Effect of Different Concentrations of Exogenous ZnONPs on the Heat Tolerance of Snapdragon

doi:10.15933/j.cnki.1004-3268.2024.12.014

Abstract

Abstract: Using snapdragon seedlings as test materials，this study investigated the effects of foliar spraying of different concentrations（0，50，100，200 mg/L）of zinc oxide nanoparticles（ZnONPs）on the growth，chlorophyll content，osmotic regulation substances，and enzyme activity and related substances in the AsA‑GSH cycle of snapdragon seedlings under day/night temperatures of 25℃/20℃，35℃/30℃ ，and 40℃/35℃ stress，in order to explore the role of ZnONPs in alleviating heat stress in snapdragon seedlings. The results showed that under high temperature stress，the height，stem thickness，leaf length，leaf width，and biomass of snapdragon significantly decreased.Applying different concentrations of ZnONPs could alleviate the growth inhibition of snapdragon. Under 40℃/35℃ stress，compared with the treatment of spraying 0 mg/L ZnONPs，the treatment of spraying 50—200 mg/L ZnONPs increased the total chlorophyll content and carotenoid content of snapdragon by 3.8%—7.7% and 4.9%—11.7%，respectively.The soluble sugar content and proline content of osmotic regulating substances increased by 12.7%—44.8% and 18. 2%—32.9%，respectively.Under 40 ℃/35 ℃ stress，the activities of ascorbate peroxidase（APX）， dehydroascorbate reductase （DHAR），monodehydroascorbate reductase（MDHAR），and glutathione reductase（GR）in the AsA‑GSH cycle of snapdragon continuously decreased. After spraying different concentrations of ZnONPs，the activities of APX，DHAR，MDHAR，and GR in snapdragon increased by 35.3%—86.3%，13.5%—24.6%，58.5%—81.5%，and 8.6%—19.7%，respectively. The content of ascorbic acid（AsA），glutathion（GSH），ascorbic acid/dehydroascorbate（AsA/DHA），and glutathione/reduced glutathione（GSH/GSSG） increased by 5.9%—11.7%，9.9%—16.5%，14.4%—31.1%，and 19.5%—37.7%，respectively，while the malondialdehyde（MDA）content，relative conductivity，O₂·^- production rate，and hydrogen peroxide（H₂O₂）content of snapdragon seedlings continued to decrease.The conclusion is that different treatments with ZnONPs can improve the heat tolerance of snapdragon，among which spraying 100 mg/L ZnONPs treatment has the best effect on improving the heat tolerance of snapdragon seedlings.

Key words: Snapdragon, Heat stress, ZnONPs, AsA?GSH, Chlorophyll, Active oxygen

摘要： 以金鱼草幼苗为试材，探究昼/夜温度25 ℃/20 ℃、35 ℃/30 ℃、40 ℃/35 ℃胁迫下叶面喷施不同质量浓度（0、50、100、200 mg/L）氧化锌纳米颗粒（ZnONPs）对金鱼草幼苗生长、叶绿素含量、渗透调节物质及AsA-GSH循环中酶活性及相关物质的影响，以探明ZnONPs缓解金鱼草热胁迫的作用。结果表明，高温胁迫下金鱼草株高、茎粗、叶长、叶宽以及生物量显著下降，施用不同质量浓度ZnONPs可以缓解金鱼草受到的生长抑制。40 ℃/35 ℃胁迫下，与喷施0 mg/L ZnONPs处理相比，喷施50~200 mg/L ZnONPs处理的金鱼草总叶绿素含量和类胡萝卜素含量分别增加3.8%~7.7%和4.9%~11.7%，渗透调节物质可溶性糖含量以及脯氨酸含量分别增加12.7%~44.8%和18.2%~32.9%。40 ℃/35 ℃胁迫下，金鱼草AsA-GSH循环过程中抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）、单脱氢抗坏血酸还原酶（MDHAR）及谷胱甘肽还原酶（GR）活性不断降低，喷施不同浓度ZnONPs 后金鱼草APX、DHAR、MDHAR 以及GR 活性分别增加35.3%~86.3%、13.5%~24.6%、58.5%~81.5% 和8.6%~19.7%，抗坏血酸（AsA）含量、谷胱甘肽（GSH）含量、抗坏血酸/脱氢抗坏血酸（AsA/DHA）及谷胱甘肽/还原型谷胱甘肽（GSH/GSSG）提高5.9%~11.7%、9.9%~16.5%、14.4%~31.1%和19.5%~37.7%，金鱼草幼苗丙二醛（MDA）含量、相对电导率、超氧阴离子自由基（O_2·^-）产生速率、过氧化氢（H₂O₂）含量则不断下降。综上，不同ZnONPs处理均能够提高金鱼草耐热性，其中喷施100 mg/L ZnONPs处理提高金鱼草幼苗耐热性的效果最好。

