关键词: 小麦, 条锈病, 成株期, Yr基因, 分子标记；抗病鉴定

Abstract: Two hundred and forty-two wheat germplasms from Guizhou,Sichuan and other places were collected,the adult-plant resistance to stripe rust was identified in the field,and five known resistance genes(Yr5,Yr10,Yr15,Yr18,Yr26) markers were used to detect the materials,so as to determine the stripe rust resistance level and type of disease-resistant genes of wheat germplasms,which would lay the foundation for the scientific and reasonable use of excellent disease-resistant wheat germplasms.The results showed that 116 wheat germplasms at adult stage displayed resistance to stripe rust among 242 wheat germplasms,including 11 germplasms were immune,27 germplasms were nearly immune,41 germplasms were highly resistant and 37 germplasms were moderately resistant to stripe rust,which accounted for 4.54%,11.16%,16.94% and 15.29% of the germplasms,respectively;only one germplasm exhibited slow rust,accounting for 0.41%;125 germplasms exhibited medium sensitivity and high sensitivity,accounting for 51.65%.Molecular detection result showed that,59 germplasms carried Yr26,13 contained Yr18,21 contained Yr15,14 contained Yr10,and 52 contained Yr5.There were 39 germplasms containing 2 resistant genes,such as Guinong No.19;7 germplasms carried 3 resistant genes,such as Guinong 775 and Guixie No.3;80F-1-4-2 contained 4 resistant genes.The above Yr genes were not detected in the remaining disease resistant germplasms,which might carry other undetected disease resistant genes.

Key words: Wheat, Stripe rust, Adult stage, Yr gene, Molecular marker, Disease resistance identification