chinensis had a good bactericidal effect on Phoma glomerate(corad)Wollena ex Hoehapf,and the WG9 fermentation broth had a certain damage effect on the cell wall of pathogenic fungi.

Key words: Schisandra chinensis(Tuecz.)Baill., Brown spot, Biological control, Endophytic fungi, Purpureocillium lilacinum

摘要: 为筛选对北五味子褐斑病病原菌[Phoma glomerate(corad)Wollena ex Hoehapf]生防效果较好的优良内生真菌,采用植物病理学常规培养法对北五味子褐斑病病原菌进行培养并对菌落特征及微观形态进行观察,同时利用平板对峙法对内生真菌进行筛选,通过测定褐斑病病原菌的细胞膜透性和可溶性蛋白、麦角甾醇、几丁质含量变化,初步探究WG9发酵液的抑菌机制;结合形态学观察、真菌ITS基因测序及构建系统发育树的方法对筛选出的优良内生生防真菌进行鉴定。结果表明,从北五味子内生真菌中筛选出1株生防效果较好的生防真菌WG9,平板对峙试验显示其拮抗率达64.73%,发酵液拮抗率为48.21%;随着WG9发酵液浓度的增加,病原菌菌丝的细胞膜透性和可溶性蛋白含量升高,麦角甾醇和几丁质含量降低;经形态学观察及分子生物学鉴定,该优良内生生防真菌为淡紫拟青霉(Purpureocillium lilacinum)。研究表明,从北五味子根茎中筛选出的内生生防真菌WG9对褐斑病病原菌有较好的抑制作用,WG9发酵液对病原菌的细胞壁有一定的损伤作用。

关键词: 北五味子, 褐斑病, 生物防治, 内生真菌, 淡紫拟青霉

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