关键词: 金鱼草, 高温胁迫, ZnONPs, AsA-GSH, 叶绿素, 活性氧

CLC Number:

S681.9

LIU Dan, MAO Fengling, BAI Jinxin. Effect of Different Concentrations of Exogenous ZnONPs on the Heat Tolerance of Snapdragon[J]. Journal of Henan Agricultural Sciences, 2024, 53(12): 139-148.

刘丹, 毛凤玲, 白金鑫. 不同质量浓度外源ZnONPs 对金鱼草耐热性的影响[J]. 河南农业科学, 2024, 53(12): 139-148.

References

［1］WANG J，WU H H，WANG Y C，et al.Small particles，big effects：How nanoparticles can enhance plant growth in favorable and harsh conditions［J］.Journal of Integrative Plant Biology，2024，66（7）：1274‑1294.
［2］王敬营，王佳旭，陈丽娜，等. 埃洛石纳米管对铜胁迫下小麦幼苗生长的影响［J］. 河南农业科学，2024，53（7）：28‑34.
WANG J Y，WANG J X，CHEN L N，et al. Effect of halloysite nanotubes on growth of wheat under copper stress［J］. Journal of Henan Agricultural Sciences，2024，53（7）：28‑34.
［3］韩铭，张全国，周楠，等.光催化纳米颗粒对光合细菌HAU-M1产氢的影响［J］.河南农业大学学报，2023，57（2）：316‑323.
HAN M，ZHANG Q G，ZHOU N，et al. Effect of photocatalytic nanoparticles on hydrogen production by photosynthetic bacteria HAU‑M1［J］. Journal of Henan Agricultural University，2023，57（2）：316‑323.
［4］和春昊，金钺，张晨昕，等. 携肠球菌Ace抗原的铁蛋白纳米粒制备与免疫效果检测［J］.河南农业大学学报，2020，54（4）：638‑642.
HE C H，JIN Y，ZHANG C X，et al. Preparation and immunoassay of ferritin nanoparticles carrying antigenic epitopes of Ace from Enterococcus feacalis［J］.Journal of Henan Agricultural University，2020，54（4）：638‑642.

［5］WANG P，LOMBI E，ZHAO F J，et al. Nanotechnology：A new opportunity in plant sciences［J］. Trends in Plant

Science，2016，21（8）：699‑712.

［6］安义伟，梁慧慧，仲崇佳，等. 纳米基因载体在植物遗传转化中的应用进展［J］.河南农业科学，2022，51（12）：1‑9.

AN Y W，LIANG H H，ZHONG C J，et al.Progress on application of nano‑gene vector in plant genetic transformation ［J］. Journal of Henan Agricultural Sciences，2022，51（12）：1‑9.

［7］DENG C H，TANG Q，YANG Z M，et al.Effects of iron oxide nanoparticles on phenotype and metabolite changes in hemp clones（Cannabis sativa L.）［J］.Frontiers of Environmental Science & Engineering，2022，16（10）：134.

［8］孙露莹，宋凤斌，李向楠，等. 纳米氧化锌对玉米种子萌发及根系碳代谢的影响［J］. 土壤与作物，2020，9（1）：40‑49.

SUN L Y，SONG F B，LI X N，et al. Effects of ZnO nanoparticles on seed germination and root carbon metabolism in maize（Zea mays L. ）［J］. Soils and Crops，2020，9（1）：40‑49.

［9］彭晴晴，杨静雅，钟民正，等. ZnO NPs对四种豆科种子发芽及幼苗生长的影响［J］. 农业环境科学学报，2021，40（6）：1174‑1182.

PENG Q Q，YANG J Y，ZHONG M Z，et al. Effects of ZnO nanoparticles on the germination and seedling growth of four legume seeds［J］.Journal of Agro‑Environment Science，2021，40（6）：1174‑1182.

