doi:10.3969/j.issn.1004-3268.2007.10.029

Journal of Henan Agricultural Sciences ›› 2007, Vol. 36 ›› Issue (10): 93-94.DOI: 10.3969/j.issn.1004-3268.2007.10.029

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Published:2007-10-15 Online:2007-10-15

一种牡丹花瓣总RNA的提取方法

高双成;施江;王世华;孔祥生;张苗霞

河南科技大学农学院,河南洛阳471003

基金资助:
河南省科技发展计划项目（42301200）；河南科技大学博士科研启动经费（09001163）资助

Abstract

摘要： 利用CTAB改良法,以富含花青素类物质的牡丹花瓣为材料提取总RNA,经紫外光谱分析A260/A280比值为1.9-2.0;电泳检测到28S,18S和5S rRNA3条清晰的带,带的亮度强,且28S是18S亮度的2倍左右,说明提取的RNA完整、未降解。

关键词: 牡丹, 花瓣, 总RNA, CTAB法

CLC Number:

S685.11

高双成;施江;王世华;孔祥生;张苗霞. 一种牡丹花瓣总RNA的提取方法[J]. 河南农业科学, 2007, 36(10): 93-94.

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一种牡丹花瓣总RNA的提取方法

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