麦麸膳食纤维多酚复合物的理化性质和发酵特性研究

doi:10.15933/j.cnki.1004-3268.2025.10.018

摘要/Abstract

摘要： 为促进小麦加工副产物的高值化利用，对小麦麸皮（WB）和麦麸膳食纤维多酚复合物（SDF-P）的理化性质和发酵降解特性进行系统分析。首先测定其溶解度、膨胀度、持水/持油性、冻融稳定性和热特性等基本理化性质，继而测定流变特性；随后通过体外发酵试验，分析短链脂肪酸（SCFAs）含量、pH值动态变化及肠道菌群组成变化；最后对理化性质与发酵特性进行相关性分析。结果表明，SDF-P的溶解度显著高于WB，最高为87.51%，膨胀度、持水性、持油性、析水率显著低于WB，相变热焓、峰值温度及表观黏度均高于WB。随着体外发酵时间的延长，SCFAs含量增加，pH值下降，SDF-P发酵产生的SCFAs含量可达62.57 mmol/L；基因组测序显示，SDF-P男性发酵液中双歧杆菌属和乳酸杆菌属较空白组分别增加了72%和150%，大肠杆菌属降低了30%，表明SDF-P具有明显改善肠道菌群的作用。相关性分析表明，溶解度、相变热焓和峰值温度与SCFAs含量变化呈正相关关系，与pH值变化呈负相关关系。综上，SDF-P兼具高溶解度、优良热稳定性和显著促进有益菌增殖的功能特性，为小麦麸皮作为高品质膳食纤维-多酚配料应用于功能性食品开发提供了理论依据。

关键词: 小麦麸皮, 麦麸膳食纤维多酚复合物, 理化性质, 发酵降解特性

Abstract: To promote the high‐value utilization of wheat processing by‐products，the physicochemical properties and fermentation degradation characteristics of wheat bran（WB）and wheat bran dietary fiber polyphenol complex（SDF‐P） were systematically analyzed.Firstly，the basic physical and chemical properties such as solubility，bulk degree，water/oil holding capacity，freeze‐thaw stability and thermal properties were determined，and then the rheological properties were determined.Subsequently，the dynamic changes of the content of short‐chain fatty acids（SCFAs），pH value and the composition of intestinal flora were analyzed by in vitro fermentation experiments.Finally，the correlation between physicochemical properties and fermentation characteristics was analyzed.The results showed that the solubility of SDF‐P was significantly higher than that of WB，with the highest value of 87.51%.The swelling degree，water holding capacity，oil holding capacity and the water separation rate of SDF‐P were significantly lower than those of WB.The phase change thermal enthalpy，peak temperature and apparent viscosity of SDF‐P were higher than those of WB.With the prolongation of in vitro fermentation time，the SCFAs content increased and the pH value decreased.The SCFAs content produced by SDF‐P fermentation could reach 62.57 mmol/L.Genome sequencing showed that compared with blank group，Bifidobacterium and Lactobacillus in SDF‐P male fermentation broth increased by 72% and 150%，respectively，and Escherichia coli decreased by 30%.These results indicated that SDF‐P had a significant effect on improving intestinal flora.Correlation analysis indicated that solubility，phase change thermal
enthalpy，and peak temperature were positively correlated with changes in SCFAs content and negatively correlated with changes in pH.In summary，SDF‐P possesses high solubility，excellent thermal stability，and significant beneficial bacterial proliferation properties，providing a theoretical basis for the application of wheat bran as a high‐quality dietary fiber‐polyphenol ingredient in the development of functional foods.

Key words: Wheat bran, Wheat bran dietary fiber polyphenol complex, Physicochemical properties, Fermentation degradation characteristics

中图分类号:

张静雯, 刘芮杉, 申瑞玲, 李鹏冲. 麦麸膳食纤维多酚复合物的理化性质和发酵特性研究[J]. 河南农业科学, 2025, 54(10): 171-179.

ZHANG Jingwen, LIU Ruishan, SHEN Ruiling, LI Pengchong. Study on the Physicochemical Properties and Fermentation Degradation Characteristics of Dietary Fiber Polyphenol Complex of Wheat Bran[J]. Journal of Henan Agricultural Sciences, 2025, 54(10): 171-179.