［10］胡灵璇，王晓红，张胜前，等.叶面施加纳米氧化锌对木槿生长及生理特性的影响［J］.湖南生态科学学报，2023，10（3）：51‑58.

HU L X，WANG X H，ZHANG S Q，et al.Effects of foliar application of zinc oxide nanoparticles in Hibiscus syriacus［J］.Journal of Hunan Ecological Science，2023，10（3）：51‑58.

［11］牟鲯璃，陈开俊，李雨航，等. 氧化锌纳米颗粒对生菜养分吸收及光合作用的影响［J］.浙江大学学报（农业与生命科学版），2023，49（2）：229‑240.

MOU Q L，CHEN K J，LI Y H，et al. Effects of zinc oxide nanoparticles on nutrient uptake and photosynthesis of lettuce［J］.Journal of Zhejiang University（Agriculture and Life Sciences），2023，49 （2）：229‑240.

［12］GUO J H，LI S X，BRESTIC M，et al. Modulations in protein phosphorylation explain the physiological responses of barley（Hordeum vulgare）to nanoplastics and ZnO nanoparticles［J］. Journal of Hazardous Materials，2023，443：130196.

［13］曹冲，黄娟，王宁，等. 纳米氧化锌对湿地植物种子萌发的影响［J］.东南大学学报（自然科学版），2017，47（2）：416‑420.

CAO C，HUANG J，WANG N，et al.Impact of zinc oxide nanoparticles on seed germination of wetland plant［J］.Journal of Southeast University（Natural Science Edition），2017，47（2）：416‑420.

［14］ VERMA S K，DAS A K，PATEL M K，et al. Engineered nanomaterials for plant growth and development：A perspective analysis ［J］. Science of the Total Environment，2018，630：1413‑1435.

［15］KHAN A R，AZHAR W，FAN X M，et al. Efficacy of zinc‑based nanoparticles in alleviating the abiotic stress in plants： Current knowledge and future perspectives［J］. Environmental Science and Pollution Research，2023，30（51）：110047‑110068.

［16］QIU J H，CHEN Y，LIU Z Q，et al. The application of zinc oxide nanoparticles：An effective strategy to protect rice from rice blast and abiotic stresses［J］.Environmental Pollution，2023，331：121925.

［17］PUJOL B，ARCHAMBEAU J，BONTEMPS A，et al.Mountain landscape connectivity and subspecies appurtenance shape genetic differentiation in natural plant populations of the snapdragon（Antirrhinum majus L.）［J］.Botany Letters，2017，164（2）：111‑119.

［18］陈宇华，陈剑锋，钟声远，等. 20份金鱼草种质资源花色性状鉴定与分析［J］.福建农业科技，2022，53（7）：1‑7.

CHEN Y H，CHEN J F，ZHONG S Y，et al.Identification and analysis of flower color traits of 20 germplasm resources of Antirrhinum majus［J］.Fujian Agricultural Science and Technology，2022，53（7）：1‑7.

［19］宋倩娜，刘琛，高振蕊，等. 热激启动子18. 2在金鱼草中启动下游基因表达最适温度条件的筛选［J］. 生态学杂志，2014，33（9）：2436‑2441.

SONG Q N，LIU C，GAO Z R，et al. Optimal temperature for hsp18. 2 promoter in gene expression of anthocyanin biosynthesis of Antirrhinum majus［J］.Chinese Journal of Ecology，2014，33（9）：2436‑2441.

［20］吴华. AsA-GSH循环参与海滨木槿盐应答机制的研究［D］. 济南：山东师范大学，2015.WU H. Study on the mechanism of AsA‑GSH cycle participating in salt response of Hibiscus littoral［D］.Ji’nan：Shandong Normal University，2015.

［21］王学奎. 植物生理生化实验原理和技术［M］. 2版. 北京：高等教育出版社，2006.WANG X K. Principles and techniques of plant physiological and biochemical experiments［M］. 2nd edition. Beijing：Higher Education Press，2006.

［22］王会涛，袁刘正，柳家友，等. 花期高温对玉米的影响研究进展［J］.河南农业科学，2022，51（9）：1‑9.

WANG H T，YUAN L Z，LIU J Y，et al. Research progress on effect of high temperature on maize at

flowering stage［J］. Journal of Henan Agricultural Sciences，2022，51（9）：1‑9.

［23］田佳，李佳，孟清波，等. 不同苹果品种叶片耐热阈值及高温下生理生化响应［J］. 河南农业科学，2021，50 （1）：121‑128.

