Effect of Low Temperature Treatment on Nuclear Morphology and Physiological Indexes of Peony Petals

doi:10.15933/j.cnki.1004-3268.2022.01.014

Abstract

Abstract: To analyze the cytological mechanism and physiological reasons of peony cut flower senescence at different temperatures，the effect of（25±1）℃（room temperature，contrast），2℃，4℃ and 8℃ on the vase life of peony was studied.The changes of nucleus morphology were observed and analyzed by DAPI，and the variations of protective enzyme（SOD，POD，CAT）activities，malondialdehyde（MDA），soluble protein content and superoxide anion（O-·2）generation rate during the process of flower arrangement in vase were analyzed.The results showed that the longest cut flower life was 17 d at 4，2℃.The order of the effect of different temperature treatments on the vase life of cut flowers was 4℃ =2℃ >8℃ .Low temperature treatment affected the normal rate of nucleus of cut flowers to different degrees. The normal rate of nucleus was basically 4℃>2℃>8℃，and the normal rate of nuclear could be used as an index to measure the health of cut flowers.The activities of SOD，CAT and POD of petals maintained a relatively high level，the petals could effectively remove excess reactive oxygen species and free radicals，and the metabolism was basically balanced，which was the physiological reason for the longer lifespan of cut flowers at 4℃.

Key words: Peony, Cut flower, Low temperature storage, Enzymatic activity, Nuclear

摘要： 为解析不同温度下牡丹凤丹粉切花衰老的细胞学机制及生理原因，以凤丹粉切花为材料，研究（25±1）℃（室温对照）、2℃、4℃和8℃处理对牡丹凤丹粉切花瓶插寿命的影响；采用DAPI染色观察分析花瓣细胞核形态变化规律并分析瓶插过程中保护酶［超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）］活性、丙二醛（MDA）含量、可溶性蛋白含量以及超氧阴离子（O-·2）产生速率的变化。结果表明，与对照［室温（25±1）℃］相比，4、2℃处理切花瓶插寿命最长，达17 d，低温处理下切花瓶插寿命依次为4℃=2℃>8℃。低温处理不同程度影响切花细胞核正常率，细胞核正常率基本为4℃>2℃>8℃，细胞核正常率可作为衡量切花健康程度的指标。花瓣SOD、CAT、POD活性保持较高水平，花瓣能有效清除过多的活性氧、自由基，代谢基本保持平衡，是4 ℃下切花寿命较长的生理原因。

关键词: 牡丹, 切花, 低温贮藏, 酶活性, 细胞核

CLC Number:

S685.11

CHEN Yue, LIU Huichao, JIA Wenqing, HE Songlin, GUO Yingzi, ZHU Xiaopei, WANG Yanli, DU Xiaohua, WANG Zheng. Effect of Low Temperature Treatment on Nuclear Morphology and Physiological Indexes of Peony Petals[J]. Journal of Henan Agricultural Sciences, 2022, 51(1): 116-124.

陈悦, 刘会超, 贾文庆, 何松林, 郭英姿, 朱小佩, 王艳丽, 杜晓华, 王政. 低温处理对牡丹花瓣细胞核形态及生理指标的影响[J]. 河南农业科学, 2022, 51(1): 116-124.

