关键词: 吡咯伯克霍尔德氏菌, 棉花黄萎病菌, 生物防治, 盆栽防效, 促生作用, 定殖动态

Abstract: Burkholderia pyrrocinia YZU-S377(S377) is a rice rhizosphere bacterium obtained in the early stage,which has broad-spectrum inhibitory effect on plant pathogens.To verify the biocontrol potential of S377 against Verticillium dahliae,the control effect of S377 on cotton Verticillium wilt,the colonization dynamics and growth promoting effect of S377 on cotton were tested in pot. The results showed that the relative inhibition rate of the strain to V.dahliae was 89.22%,and the control effect of pot test was 62.53%.After treated with S377 sterile filtrate the germination rate of cotton increased by 22.22 percentage points,the root length of cotton seedlings increased by 27.78 mm,and the fresh weight of aboveground part increased by 0.73 g.The results of crystal violet staining showed that strain S377 had the ability to form biofilm.The biofilm formation intensity of strain S377 at OD₆₀₀=0.15 and 0.25 was significantly more obvious than that under other concentrations.The colonization experiment showed that S377 could colonize cotton roots for 21 days,and the number increased first and then decreased. The
results indicated that S377 was a potential biocontrol strain against cotton Verticillium wilt.

Key words: Burkholderia pyrrocinia;Verticillium dahliae;Biological control;Pot experiment;Growth promotion, Colonization dynamics