Abstract: In order to develop long-lasting repellent for ticks, four repellent formulations with 20% IR3535 content were designed. According to the physical and chemical characteristics of IR3535,with IR3535 as active component, absolute ethanol as solvent, α-pyrrolidone as stabilizer, PEG-400 as tackifier,PVP-K30, CMC-Na and maltodextrin as slow-release components combined in a variety of solvent systems to design.The results of stability test showed that the solvent formulation with 5%—20%PVP,5%—20% α-pyrrolidone,5%—20% PEG-400 and 30% anhydrous ethanol had the best stability.The content of mosquito repellent was determined by gas chromatography.The results showed that the average intra day precision was 0. 59%,the average inter day precision were 0.49%,and the average recovery was 97.4%.The content of three batches of long-acting repellent was between 98.1% and 103.8% of the labeled amount.To sum up,the preparation of IR3535 long-acting tick repellent agent is simple,stable and repeatable.

Key words: IR3535, Tick, Content determination, Long-term repellent, Gas chromatography

摘要： 为研制长久持效的硬蜱驱避剂，依据驱蚊酯的理化特性，以驱蚊酯为有效成分、无水乙醇为溶剂、α-吡咯烷酮为稳定剂、聚乙二醇-400（PEG-400）为增黏剂，分别以聚乙烯吡咯烷酮（PVP-K30）、羧甲基纤维素钠(CMC-Na)、麦芽糊精为缓释成分进行多种溶剂系统组合，设计4个驱蚊酯含量为20%的驱避剂原药配方，并进行系列稳定性试验，对配方进行筛选，并对筛选出的配方进行相关含量测定。结果表明，采用5%～20%的聚乙烯吡咯烷酮、5%～20%的α-吡咯烷酮、5%～20%的聚乙二醇-400、30%的无水乙醇为组成成分的原药配方具有最好的稳定性。利用气相色谱法测定制剂中驱蚊酯的含量，所制备的驱蚊酯长效驱避剂的平均日内精密度为0.59%，平均日间精密度为0.49%，平均回收率为97.4%，3个批次的驱蚊酯长效驱避剂含量均介于标示量的98.1%～103.8%。综上，制备的驱蚊酯长效驱避剂工艺简单，稳定性好，可重复性高。

关键词: 驱蚊酯, 硬蜱, 含量测定, 长效驱避剂, 气相色谱法