Figures/Tables 6

References

［1］VAN DOORN W G，BEERS E P，DANGL J L，et al.Morphological classification of plant cell deaths［J］.Cell Death and Differentiation，2011，18（8）：1241‐1246.
［2］FERNANDA V，MENGUER P，CAROLINE C‐C，et al.Programmed cell death（PCD） control in plants：New insights from the Arabidopsis thaliana deathosome［J］.Plant Science，2020，299：110603.
［3］曹慧，李春霞，王孝威.水分胁迫诱导八棱海棠和平邑甜茶细胞程序性死亡的研究［J］.园艺学报，2009，36（4）：469‐474.
CAO H，LI C X，WANG X W.Research of programmed cell ceath under water stress in Malus robusta Rehd.and Malus hupehensis（Pamp.）Rehd［J］.Acta Horticulturae Sinica，2009，36（4）：469‐474.
［4］李超.小麦花后干旱胁迫下乙烯和脱落酸对胚乳PCD调控机理研究［D］.石河子：石河子大学，2018.
LI C.The regulation of abscisic acid on PCD in wheat（Triticum aestivum L.）endosperm under drought stress post anthesis［D］.Shihezi ：Shihezi University，2018.
［5］潘海春.月季花发育过程中花瓣细胞程序化死亡机制研究［D］.泰安：山东农业大学，2004.
PAN H C.programmed cell death in petal during rose（Fosa×Hybrida） flower development ［D］.Taian：Shandong Agricultural University，2004.
［6］罗石磊.硫化氢对镉胁迫下黄瓜幼苗根尖细胞程序性死亡的影响［D］.兰州：甘肃农业大学，2020.
LUO S L.Effect of hydrogen sulfide on programmed cell death of cucumber（Cucumis sativus L.） rot tip under cadmium stress ［D］.Lanzhou： Gansu Agricultural University，2020.
［7］SERAFINI F D，DEL D S，MONTI F，et al.Transglutaminase activity during senescence and programmed cell death in the corolla of tobacco（Nicotiana tabacum） flowers［J］.Cell Death and Differentiation，2002，9（3）：309‐321.
［8］魏鑫，魏永祥，郭丹，等.低温处理后4个越橘品种部分生理指标的比较及其抗寒性分析［J］.植物资源与环境学报，2016，25（2）：72‐79.
WEI X，WEI Y X，GUO D，et al.Comparison on some physiological indexes of four cultivars of Vaccinium spp.after treated by low temperature and their cold resistance analysis ［J］.Journal of Plant Resources and the Environment，2016，25（2）：72‐79.
［9］黄晓霞，魏东玲，程小毛.低温处理对云南含笑幼苗生理特性的影响［J］.西南农业学报，2013，26（3）：973‐977.
HUANG X X，WEI D L，CHENG X M.Effect of low temperature on physiological traits of Michelia yunnanensis seedlings［J］.Southwest China Journal of Agricultural Sciences，2013，26（3）：973‐977.
［10］李咏富，哈益明，李伟明，等.不同处理方式对甜樱桃冰温贮藏效果影响的研究［J］.核农学报，2013，27（11）：1675‐1680.
LI Y F，HA Y M，LI W M，et al.Effects of different treatments on controlled biological freezing point storage of sweet cherry［J］.Nuclear Agriculture Journal，2013，27（11）：1675‐1680.
［11］于晓萌.玉蝉花切花保鲜及水晶干燥花制作技术研究［D］.哈尔滨：东北林业大学，2017.
YU X M.Study on enhancing vase‐life of Iris cut flower and techniques on making crystal flower of Iris ensata［D］.Harbin：Northeast Forestry University，2017.
［12］徐瑾，石印，刘芊，等.超低温保存后玉兰花粉的氧化应激和细胞凋亡研究［J］.植物生理学报，2015，51（6）：916‐920.
XU J，SHI Y，LIU Q，et al.Oxidative stress and or apoptosis of magnolia denudata pollen after cryopres‐ervation［J］.Plant Physiology Communications，2015，51（6）：916‐920.
［13］贾文庆，郭英姿，王艳丽，等.贮存条件对卵叶牡丹花粉寿命的影响［J］.农业工程学报，2020，36（14）：307‐315.
JIA W Q，GUO Y Z，WANG Y L，et al.Effects of storage conditions on pollen longevity of Paeonia qiui［J］.Transactions of the Chinese Society of Agricultural Engineering，2020，36（14）：307‐315.
［14］龙琴，谢宇，许兰珍，等.溃疡病菌侵染早期柑橘细胞程序性死亡的响应特征及机制［J］.园艺学报，2020，47（6）：1047‐1058.
LONG Q，XIE Y，XU L Z，et al.Characteristics and mechanism of programmed cell death in response to citrus canker pathogen in the early stage of infection［J］.Acta Horticulturae Sinica，2020，47（6）：1047‐1058.
［15］史国安，郭香凤，张国海，等.不同发育时期牡丹切花瓶插生理特性的研究［J］.园艺学报，2010，37（3）：449‐456.
SHI G A，GUO X F，ZHANG G H，et al.Studies of vasing physicological characteristics of cut peony flowers in various development stages ［J］.Acta Horticulturae Sinica，2010，37（3）：449‐456.