图/表 8

参考文献

［1］AWIKA J M，ROSE D J，SIMSEK S. Complementary effects of cereal and pulse polyphenols and dietary fiber on chronic inflammation and gut health［J］.Food & Function，2018，9（3）：1389‐1409.

［2］ CROFT K D，FRAGA C G. Polyphenols and health［J］.Food & Function，2020，11（10）：8405‐8406.
［3］鲁朝凤，黄佳琦，黄勇桦，等. 青稞膳食纤维和多酚对肠道微生物的协同调节作用［J］.食品与发酵工业，2021，47（8）：6‐13.
LU C F，HUANG J Q，HUANG Y H，et al. Synergetic regulation of gut microbiota by dietary fiber and phenolic compounds in hulless barley［J］.Food and Fermentation Industries，2021，47（8）：6‐13.
［4］ZHANG L，WU T，ZHANG Y L，et al. Release of bound polyphenols from wheat bran soluble dietary fiber during simulated gastrointestinal digestion and colonic fermentation in vitro［J］.Food Chemistry，2023，402：134111.
［5］BERMÚDEZ‐ORIA A，RODRÍGUEZ‐GUTIÉRREZ G，FERNÁNDEZ‐PRIOR Á，et al. Strawberry dietary fiber functionalized with phenolic antioxidants from olives.Interactions between polysaccharides and phenolic compounds［J］.Food Chemistry，2019，280：310‐320.
［6］郑妍，隋勇. 膳食因子对多酚生物利用率影响的研究进展［J］.河南工业大学学报（自然科学版），2020，41（6）：111‐118.
ZHENG Y，SUI Y. Research progress on the effects of dietary factors on the bioavailability of polyphenol［J］.Journal of Henan University of Technology（Natural Science Edition），2020，41（6）：111‐118.
［7］HAN X B，MA Y，DING S J，et al. Regulation of dietary fiber on intestinal microorganisms and its effects on animal health［J］. Animal Nutrition，2023，14：356‐369.

［8］全文彬，王娟，黄继红，等. 小麦麸皮可溶性膳食纤维的理化性质和结构表征［J］. 食品与发酵工业，2024，50（21）：259‐265.
QUAN W B，WANG J，HUANG J H，et al.Physicochemical properties and structural characterization of soluble dietary fiber of wheat bran［J］.Food and Fermentation Industries，2024，50（21）：259‐265.
［9］许英一，张微，王宇，等. 超声对小麦麸皮蛋白结构和功能性质的影响［J］.中国粮油学报，2023，38（10）：53‐61.
XU Y Y，ZHANG W，WANG Y，et al. Effect of ultrasonic on structure and functional properties of wheat bran protein［J］.Journal of the Chinese Cereals and Oils Association，2023，38（10）：53‐61.
［10］李鹏冲.麦麸多酚膳食纤维复合物与全麦面粉的结构性质及发酵降解特性研究［D］.郑州：郑州轻工业学院，2018.
LI P C.Study on Structure，Properties and Fermentation Degradation Characteristics of Wheat Bran Polyphenol Dietary Fiber Complex and Whole Wheat Flour［D］. Zhengzhou：Zhengzhou University of Light Industry，2018.
［11］FU S J，LIU X R，DENG J W，et al. Effects of milling condition on nutritional，physicochemical，and textural properties of highland barley grains［J］.Cereal Chemistry，2023，100（4）：927‐935.
［12］YE G D，WU Y N，WANG L P，et al. Comparison of six modification methods on the chemical composition，functional properties and antioxidant capacity of wheat bran［J］. LWT，2021，149：111996.
［13］ZHANG L，ZENG L R，WANG X，et al. The influence of Konjac glucomannan on the functional and structural properties of wheat starch［J］.Food Science & Nutrition，2020，8（6）：2959‐2967.
［14]LIU D X，SONG S X，TAO L，et al. Effects of common buckwheat bran on wheat dough properties and noodle quality compared with common buckwheat hull［J］.LWT，2022，155：112971.
［15］LIU N，MA S，LI L，et al. Study on the effect of wheat bran dietary fiber on the rheological properties of dough［J］. Grain & Oil Science and Technology，2019，2（1）：1‐5.
［16］GONG L X，CHI H L，WANG J，et al. In vitro fermentabilities of whole wheat as compared with refined wheat in different cultivars［J］. Journal of Functional Foods，2019，52：505‐515.
［17］UERLINGS J，BINDELLE J，SCHROYEN M，et al.Fermentation capacities of fructan‐ and pectin‐rich by‐products and purified fractions via an in vitro piglet faecal model［J］. Journal of the Science of Food and Agriculture，2019，99（13）：5720‐5733.
［18］LI S Q，MENG Y T，WANG C，et al.Effect of structural characteristics of resistant starch prepared by various methods on microbial community and fermentative products ［J］. International Journal of Biological Macromolecules，2024，254：127725.
［19］CHENG W，SUN Y J，FAN M C，et al. Wheat bran，as the resource of dietary fiber：A review［J］. Critical Reviews in Food Science and Nutrition，2022，62（26）：7269‐7281.
［20］江帆，杜春微，任妍婧，等. 不同藜麦品种淀粉的理化性质与消化特性［J］.中国粮油学报，2021，36（7）：77‐83.
JIANG F，DU C W，REN Y J，et al. Physicochemical properties and digestibility of starches of different quinoa varieties［J］. Journal of the Chinese Cereals and Oils Association，2021，36（7）：77‐83.
［21］李欣，张洪微，刘莹，等. 小麦麸皮淀粉的提取及其结构分析［J］.粮油食品科技，2020，28（5）：111‐116.
LI X，ZHANG H W，LIU Y，et al. Extraction and structure analysis of wheat bran starch［J］. Science and Technology of Cereals，Oils and Foods，2020，28（5）：111‐116.
［22］DEVI R，SINGH A，DUTTA D，et al. Physicochemical properties of barley starch and effect of substitution of wheat flour with barley starch on texture and sensory properties of bread［J］. eFood，2024，5（1）：e132.
［23］FAN X，LI X P，HU J W，et al. Physicochemical and in vitro digestibility properties on complexes of fermented wheat starches with konjac gum［J］. International Journal of Biological Macromolecules，2021，188：197‐206.
［24］徐丽娜，张宁，杨国燕，等. 麦麸可溶性和不溶性膳食纤维对小麦淀粉性质的影响［J］.粮食与油脂，2024，37（1）：25‐29.

