Abstract: In order to explore the structure and function of the protein encoded by the solute carrier 13(SLC13) gene family of bovine,this study used bioinformatics softwares to analyze the biological property(including the physicochemical properties,hydrophobicity,secondary structure,tertiary structure,subcellular localization and transmembrane region of the protein), predict the protein interaction networks and construct phylogenetic tree of the SLC13 gene family-encoded proteins of different species.The results showed that the number of amino acids encoded by the SLC13 gene family of bovine was from 520 to 626,the highest content was leucine and the lowest was histidine.The molecular mass ranged from 57.80 ku to 68.96 ku.All proteins were hydrophobic protein and all proteins except for SLC13A2 were stable.The secondary structure of the SLC13 protein gene was mainly composed of α-helix,β-turn and random coil,and the number of exons was from 11 to 16.Except that the SLC13A3 protein gene did not have Motif 10and the SLC13A5 protein did not have Motif 8, all proteins had Motif 1—10 in the sequence.The subcellular localization of SLC13 protein was in the plasma membrane, endoplasmic reticulum and mitochondria,and a small amount of SLC13A3 protein was found in the Golgi apparatus.The SLC13 protein was the inner membrane protein of multiple transmembrane helix,and it played a role in different forms at the biological level and molecular function level. Phylogenetic analysis found that the SLC13A1 gene of 14 species was in the same larger branch as the SLC13A4 gene,and SLC13A2,SLC13A3 and SLC13A5 were in another large branch.The common bovine had the closest relationship with zebu,buffalo and yak.

Key words: Bovine SLC13 gene family, Citrate cycle intermediates, Bioinformatics analysis, Phylogenetic tree, Protein interaction

摘要： 为研究牛溶质载体13（SLC13）基因编码蛋白的结构和功能，运用生物信息学软件对牛SLC13蛋白的理化性质、疏水性、二级结构及三级结构、序列保守基序、亚细胞定位、跨膜区进行分析，预测其蛋白质互作网络并构建不同物种SLC13基因家族编码蛋白的系统发育树。结果表明，牛SLC13蛋白的氨基酸数介于520~626个，亮氨酸含量最高，组氨酸含量最低，分子质量介于57.80~68.96 ku，其编码蛋白均为疏水性蛋白，除SLC13A2蛋白外的4个编码蛋白均为稳定蛋白。牛SLC13蛋白的二级结构主要由α-螺旋、β-转角以及无规卷曲组成，外显子数目介于11~16，除SLC13A3基因编码蛋白无Motif 10、SLC13A5基因编码蛋白无Motif 8外，其他编码蛋白序列均存在Motif 1—10，SLC13蛋白亚细胞定位于质膜、内质网和线粒体，SLC13A3蛋白在高尔基体中有少量分布。牛SLC13蛋白均为多次跨膜螺旋的内在膜蛋白，其以不同形式在生物学层面和分子功能层面发挥功能。系统进化分析发现，14个物种的SLC13A1、SLC13A4蛋白处于同一个较大分支，SLC13A2、SLC13A3和SLC13A5处于另一个较大分支，普通牛均与瘤牛、水牛、牦牛亲缘关系最近。

关键词: 牛SLC13基因家族, 柠檬酸循环中间体, 生物信息学分析, 进化树, 蛋白质互作