［16］王荣花. 牡丹、芍药切花贮藏保鲜及其生理基础的研究［D］.咸阳：西北农林科技大学，2005.
WANG R H.Study on storage and postharvest physiology of cut flowers in Paeonia suffruticosa Andr. and Paeonia
lactiflora Pal［l D］.Xianyang：Northwest A&F University，2005.
［17］孟海燕. 牡丹切花规模化贮藏技术基础研究［D］.洛阳：河南科技大学，2012.
MENG H Y.Basic research of the tree peony cut flowers under large‐scale storage［D］.Luoyang：University of Science and Technology of Henan，2012.
［18］曹满，施江，史国安.低温锻炼对冰温贮藏牡丹切花抗冷性的影响［J］.西北植物学报，2017，37（10）：1996‐2002.
CAO M，SHI J，SHI G A.Effects of low‐temperature acclimation on cold resistance of tree peony cut flowers in ice temperature storage ［J］.Acta Botanica Boreali‐Occidentalia Sinica，2017，37（10）：1996‐2002.
［19］张玉，李伟.Ca（NO3）2对芍药切花瓶插期生理和观赏品质的影响［J］.北方园艺，2019（19）：81‐87.
ZHANG Y，LI W. Effects of Ca（NO3）2 treatments on preservation and ornamental value of Paeonia lactiflora
cut flowers during vase phase ［J］. Northern Horticulture，2019（19）：81‐87.
［20］尹燕枰，董学会.种子学实验技术［M］. 北京：中国农业出版社，2008：60‐63.
YIN Y P，DONG X H.Seed ology experimental technology［M］.Beijing：China Agricultural Press House，2008：60‐63.
［21］李合生.植物生理生化实验原理和技术［M］.北京：高等教育出版社，2000：164‐165.
LI H S.Experimental principles and techniques of plant physiology and biochemistry［M］.Beijing：Higher Education Press（HEP），2000：164‐165.
［22］蒋大程，高珊，高海伦，等.考马斯亮蓝法测定蛋白质含量中的细节问题［J］.实验科学与技术，2018，16（3）：119‐122.
JIANG D C，GAO S，GAO H L，et al.The details of protein content determination by Coomassie brilliant blue staining［J］.Experiment Science and Technology，2018，16（3）：119‐122.
［23］徐川梅，黄坚钦，王正加，等.山核桃花粉母细胞减数分裂及核型［J］.林业科学，2017，53（11）：77‐84.
XU C M，HUANG J Q，WANG Z J，et al.Meiosis of pollen mother cell and karyotype of Carya cathayensis［J］.Scientia Silvae Sinicae，2017，53（11）：77‐84.
［24］WANG Y J，JI S J，DAI H Y，et al.Changes in membrane lipid metabolism accompany pitting in blueberry during refrigeration and subsequent storage at room temperature［J］.Frontiers in Plant Science，2019，10（27）：829.
［25］WANG F，YANG Q Z，ZHAO Q F，et al.Roles of antioxidant capacity and energy metabolism in the maturity‐dependent chilling tolerance of postharvest kiwifruit［J］.Postharvest Biology and Technology，2020，168（3）：1‐6.
［26］DACOSTA L C，ARAÚJO F F，DE SOUSA SANTOS M N，et al.Vase life and rehydration capacity of dry‐stored gladiolus flowers at low temperature［J］.Ciência Rural，2017，47（2）：1‐6.
［27］葛蓓蕾，张萍，雅蓉，等.低温胁迫后增温对‘罗宾娜’百合生理特性的影响［J］.西南农业学报，2020，33（2）：284‐289.
GE B L，ZHANG P，YA R，et al.Effect of temperature increase after low‐temperature stress on physiological characteristics of ‘robina’ lily［J］.Southwest Agricultural Journal，2020，33（2）：284‐289.
［28］ICHIMURA K，YOSHIOKA S，YAMADA T.Exogenous mannitol treatment stimulates bud development and extends vase life of cut snapdragon flowers ［J］.Postharvest Biology and Technology，2016，113：20‐28.
［29］ICHIMURA K，YAMADA T，SHIMIZU‐YUMOTO H.Recent breakthroughs in postharvest physiology research and cut flower handling in Japan［J］.Horticulture Environment and Biotechnology，2009，50（6）：539‐545.
［30］YAMADA T，ICHIMURA K，VAN DOORN W G.DNA degradation and nuclear degeneration during programmed cell death in petals of Antirrhinum［J］.Argyranthemum and Petunia，2006，57（14）：10.
［31］LI C，LI C，WANG B B，et al.Programmed cell death in wheat（Triticum aestivum L.） endosperm cells is affected by drought stress［J］.Protoplasma，2018，255（4）：1039‐1052.
［32］KAMDEE C，KIRASAK K，KETSA S，et al.Vesicles between plasma membrane and cell wall prior to visible senescence of Iris and Dendrobium flowers［J］.Journal of Plant Physiology，2015，188（1）：37‐43.