TIAN J，LI J，MENG Q B，et al. Heat tolerance threshold and physiological and biochemical responses of leaves of different apple varieties［J］. Journal of Henan Agricultural Sciences，2021，50（1）：121‑128.

［24］袁慧敏，王革伏，樊佳茹，等. 高温对番茄幼苗生长和花芽分化的影响［J］. 西北植物学报，2019，39（10）：1768‑1775.

YUAN H M，WANG G F，FAN J R，et al. Effect of high temperature stress on growth and floral bud differentiation of tomato seedlings［J］. Acta Botanica Boreali‑Occidentalia Sinica，2019，39（10）：1768‑1775.

［25］KAREEM H A，HASSAN M U，ZAIN M，et al.Nanosized zinc oxide（n‑ZnO）particles pretreatment to alfalfa seedlings alleviate heat‑induced Morpho‑physiological and ultrastructural damages［J］.Environmental Pollution，2022，303：119069.

［26］聂青. SiO2提高匍匐型剪股颖和水稻耐高温胁迫的机理研究［D］. 北京：中国农业大学，2002.

NIE Q. Study on the mechanism of SiO2 enhancing creeping sheath and rice’s tolerance to high temperature stress［D］. Beijing：China Agricultural University，2002.

［27］江建成，廖菊阳，曹受金，等. 高温胁迫对三种杜鹃生长及生理的影响［J］. 北方园艺，2023（18）：54‑62.

JIANG J C，LIAO J Y，CAO S J，et al. Effects of high temperature stress on the growth and physiology of

three Rhododendron species［J］. Northern Horticulture，2023（18）：54‑62.

［28］刘晨，许业洲，杜超群，等. SiO2纳米颗粒对杉木幼苗生长发育的影响［J］. 中南林业科技大学学报，2020，40（4）：34‑43.

LIU C，XU Y Z，DU C Q，et al. Effects of SiO2 nanoparticles on growth and development of Cunninghamia lanceolata（Lamb.） Hook［J］. Journal of Central South University of Forestry & Technology，2020，40（4）：34‑43.

［29］刘敏，房玉林. 高温胁迫对葡萄幼树生理指标和超显微结构的影响［J］. 中国农业科学，2020，53（7）：1444‑1458.

LIU M，FANG Y L. Effects of heat stress on physiological indexes and ultrastructure of grapevines［J］. Scientia Agricultura Sinica，2020，53（7）：1444‑1458.

［30］刘辉，骆慧枫，张琛，等. 甜樱桃对高温胁迫的生理响应［J］.生态学报，2023，43（2）：702‑708.

LIU H，LUO H F，ZHANG C，et al. Physiological responses to high temperature stress in Prunus avium L.［J］. Acta Ecologica Sinica，2023，43（2）：702‑708.

［31］李萍萍，曾明，李文海，等. 胡杨异形叶抗氧化能力的比较［J］. 北京林业大学学报，2019，41（8）：76‑83.

LI P P，ZENG M，LI W H，et al. Comparative study on antioxidant capacity of heteromorphic leaves of Populus euphratica［J］. Journal of Beijing Forestry University，2019，41（8）：76‑83.

［32］刘晨，吴楚. SiO2纳米颗粒对黄瓜“新唐山秋瓜”幼苗根系解剖结构和气体交换的影响［J］. 安徽农业大学学报，2020，47（1）：148‑154.

LIU C，WU C. Effects of SiO2 nanopaticles on root anatomy and gas exchange of Cucumis sativus cv. “Qiugua of New Tangshan”［J］. Journal of Anhui Agricultural University，2020，47（1）：148‑154.

