关键词: 辛硫磷, 家蚕, 碱性磷酸酶, 组织, 酶活性

Abstract: In order to investigate the effect of phoxim on alkaline phosphatase(ALP) activity in different tissues of silkworm,the spectrophotometry was used to detect the changes of ALP activities in fat body,hemolymph,midgut and silk gland of Bombyx mori after feeding phoxim(in the control group sterile water was fed).The results showed that the midgut ALP activity was gradually increased at 6 h after feeding phoxim and reached a maximum value at 15 h,but it decreased sharply and was significantly lower than that of the control group at 24 h.The ALP activities in hemolymph and fat body were increased at 12 h and 15 h respectively after feeding phoxim and both of them reached higher level at 18 h.However,the ALP activity was inhibited at 21 h and 24 h and significantly lower than that of the control group.In silk gland,the ALP activity began to decline at 9 h and continued to decrease with the extension of treatment time.The enzyme activity decreased to 13% of the control group at 24 h and the difference between phoxim treatment group and control group reached an extremely significant level. The above results indicated that the ALP activity was inhibited to a certain extent in the silk gland after treatment with phoxim,while it showed a trend of increasing first and then decreasing sharply in the fat body,hemolymph and midgut of silkworm.It suggests that the ALP in fat body,hemolymph and midgut of silkworm may play an important role in the metabolizing of phoxim.

Key words: Phoxim, Bombyx mori, Alkaline phosphatase, Tissues, Enzymatic activity