[1]	LIU Yajun, WANG Wenjing, LI Min, HU Qiguo, LIU Guangqing, SUN Xiyun, CHU Fengli. Effects of Different Rotation Modes of Sweet Potato on Soil Organic Carbon Components and Carbon Conversion Enzyme Activities [J]. Journal of Henan Agricultural Sciences, 2024, 53(2): 65-74.
[2]	ZHANG Sipu, ZHOU Qianqian, CUI Wei, ZHANG Ke, HAN Lixin, QU Zhenfang, LU Yunfeng, TANG Cunduo, NIU Jiajia. Effects of L‐tert‐leucine on Quality and Storage of Gala Apple [J]. Journal of Henan Agricultural Sciences, 2023, 52(12): 172-180.
[3]	WANG Huijuan, WANG Erqiang, FU Zhenzhu, LI Yanmin, WANG Xiaohui, WANG Zhanying, YUAN Xin, GAO Jie, WANG Limin, ZHANG Hechen. Phenotypic Diversity Analysis of Different Cultivars Groups of Herbaceous Peony Germplasm Resources [J]. Journal of Henan Agricultural Sciences, 2022, 51(12): 110-121.
[4]	TANG Fenfen, YANG Weike, XIE Kun, YUAN Shengyong, SHEN Dengrong, HE Chao, ZHANG Rui, LIU Na. Effect of BmNPV on the Activity of Trehalase and Its Gene Expression of Bombyx mori [J]. Journal of Henan Agricultural Sciences, 2021, 50(9): 157-162.
[5]	YANG Weike, TANG Fenfen, YANG Hai. Effect of Feeding Phoxim on the Alkaline Phosphatase Activity in Different Tissues of Bombyx mori [J]. Journal of Henan Agricultural Sciences, 2021, 50(4): 169-173.
[6]	LIU Xiaoxue, WANG Chunxia, LIU Lijuan, GUO Jinying, ZHENG Suyue. Identification and Mating Type Analysis of a Wild Oyster Mushroom Strain [J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 128-133.
[7]	TANG Fenfen, YANG Weike. Effects of Insect Hormone on ALP Activity and Its Gene Expression Level in Bombyx mori [J]. Journal of Henan Agricultural Sciences, 2020, 49(11): 153-158.
[8]	DING Xining, SHI Tian, YANG Linfei, YANG Hui, WANG Kaixuan, GUO Xiangfeng, SHI Guoan. Correlation between Seed Quality and Meteorological Factors of Oil Tree Peony Fengdan at Different Altitudes [J]. Journal of Henan Agricultural Sciences, 2019, 48(11): 120-126.