Cloning and Expression Analysis of EIN3/EIL Family Genes in Paeonia ostii cv‘.Phoenix White’

doi:10.15933/j.cnki.1004-3268.2022.03.013

Abstract

Abstract: Ethylene regulates plant nonbiological stress response and tolerance，and the EIN3/EIL family genes are key genes in the ethylene pathway. In this study we synthesized primers of three EIL genes referring to Paeonia suffruticosa and obtained three EIN3/EIL genes of Paeonia ostii cv.‘Phoenix White’，respectively named PoEIL1，PoEIL2，PoEIL3.Sequence analysis showed that they had high similarity to the Arabidopsis EIN3/EIL family genes，PoEIL1 clustered together with Arabidopsis AtEIL3 and PoEIL2 and PoEIL3 together with AtEIN3 and AtEIL1.Conservative motif analysis showed that each sequence had at least five conservative motifs at the same location.qRT⁃PCR analysis indicated that the three genes were expressed in each tissue，but the transcriptional levels varied.The expression level of the three genes in the petals of different flowering periods was also inconsistent.The expression level of PoEIL1 showed up⁃regulation at the opening stage，so it is speculated that PoEIL1 is related to flower development and flowering phase can be regulated by regulating PoEIL1 expression.

Key words: Paeonia suffruticosa, Paeonia ostii cv.‘Phoenix White’, EIN3/EIL, Gene cloning, Expression analysis

摘要： 乙烯调节植物非生物应激反应和耐受性，而EIN3/EIL家族基因是乙烯通路中的关键基因。参考牡丹3个EIL基因设计引物，获得3个凤丹白牡丹的EIN3/EIL基因，分别命名为PoEIL1、PoEIL2、PoEIL3（GenBank登录号为MZ648327—MZ648329）。序列分析表明，它们与拟南芥EIN3/EIL家族基因具有较高相似度，其中PoEIL1 与拟南芥的AtEIL3聚为一支，PoEIL2和PoEIL3与AtEIN3和AtEIL1 聚在一起。保守基序（Motif）分析结果显示，每条序列至少存在5个相同位置的保守基序。实时荧光定量PCR（qRTPCR）结果表明，3个基因在各组织中均有表达，但表达量各异。3个基因在不同花期花瓣中的表达特性也不一致，PoEIL1随着花的盛开表达量增加，推测PoEIL1与花的发育有关，可以通过调控PoEIL1 的表达来调控花期。

CLC Number:

S685.11

HAN Bo, XU Lingxia, CAO Xiaoying, ZHANG Guangqin. Cloning and Expression Analysis of EIN3/EIL Family Genes in Paeonia ostii cv‘.Phoenix White’[J]. Journal of Henan Agricultural Sciences, 2022, 51(3): 112-117.

韩波, 徐玲霞, 曹小迎, 张光琴. 凤丹白牡丹EIN3/EIL 家族基因的克隆与表达分析[J]. 河南农业科学, 2022, 51(3): 112-117.

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