Isolation,Identification and Genetic Characterization of Goose Astrovirus Isolate XX

doi:10.15933/j.cnki.1004-3268.2021.06.016

Journal of Henan Agricultural Sciences ›› 2021, Vol. 50 ›› Issue (6): 134-141.DOI: 10.15933/j.cnki.1004-3268.2021.06.016

• Animal Husbandry and Veterinary Medicine • Previous Articles Next Articles

Isolation,Identification and Genetic Characterization of Goose Astrovirus Isolate XX

JIN Qianyue^1,2,3,GUO Yonggang⁴,LI Junpeng⁵,QIN Baoliang⁶,WANG Yinbiao⁷,GUO Zhenhua^1,2,3,WANG Li^1,2,3,XING Guangxu^1,2,3,DENG Ruiguang^1,2,3,ZHANG Gaiping^1,2,3,8

(1.Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.UK-China Centre of Excellence for Research on Avian Diseases,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;3.Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou 225009,China; 4.College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China; 5.Animal Husbandry Research Center,Pingdingshan Academy of Agricultural Sciences,Pingdingshan467001,China; 6.Xinxiang Center for Animal Disease Control and Prevention,Xinxiang 453003,China; 7.School of Public Health,Xinxiang Medical University,Xinxiang 453003,China; 8.College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China)

Received:2020-12-22 Published:2021-06-15 Online:2021-06-15

鹅星状病毒XX株的分离鉴定及遗传特征分析

金前跃^1,2,3，郭永刚⁴，李俊朋⁵，秦保亮⁶，王寅彪⁷，郭振华^1,2,3，王丽^1,2,3，邢广旭^1,2,3，邓瑞广^1,2,3，张改平^1,2,3,8

（1.河南省农业科学院动物免疫学重点实验室，河南郑州 450002；2.河南省农业科学院中英禽病国际研究中心，河南郑州 450002；3.江苏高校动物重要疾病与人兽共患病防控协同创新中心，江苏扬州 225009；4.河南牧业经济学院动物医药学院，河南郑州 450046；5.平顶山市农业科学院畜牧研究中心，河南平顶山 467001；6.新乡市动物疫病预防控制中心，河南新乡 453003；7.新乡医学院公共卫生学院，河南新乡 453003；8.河南农业大学动物医学院，河南郑州 450002）

通讯作者: 张改平（1960-），男，河南内黄人，中国工程院院士，研究员，博士，主要从事动物免疫学研究。E-mail:zhanggaip@126.com
作者简介:金前跃（1982-），男，浙江建德人，助理研究员，博士，主要从事动物疫病的监测与防控。E-mail:1956089861@qq.com
基金资助:
国家重点研发计划重点专项（2016YFD0500800）；河南省重点研发与推广专项（科技攻关）（202102110100，202102110248）；农业部动物疫病观测监测项目（ZX06S1701）

Abstract

Abstract: In order to study the genomic characteristics of goose astrovirus (GAstV) causing gosling gout in Henan Province,a strain of GAstV was isolated from goose samples with gout,named XX strain,and analysis of whole genome sequence and genetic characteristics of the virus was performed.The results showed that GAstV XX strain could be stably passaged on LMH cells without obvious cytopathic effect.The full length of the genome of the GAstV XX strain was 7 252 bp, and the nucleotide sequence homology was 98.1%,98.7% and 98.7% compared with GD,AstV/SDPY/Goose/1116/17 and AAstV/Goose/CHN/2017/ SD01,respectively. Phylogenetic analysis showed that GAstV XX strain was in the same evolutionary branch as GD, AstV/ SDPY/ Goose/ 1116/ 17 and AAstV/ Goose/ CHN/ 2017/ SD01, belonging to group 1 of avian astrovirus.The amino acid sequence analysis of ORF2 encoded protein showed that there were multiple amino acid mutations in the isolated GAstV XX strain compared with the reported epidemic strains.

Key words: Goose astrovirus, Isolation and identification, Whole genome sequencing, Genetic characterization, Homology, Phylogenetic analysis

摘要： 为研究引起雏鹅痛风疫情的鹅星状病毒（Goose astrovirus，GAstV）河南流行株的基因组特征，从患有痛风的雏鹅样品中分离了1株GAstV，命名为XX株，并对分离毒株进行了全基因组测序和遗传特征分析。结果显示，GAstV XX株可在LMH细胞上稳定传代，但无明显的细胞病变；该毒株基因组全长7 252 bp，与代表性毒株GD、AstV/SDPY/Goose/1116/17、AAstV/Goose/CHN/2017/SD01的基因组核苷酸序列同源性分别为98.1%、98.7%、98.7%。系统进化分析结果显示，GAstV XX株与GD、AstV/SDPY/Goose/1116/17、AAstV/Goose/CHN/2017/SD01等毒株处于同一进化分支，属于禽星状病毒1群。ORF2编码蛋白氨基酸序列分析结果显示，与已报道的GAstV流行毒株相比，GAstV XX株存在多个氨基酸位点的突变。

关键词: 鹅星状病毒, 分离鉴定, 全基因组测序, 遗传特征, 同源性, 系统进化分析

CLC Number:

S852.65

JIN Qianyue, GUO Yonggang, LI Junpeng, QIN Baoliang, WANG Yinbiao, GUO Zhenhua, WANG Li, XING Guangxu, DENG Ruiguang, ZHANG Gaiping. Isolation,Identification and Genetic Characterization of Goose Astrovirus Isolate XX[J]. Journal of Henan Agricultural Sciences, 2021, 50(6): 134-141.

金前跃, 郭永刚, 李俊朋, 秦保亮, 王寅彪, 郭振华, 王丽, 邢广旭, 邓瑞广, 张改平. 鹅星状病毒XX株的分离鉴定及遗传特征分析[J]. 河南农业科学, 2021, 50(6): 134-141.

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Isolation,Identification and Genetic Characterization of Goose Astrovirus Isolate XX

鹅星状病毒XX株的分离鉴定及遗传特征分析

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