Isolation，Identification and Antibiotic Susceptibility Test of Aeromonas caviae from Common Carp（Cyprinus carpio）

doi:10.15933/j.cnki.1004-3268.2025.01.016

Abstract

Abstract: The research was conducted to clarify the etiology of common carp fingerlings death in a aquaculture farm in Wanzhou District，Chongqing，and provide references for the prevention and treatment of the disease.The pathogen was identified by separation and purification，physiological and biochemical tests，16S rDNA and cpn60 gene detection，phylogenetic analysis，and animal regression infection test.Antibiotic susceptibility test was carried out by disk diffusion method.The results showed that one dominant bacterium strain WZ2302 was isolated from the liver，kidney and spleen of the diseased fingerlings.The isolate was Gram‑negative，rod‑shaped and both ends were obtuse.The surface of the colony was smooth，with light yellow and semi transparent. The bacteria had motility.The oxidase，arginine dihydrolase，catalase，and indole tests were positive. Glucose（producing acid but no gas），sucrose，esculin，arabinose，mannitol，and salicin could be fermented. 16S rDNA and cpn60 gene sequences had the highest homology with Aeromonas caviae，reaching 99% and 98% respectively.The artificial infection symptoms performed similarity to the clinical case. The results of antibiotic susceptibility test showed that the bacteria were sensitive to 13 antibacterial drugs such as enrofloxacin，florfenicol，and ofloxacin；moderately sensitive to ciprofloxacin，streptomycin，and ciprofloxacin；resistant to penicillin，cefalotin，ampicillin and cefoperazone.In summary，the pathogen causing common carp fingerlings death was Aeromonas caviae，and enrofloxacin or florfenicol can be used to prevent and treat
the disease.

Key words: Cyprinus carpio, Aeromonas caviae, 16S rDNA, Isolation and identification, Antibiotic susceptibility test

摘要： 为明确重庆市万州区某养殖场鲤苗种死亡的病因，为该病的防治提供参考，通过细菌分离纯化、生理生化试验、16S rDNA和cpn60基因检测、系统进化分析、动物回归感染试验对病原进行鉴定，并采用纸片扩散法检测分离株的药敏特性。结果显示，从患病鲤苗种的肝脏、肾脏和脾脏中分离获得1株优势菌（WZ2302），革兰氏染色阴性，菌体两端钝圆，整体呈杆状；菌落表面光滑、颜色为浅黄色半透明；有运动性，氧化酶、精氨酸双水解酶、过氧化氢酶、吲哚试验呈阳性，能发酵葡萄糖（产酸不产气）、蔗糖、七叶苷、阿拉伯糖、甘露醇、水杨苷；16S rDNA和cpn60基因序列与豚鼠气单胞菌同源性最高，分别为99%和98%；回归试验攻毒组与临床病例表现相似。药敏试验结果显示，该菌对恩诺沙星、氟苯尼考、氧氟沙星等13种药物敏感；对环丙沙星、链霉素和麦迪霉素敏感性较差，对青霉素、头孢噻吩、氨苄西林和头孢哌酮存在耐药性。综上，引起重庆市万州区某养殖场鲤鱼苗种死亡的病原菌是豚鼠气单胞菌，可选用恩诺沙星或氟苯尼考进行防治。

关键词: 鲤, 豚鼠气单胞菌, 16S rDNA, 分离鉴定, 药敏试验

CLC Number:

S943

ZHANG Chao, LÜ Jun. Isolation，Identification and Antibiotic Susceptibility Test of Aeromonas caviae from Common Carp（Cyprinus carpio）[J]. Journal of Henan Agricultural Sciences, 2025, 54(1): 153-159.

张超, 吕军. 鲤源豚鼠气单胞菌的分离鉴定与药敏特性研究[J]. 河南农业科学, 2025, 54(1): 153-159.

Figures/Tables 7

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