［33］MA Y X，HUANG J，CAO C，et al. Effects of silver nanoparticles on resistance characteristics of the wetland plant Typha orientalis in a hydroponic system［J］. Journal of Southeast University（English Edition），2019，35（3）：381‑388.
［34］柯博洋，李文龙，张彩英. 大豆SWEET基因在荚粒发育过程中与逆境胁迫下的表达［J］.中国农业科技导报，2023，25（8）：33‑52.
KE B Y，LI W L，ZHANG C Y. Expressions of SWEET genes during pod and seed developments and under different stress conditions in soybean［J］.Journal of Agricultural Science and Technology，2023，25（8）：33‑52.
［35］艾克热木·阿布拉，董宗炜，艾沙江·艾力，等. 和田河下游植被分布及生理指标对水分条件的响应［J］. 林业与环境科学，2022，38（5）：77‑87.
AIKEREMU A，DONG Z W，AISHAJIANG A，et al.Responses of vegetation distribution and physiological indexes to water condition in the lower reaches of Hetian River［J］. Forestry and Environmental Science，2022，38（5）：77‑87.
［36］龚束芳，刘阳，速馨逸，等.纳米硅肥对远东芨芨草幼苗模拟抗旱的影响［J］.草业科学，2018，35（12）：2924‑2930.
GONG S F，LIU Y，SU X Y，et al. Influence of nano‑silicon fertilizer on osmotic stress in Achnatherum extremiorientale［J］. Pratacultural Science，2018，35（12）：2924‑2930.
［37］SHEKHAWAT K， ALMEIDA‑TRAPP M，GARCÍA‑RAMÍREZ G X，et al. Beat the heat：Plantand microbe‑mediated strategies for crop thermotolerance［J］. Trends in Plant Science，2022，27（8）：802‑813.
［38］刘晓青，赵晖，耿兴敏，等.高温胁迫下杜鹃叶片AsA-GSH循环的亚细胞定位分析［J］.江苏农业科学，2021，49（18）：128‑133.
LIU X Q，ZHAO H，GENG X M，et al. Study on sub‑cellular distribution of AsA‑GSH cycle in Rhododendron leaves under high temperature stress［J］.Jiangsu Agricultural Sciences，2021，49（18）：128‑133.
［39］韩一林，王鑫朝，许馨露，等.毛竹幼苗抗氧化酶和AsA-GSH循环对高温干旱及协同胁迫的响应［J］. 浙江农林大学学报，2018，35（2）：268‑276.
HAN Y L，WANG X Z，XU X L，et al. Responses of anti‑oxidant enzymes and the ascorbate‑glutathione cycle to heat，drought，and synergistic stress in Phyllostachys edulis seedlings［J］.Journal of Zhejiang A & F University，2018，35（2）：268‑276.
［40］刘书仁，郭世荣，程玉静，等.外源脯氨酸对高温胁迫下黄瓜幼苗叶片AsA-GSH循环和光合荧光特性的影响［J］. 西北植物学报，2010，30（2）：309‑316.
LIU S R，GUO S R，CHENG Y J，et al. Effects of exogenous proline on the ascorbat‑glutahione cycle and photosynthetic fluorescence characteristics in leaves of cucumber seedlings under high temperature stress［J］.Acta Botanica Boreali‑Occidentalia Sinica，2010，30（2）：309‑316.
［41］孙军利，赵宝龙，郁松林. SA对高温胁迫下葡萄幼苗AsA-GSH循环的影响［J］.核农学报，2015，29（4）：799‑804.
SUN J L，ZHAO B L，YU S L. Effects of exogenous salicylic acid（SA） on ascorbate glutathione cycle（AsA‑GSH）circulation metabolism in grape seedlings under high temperature stress［J］.Journal of Nuclear Agricultural Sciences，2015，29（4）：799‑804.
［42］ZHAO L J， BAI T H， WEI H， et al.Nanobiotechnology‑based strategies for enhanced crop stress resilience［J］.Nature Food，2022，3：829‑836.
［43］XU W L，FENG Y C，DING Z X，et al.Peroxidase like Zn doped Prussian blue facilitates salinity tolerance in winter wheat through seed dressing［J］. International Journal of Biological Macromolecules，2024，267（Pt2）：131477.
［44］杨素爽，黄雅茹，郭雨亭，等. SiO2NPs纳米材料对草莓生长和高温逆境的作用［J］. 中国南方果树，2023，52（3）：181‑185.
YANG S S，HUANG Y R，GUO Y T，et al. Effects of SiO₂NPs nano‑materials on strawberry growth and high
temperature stress［J］. South China Fruits，2023，52（3）：181‑185.

