Analysis of MYB Transcription Factors Related to Cold Stress Response in Phalaenopsis Based on RNA‑sequencing

doi:10.15933/j.cnki.1004-3268.2024.08.012

Abstract

Abstract: In order to explore the function of MYB transcription factor in the response of cold‑resistant Phalaenopsis to low temperature stress，this research obtained a total of 31 MYB transcription factors with complete ORF from transcriptome library of Phalaenopsis cultivars under low temperature stress，and analyzed their structural domain，physicochemical properties，phylogenetic evolution，and expression characteristics. The results were as follows：there were 19 sequences belonging to R2R3‑MYB，with W（T/‑S/I）X₂EDX₂LX₇GX₃WX₂（L/V/I）X₃（A/T/S）（G/S）LXR（C/T/S）GKSCRLRWXNY of the amino acid arrangement pattern for R2 repeat，and（F/I/M/L）（S/T）X₂EX₃（I/V）（I/L/V）X（L/V）HX₂（L/W）G（N/T）（R/K）‑W（S/A）XIAX₂LPGRTDNE（I/V）KNXW（N/R/H）（T/S/G）of the amino acid arrangement pattern for R3 repeat；there were 12 sequences belonging to 1R‑MYB，with W（S/T）X（E/D）EHX₂FLX（A/G）X₄（G/D）‑（R/K）G0‑1（A/D）W or W（S/T）X（E/K）（Q/E）（N/D）KXFE（R/K）AL（A/V）X₃（E/D）X（T/A）PXRW of the amino acid arrangement pattern for R repeat.All these 31 sequences had a Myb‑DNA‑binding domain.The average relative molecular weight and theoretical isoelectric point were 30 288.28 and 7.55 for 31 MYB proteins in Phalaenopsis，respectively；all of them were hydrophobic proteins.MYB transcription factor TRINITY_DN61754_c4_g1 was almost unchanged in Phalaenopsis cultivar Big Chili among the whole process，while the expression level was decreased in the early stage of cold treatment in Phalaenopsis cv.Fuller’s Sunset.The expression level of RINITY_DN74288_c0_g1 was increased obviously at 48 h after cold treatment in Big Chili，however，the level was decreased remarkablely at 24 h after cold stress compared to normal temperature in Fuller’s Sunset.Based on the phylogenetic analysis and functional prediction of MYB transcription factors between in Phalaenopsis equestris and Phalaenopsis cultivars，it is speculated that these two genes may play important roles in response to low temperature stress in Phalaenopsis.

Key words: Phalaenopsis, MYB transcription factors, Low temperature stress, Gene expression, Gene mining

摘要： 为了探究MYB转录因子在抗冷型蝴蝶兰低温胁迫响应中的功能，从蝴蝶兰低温胁迫转录组文库中筛选出31条具有完整开放阅读框的MYB转录因子基因序列，对其蛋白质结构域、理化性质、系统进化、表达特性等进行分析。结果表明，有19条序列属于R2R3-MYB，其中所含R2氨基酸排列模式为W（T/S/I）X₂EDX₂LX₇GX₃WX₂（L/V/I）X₃（A/T/S）（G/S）LXR（C/T/S）GKSCRLRWXNY，R3氨基酸排列模式为（F/I/M/L）（S/T）X₂EX₃（I/V）（I/L/V）X（L/V）HX₂（L/W）G（N/T）（R/K）W（S/A）XIAX₂LPGRTDNE（I/V）KNXW‑（N/R/H）（T/S/G）；其余12条序列属于1R-MYB，R氨基酸排列模式为W（S/T）X（E/D）EHX₂FLX（A/G）X₄‑（G/D）（R/K）G0~1（A/D）W或W（S/T）X（E/K）（Q/E）（N/D）KXFE（R/K）AL（A/V）X₃（E/D）X（T/A）PXRW。这31条序列均具有Myb-DNA-binding结构域，平均相对分子质量为30 288.28，平均理论等电点为7.55，且都为疏水蛋白。蝴蝶兰MYB转录因子TRINITY_DN61754_c4_g1在抗冷品种大辣椒中的表达量于低温处理前后基本不变，但在不抗冷品种富乐夕阳中低温处理早期表达量就开始下降；TRINITY_DN74288_c0_g1在大辣椒中低温处理后48 h表达量显著增加，而在富乐夕阳中低温处理24 h后的表达量显著降低。结合其与小兰屿蝴蝶兰MYB转录因子的同源性分析及功能预测，推测这2个基因可能在蝴蝶兰低温胁迫响应过程发挥重要的作用。

关键词: 蝴蝶兰, MYB转录因子, 低温胁迫, 基因表达, 基因挖掘

CLC Number:

S682.31

LIANG Fang, ZHANG Yan, YUAN Xiuyun, KANG Yingying, WU Zheng, XU Shenping. Analysis of MYB Transcription Factors Related to Cold Stress Response in Phalaenopsis Based on RNA‑sequencing[J]. Journal of Henan Agricultural Sciences, 2024, 53(8): 108-117.

梁芳, 张燕, 袁秀云, 康莹莹, 吴征, 许申平. 基于转录组测序的蝴蝶兰低温胁迫响应MYB 转录因子挖掘[J]. 河南农业科学, 2024, 53(8): 108-117.

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