Identification and Sequence Analysis of the MdLAC Gene Family Members in Apple

doi:10.15933/j.cnki.1004-3268.2021.07.015

Abstract

Abstract: In order to understand the function and phylogenetic relationship of the apple laccase（MdLAC）gene family，the members of the MdLAC gene family were identified by the bioinformatics method，at the same time，the physical and chemicalproperties，phylogenetic relationships，gene structure，conserved structure，amino acid sequence and chromosome location were analyzed. The results showed that 77 MdLAC gene family members were identified in apple. The length of the amino acid sequence of encoded proteins ranged from 323 to 611，the molecular weight ranged from 36.29 to 67.87 ku，and the isoelectric point（pI） ranged from 4.65 to 9.92. The results of subcellular localization prediction showed that most of the MdLAC proteins were located outside the cell，the phylogenetic tree analysis showed that the MdLAC gene family had 10 branches，and the conservative structure analysis showed that the MdLAC gene family was conservative in structure. Chromosome mapping revealed that 77 MdLAC genes were unevenly distributed on 18 chromosomes.

Key words: Apple, MdLAC gene family, Bioinformatics, Phylogenetic tree, Sequence alignment, Chromosome location

摘要： 为了解苹果漆酶（MdLAC）基因家族的功能和系统进化关系，利用生物信息学方法对苹果MdLAC 基因家族成员进行鉴定，同时分析其家族成员的理化性质、系统进化关系、基因结构、保守结构、氨基酸序列和染色体定位。结果显示，在苹果中共鉴定到77个MdLAC基因家族成员，编码蛋白质的氨基酸序列长度在323~611，分子质量在36.29~67.87 ku，等电点（pI）在4.65~9.92。亚细胞定位预测结果表明，大部分MdLAC蛋白定位在细胞外膜；系统进化树显示，苹果MdLAC基因家族成员聚为10支；保守结构分析表明，MdLAC基因家族结构较为保守；染色体定位发现，77个MdLAC基因不均匀地分布在18条染色体上。关键词：苹果；MdLAC基因家族；生物信息学；系统进化树；序列比对；染色体定位

关键词: 苹果, MdLAC基因家族, 生物信息学, 系统进化树, 序列比对, 染色体定位

CLC Number:

S661.1

YANG Yang, LIU Yuanyuan. Identification and Sequence Analysis of the MdLAC Gene Family Members in Apple[J]. Journal of Henan Agricultural Sciences, 2021, 50(7): 125-135.

杨阳, 刘媛媛. 苹果MdLAC 基因家族成员的鉴定与序列分析[J]. 河南农业科学, 2021, 50(7): 125-135.

Figures/Tables 7

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