Genome‑wide Identification and Expression Analysis of CCCH Type Zinc‑finger Gene Family in Nicotiana tabacum

doi:10.15933/j.cnki.1004-3268.2022.04.006

Journal of Henan Agricultural Sciences ›› 2022, Vol. 51 ›› Issue (4): 48-58.DOI: 10.15933/j.cnki.1004-3268.2022.04.006

• Crop Cultivation & Genetic Breeding • Previous Articles Next Articles

Genome‑wide Identification and Expression Analysis of CCCH Type Zinc‑finger Gene Family in Nicotiana tabacum

TANG Chungui¹，DENG Zhaolong²，LIU Qiong²，JIN Weihuan²，XIANG Shipeng¹，XIE Pengfei¹，GUO Hongxiang²，LIU Weiqun²

（1.Changsha Branch of Hunan Province Tobacco Company，Changsha 410011，China；2.College of Life Sciences，Henan Agricultural University，Zhengzhou 450002，China）

Received:2021-10-08 Published:2022-04-15 Online:2022-07-01

普通烟草CCCH类锌指蛋白家族的全基因组鉴定和表达分析

唐春闺¹，邓兆龙²，刘琼²，金维环²，向世鹏¹，谢鹏飞¹，郭红祥²，刘卫群²

（1.湖南省烟草公司长沙市公司，湖南长沙 410011；2.河南农业大学生命科学学院，河南郑州 450002）

通讯作者: 刘卫群（1956-），女，河北临城人，教授，博士，主要从事植物逆境分子生物学研究。E-mail：liuweiqun2004@126.com
作者简介:唐春闺（1979-），女，湖南邵阳人，农艺师，硕士，主要从事烟草栽培技术研究。E-mail：617335424@qq.com
基金资助:
基金项目：湖南省烟草公司长沙市公司科技项目（2019430100270173）

Abstract

Abstract: In order to understand the characteristics of tobacco CCCH type zinc‑finger gene family，bioinformatics methods were performed in the tobacco genome database using Arabidopsis CCCH typeprotein motif and 86 CCCH type zinc‑finger family members were identified. Protein physicochemical properties，systemic evolution，chromosome distribution，motif，promoter cis‑acting elements，gene expression pattern and the response to drought and phytohormone were analyzed. These results showed that 37 of 86 tobacco CCCH type zinc‑finger genes were distributed on 17 chromosomes，all members of tobacco CCCH type zinc‑finger proteins contained C‑X_7/8‑C‑X₅‑C₃‑H motif and some novel CCCH motifs were also found，such as C‑X₆‑C‑X₄‑C‑X₃‑H.Systemic evolution analysis indicated that 51 of 86 CCCH type zinc‑finger genes were divided into nine groups.Multiple cis‑regulatory elements including stress response elements and phytohormone response elements were found in the promoter region of NtC3H-39 gene. Results of gene expression patterns and stress response analysis revealed that NtC3H-39 gene showed obvious tissue specific expression and responses to drought stress and phytohormone stress.

Key words: Nicotiana tabacum, CCCH type zinc?finger genes, NtC3H-39, Stress response, Bioinformatics

摘要： 为了解烟草CCCH类锌指蛋白家族特性，利用拟南芥CCCH类锌指蛋白结构域比对烟草基因组数据库，共鉴定到86个CCCH类锌指蛋白基因家族成员，并对其进行蛋白质理化性质、系统进化、染色体分布、基序、启动子顺式作用元件、基因表达模式分析以及对激素的响应分析。结果显示，烟草CCCH类锌指蛋白家族86个基因中37个成员分布在17条不同的染色体上；基序分析显示，该家族所有成员均含有C-X_7/8-C-X₅-C₃-H基序，除此之外，在烟草中也发现了一些新的CCCH基序，如C-X₆-C-X₄-C-X₃-H 等；系统进化树显示，86 个CCCH 类锌指蛋白中的51 个成员分为9 个亚家族；启动子分析表明，NtC3H-39基因含有多个应激响应元件和激素响应元件。基因表达模式和激素响应分析表明，NtC3H-39具有明显的组织表达特异性，并对激素和干旱胁迫具有明显响应。

关键词: 烟草, CCCH类锌指蛋白基因家族, NtC3H-39, 胁迫应答, 生物信息学

CLC Number:

S572

TANG Chungui, DENG Zhaolong, LIU Qiong, JIN Weihuan, XIANG Shipeng, XIE Pengfei, GUO Hongxiang, LIU Weiqun. Genome‑wide Identification and Expression Analysis of CCCH Type Zinc‑finger Gene Family in Nicotiana tabacum[J]. Journal of Henan Agricultural Sciences, 2022, 51(4): 48-58.

唐春闺, 邓兆龙, 刘琼, 金维环, 向世鹏, 谢鹏飞, 郭红祥, 刘卫群. 普通烟草CCCH类锌指蛋白家族的全基因组鉴定和表达分析[J]. 河南农业科学, 2022, 51(4): 48-58.

Figures/Tables 10

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Genome‑wide Identification and Expression Analysis of CCCH Type Zinc‑finger Gene Family in Nicotiana tabacum

普通烟草CCCH类锌指蛋白家族的全基因组鉴定和表达分析

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