XU L N，ZHANG N，YANG G Y，et al. The effect of soluble and insoluble dietary fiber from wheat bran on the properties of wheat starch［J］. Cereals & Oils，2024，37（1）：25‐29.
［25］SAINI P，ISLAM M，DAS R，et al. Wheat bran as potential source of dietary fiber：Prospects and challenges［J］. Journal of Food Composition and Analysis，2023，116：105030.
［26］PARKAR S G，TROWER T M，STEVENSON D E.Fecal microbial metabolism of polyphenols and its effects on human gut microbiota［J］. Anaerobe，2013，23：12‐19.
［27］MARTEL J，CHANG S H，KO Y F，et al. Gut barrier disruption and chronic disease ［J］. Trends in Endocrinology and Metabolism，2022，33（4）：247‐265.
［28］CHEN B H，LIU G H，CHEN Q R，et al. Discovery of a novel marine Bacteroidetes with a rich repertoire of carbohydrate‐active enzymes［J］. Computational and Structural Biotechnology Journal，2024，23：406‐416.
［29］杨媚. 热处理对全谷燕麦β-葡聚糖和戊聚糖结构及体外发酵的影响［D］. 郑州：郑州轻工业学院，2018.
YANG M. Effects of thermal processing on the structural characteristics and in vitro fermentation of whole grain oats β‐glucan and arabinoxylan［D］.Zhengzhou：Zhengzhou University of Light Industry，2018.
［30］MARTINEZ T M，MEYER R K，DUCA F A. Therapeutic potential of various plant‐based fibers to improve energy homeostasis via the gut microbiota［J］. Nutrients，2021，13（10）：3470.
［31］LIU S，YU Q，HUANG H R，et al. The effect of bound polyphenols on the fermentation and antioxidant properties of carrot dietary fiber in vivo and in vitro［J］. Food & Function，2020，11（1）：748‐758.
［32］王兆丰，代华，高义霞，等. 多酚处理对甜樱桃采后品质和活性氧代谢的影响［J］. 南方农业学报，2024，55
（2）：578‐588.
WANG Z F，DAI H，GAO Y X，et al. Effects of polyphenol treatment on postharvest quality andreactive oxygen species metabolism in Prunus avium L.［J］. Journal of Southern Agriculture，2024，55（2）：578‐588.

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