[1]	LI Yuanyuan, ZHANG Wenjing, LI Nan. Effects of PGPR on Arsenic Accumulation and Physiological Characteristics of Aquatic Plants under Arsenic Stress [J]. Journal of Henan Agricultural Sciences, 2024, 53(6): 67-78.
[2]	YANG Yan, XIAO Bin. Effects of Exogenous Melatonin on the Photosynthesis，ASA‑GSH Cycle，and Hormone Changes of Malus‘Royalty’under Drought Stress [J]. Journal of Henan Agricultural Sciences, 2024, 53(6): 100-110.
[3]	QI Xianke, LI Miao, LI Caihong, QU Chenling. Effects of PAW and Its Combined Action with LTP on Seed Germination and Seedling Growth of Wheat [J]. Journal of Henan Agricultural Sciences, 2024, 53(4): 20-29.
[4]	ZHOU Shifeng, QIN Renqiang. Regulation of Plant Growth⁃Promoting Bacteria and Exogenous 2，4⁃Epibrassinolide on Photosynthetic Physiology and Ion Transport of Rose Seedlings under Salt Stress [J]. Journal of Henan Agricultural Sciences, 2024, 53(10): 138-148.
[5]	MA Wei, WU Zhiming, YU Kesong. Study on Canopy Chlorophyll Estimation Model of Buckwheat with Different Selenium Levels Based on UAV Multispectrum [J]. Journal of Henan Agricultural Sciences, 2023, 52(3): 161-172.
[6]	WANG Yanqin, MENG Xiangang, LI Wuyang, LUO Guanghong. Effects of Chlamydomonas on Photosynthetic Physiological Indexes of Wheat Seedlings under Salt Stress [J]. Journal of Henan Agricultural Sciences, 2023, 52(10): 22-29.
[7]	ZHONG Luming, ZHAI Tingkai, HAO Siyi, LIN Biying, CHU Yufan, YANG Yuying, SHEN Baoying. Screening and Mechanism Exploration of Light Intensity during Grafting Healing of Cucumber [J]. Journal of Henan Agricultural Sciences, 2022, 51(7): 123-133.
[8]	TIAN Yangqing, MA Chunmei, ZHAO Qiang, WU Xueqin, LI Jiangyu. Effects of Phthalamic Acid on Dry Matter Accumulation，Nutrient Absorption and Yield of Cotton [J]. Journal of Henan Agricultural Sciences, 2022, 51(6): 67-75.
[9]	HAO He, ZHONG Cuihong, LI Xinze, WANG Haiying, WU Ya’nan, WANG Fengshen, LIU Guanhui, SHI Yuxiang, ZHANG Yongying. Effect of Fermented Traditional Chinese Medicine on Intestinal Flora Structure of Broilers under Heat Stress [J]. Journal of Henan Agricultural Sciences, 2021, 50(9): 135-142.
[10]	SUN Lanlan, LI Jing, XUE Fei, XU Hongle, WU Renhai, SU Wangcang. Effect of Volunteer Wheat at Different Densities on Photosynthetic Characteristics and Yield of Cultivated Wheat [J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 103-110.
[11]	SUN Haoyue, WU Hongbin, LI Ming, ZHANG Qi, HAN Yiqiang, DU Jidao. Effects of Melatonin Seed Soaking on Growth and Physiological Characteristics of Kidney Bean Seedlings under Salt Stress [J]. Journal of Henan Agricultural Sciences, 2021, 50(12): 111-120.
[12]	SHI Yingying, HE Dong, YAO Xiaoyan, LUO Le, ZHANG Qixiang. Comparison on Chlorophyll Fluorescence Characteristics of Four Species of Primulina under Drought Stress [J]. Journal of Henan Agricultural Sciences, 2020, 49(9): 129-135.
[13]	WANG Yanzhao, ZHOU Bo, HAN Xiaohua, HUANG Bao, LU Xiaomin, CHENG Junling, WANG Shufeng, NIE Lihong. Genome-Wide Identification of Maize GATA Gene Family and Expression Analysis under Heat Stress [J]. Journal of Henan Agricultural Sciences, 2020, 49(11): 19-25.
[14]	LIU Wenwen, JI Fang, WANG Huahua. The Ｒole of Hydrogen Sulphide in Improving Aluminium Tolerance of Soybean [J]. Journal of Henan Agricultural Sciences, 2019, 48(10): 64-69.
[15]	NIU Yangli, ZHU Xiaopei, YANG Yaping, GE Xiaomin, DU Xiaohua, LIU Huichao. Cloning and Expression Analysis of HSP70 Gene in Different Heat-Ｒesistant Pansy(Viola tricolor) Materials under Heat Stress [J]. Journal of Henan Agricultural Sciences, 2019, 48(10): 127-132.