河南农业科学 ›› 2025, Vol. 54 ›› Issue (3): 71-76.DOI: 10.15933/j.cnki.1004-3268.2025.03.008

• 作物栽培·遗传育种 • 上一篇    下一篇

印度梨形孢对濒危藏药薄叶鸡蛋参生长和生理特性的影响

柴国柱,王美汇,邱泽江,杨启帆,袁芳   

  1. (西藏农牧学院 西藏自治区藏药资源保护与利用重点实验室,西藏 林芝 860000)
  • 收稿日期:2025-01-09 出版日期:2025-03-15 发布日期:2025-04-23
  • 通讯作者: 袁芳(1983-),女,甘肃庆阳人,副教授,硕士,主要从事药用植物资源开发利用研究工作。E-mail:396531633@qq.com
  • 作者简介:柴国柱(2000-),男,河北秦皇岛人,在读硕士研究生,研究方向:农业生物技术。E-mail:2932143580@qq.com
  • 基金资助:
    西藏农牧学院研究生教育创新计划项目(YJS2024-01);林芝市科技计划项目(SYQ2024-02)

Effect of Piriformospora indica on Growth and Physiological Characteristics of Endangered Tibetan Medicine Codonopsis convolvulacea Kurz.var.vinciflora(Kom.)L.T.Shen

CHAI Guozhu,WANG Meihui,QIU Zejiang,YANG Qifan,YUAN Fang   

  1. (Key Laboratory of Tibetan Medicine Resources Conservation and Utilization of Tibet Autonomous Region,Tibet Agriculture and Animal Husbandry University,Linzhi 860000,China)
  • Received:2025-01-09 Published:2025-03-15 Online:2025-04-23

摘要: 为探究内生真菌印度梨形孢对栽培薄叶鸡蛋参的促生作用,提高其产量和抗逆性,采用室外盆栽试验,设置对照(CK)和接菌(PI)2个处理,研究印度梨形孢对薄叶鸡蛋参生长和生理特性的影响。结果表明,采用浸泡结合灌溉接种法,成功建立了印度梨形孢与薄叶鸡蛋参的共生体系,定殖率为70.37%。印度梨形孢定殖的薄叶鸡蛋参,与对照相比较,其叶片数、总茎数、主茎长度、地上部分鲜质量和干质量分别显著增加101.27%、102.41%、76.07%、278.23%、265.52%,须根数、须根长度、地下部分鲜质量和干质量分别显著增加52.57%、63.39%、23.65%、32.52%,块茎直径增加12.14%;过氧化氢酶(CAT)、过氧化物酶(POD)活性分别显著提高9.79%、19.35%,超氧化物歧化酶(SOD)活性降低了28.02%;可溶性糖、脯氨酸含量分别显著提高42.84%、92.19%;根系活力显著提高65.57%。综上所述,印度梨形孢能够促进薄叶鸡蛋参的生长和增强其抗逆性,可用于提高薄叶鸡蛋参的栽培质量。

关键词: 薄叶鸡蛋参, 印度梨形孢, 药用植物, 生长发育, 抗逆性

Abstract: In order to improve the yield and stress resistance of cultivated Codonopsis convolvulacea Kurz.var.vinciflora(Kom.)L.T.Shen,the promotion effects of endophytic fungus Piriformospora indica on its growth were explored.The effects of P.indica on the growth and physiological characteristics of C.convolvulacea were studied by outdoor pot experiment with two treatments of control group(CK)and inoculation group(PI).The results showed that the symbiotic system of P.indica and C.convolvulacea was successfully established by soaking and watering inoculation,and the colonization rate was 70.37%.Compared with CK,the leaf number,stem number,main stem length,aboveground fresh weight and dry weight of C.convolvulacea colonized by P.indica were significantly increased by 101.27%,102.41%,76.07%,278.23% and 265.52%,respectively.The fibrous root number,fibrous root length,underground fresh weight and dry weight were significantly increased by 52.57%,63.39%,23.65% and 32.52%,respectively,and the tuber diameter was increased by 12.14%.The activities of catalase(CAT)and peroxidase(POD) were significantly increased by 9.79% and 19.35%,respectively,and the activity of superoxide dismutase(SOD) was reduced by 28.02% compared to CK.The contents of soluble sugar and proline were significantly increased by 42.84% and 92.19%,respectively.Root activity was significantly increased by 65.57%.In summary,P.indica can promote the growth and enhance stress resistance of C.convolvulacea,and can be used to improve the cultivation quality of C.convolvulacea.

Key words: Codonopsis convolvulacea, Piriformospora indica, Medicinal plant, Growth and development, Stress resistance

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