河南农业科学 ›› 2022, Vol. 51 ›› Issue (4): 11-21.DOI: 10.15933/j.cnki.1004-3268.2022.04.002
赵志祥,严婉荣,王宝,何舒,曾向萍,肖彤斌
收稿日期:
2021-10-26
出版日期:
2022-04-15
发布日期:
2022-06-30
通讯作者:
肖彤斌(1972-),女,海南海口人,研究员,硕士,主要从事植物病虫害综合防控研究。E-mail:Xiaotbin@sina.com
作者简介:
赵志祥(1981-),男,湖南岳阳人,副研究员,博士,主要从事植物病害生物防治研究。E-mail:zhaozhixiang0207@126.com
基金资助:
ZHAO Zhixiang,YAN Wanrong,WANG Bao,HE Shu,ZENG Xiangping,XIAO Tongbin
Received:
2021-10-26
Published:
2022-04-15
Online:
2022-06-30
摘要: 辣椒枯萎病是由尖孢镰刀菌(Fusarium oxysporum)引起的严重制约辣椒生产的土传病害之一,目前尚缺乏有效的抗病材料,而化学药剂则不能完全根治,且易造成环境污染、农药残留及病菌抗药性等问题。利用微生物及其代谢产物、植物提取物等生物农药进行防治,既能控制病害的发生,又不会破坏生态环境、危害人畜健康,符合农业可持续发展的要求。综述了近20 a来国内外用于辣椒枯萎病生物防治的微生物种类、植物来源及其生防机制研究进展,分析了目前生防产品使用中存在的问题,并对辣椒枯萎病生物防治的未来发展方向进行了展望。
中图分类号:
赵志祥, 严婉荣, 王宝, 何舒, 曾向萍, 肖彤斌. 辣椒枯萎病生物防治研究进展[J]. 河南农业科学, 2022, 51(4): 11-21.
ZHAO Zhixiang, YAN Wanrong, WANG Bao, HE Shu, ZENG Xiangping, XIAO Tongbin. Research Advances in Biological Control of Pepper Fusarium Wilt[J]. Journal of Henan Agricultural Sciences, 2022, 51(4): 11-21.
[1]ATTRI K,SHARMA M,GUPTE S K.Influence of edaphic factors on Fusarium wilt of bell pepper[J].International Journal of Bio‑resource and Stress Management,2018,9(5):606‑610. [2]俞大绂.中国镰刀菌属菌种的初步名录[J].植物病理学报,1955,1(1):1‑17. YU D F.A preliminary list of strains of Fusarium in China[J].Acta Phytopathologica Sinica,1955,1(1):1‑17. [3]MALLY N,RIVERA J,HILDA A,et al.Phylogenetics and histology provide insight into damping‑off infections of‘Poblano’pepper seedlings caused by Fusarium wilt in greenhouses[J].Mycological Progress,2018,17(11):1237‑1249. [4]MOHAMMED T A,WELDERUFAEL A H,YESHINIGUS B B.Assessment and distribution of foliar and soil‑borne diseases of Capsicum species in Ethiopia [J].International Journal of Phytopathology,2021,10(2):125‑139. [5]BASHIR M R,ATIQ M,SAJID M,et al.Antifungal exploitation of fungicides against Fusarium oxysporum f.sp.capsici causing Fusarium wilt of chilli pepper in Pakistan[J].Environmental Science and Pollution Research,2018,25(7):6797‑6801. [6]EL‑ERAKY A M,MOUBASHER A H,ISMAIL M A,et al.Mycosynthesis of silver nanoparticles and their role in the control of Fusarium wilt of pepper[J].Journal of Basic & Applied Mycology(Egypt),2017,8(1):25‑34. [7]WANG J H,WANG S X,ZHAO Z,et al.Species composition and toxigenic potential of Fusarium isolates causing fruit rot of sweet pepper in China[J].Toxins,2019,11(12):690. [8]GABREKIRISTOS E,DEMIYO T. Hot pepper Fusarium wilt(Fusarium oxysporum f.sp.capsici):Epidemics,characteristic features and management options[J].Journal of Agricultural Science,2020,12(10):347‑360. [9]HEWEDY O A,ABDEL‑LATEIF K S,BAKR R A.Genetic diversity and biocontrol efficacy of indigenous Trichoderma isolates against Fusarium wilt of pepper[J].Journal of Basic Microbiology,2020,60(2):126‑135. [10]SAHI I Y,KHALID A N.In vitro biological control of Fusarium oxysporum causing wilt in Capsicum annum[J].Mycopathology,2007,5(2):85‑88. [11]BHAT M N,MESTA R,YENJERAPPA S T,et al.Biological control of Fusarium wilt of chillies using Trichoderma spp.[J].Indian Journal of Horticulture,2016,73(1):74‑77. [12]DAR G H,MIR G H,RASHID H,et al.Evaluation of microbial antagonists for the management of wilt/root rot and damping‑off diseases in chilli(Capsicum annuum)[J].Vegetos,2015,28(4):102‑110. [13]REPALLE S,KRISHNA M S R. Antimycotic effect of Trichoderma species on Fusarium oxysporum f.sp.capsici inciting vascular wilt in chilli[M]//VISWANATH B,INDRAVATHI G. New horizons in biotechnology. California:Paramount Publishing House,2015:29‑31. [14]JAMIOLKOWSKA A, MICHALEK W.Effect of mycorrhiza inoculation of pepper seedlings(Capsicum annuum L.) on the growth and protection against Fusarium oxysporum infection[J].Acta Scientiarum Polonorum Hortorum Cultus,2019,18(1):161‑169. [15]AL‑MORAD N Y,IBRAHEEM B Y,AL‑MORAD N Y M.Influence of Glomus intraradices and Trichoderma harzianum on pepper Fusarium wilt control[J].Tikrit Journal for Agricultural Sciences,2018,18(3):128‑136. [16]JALALULDEEN A F,SIJAM K,RAMADAN N A.Active changes of lignifications‑related enzymes in chili pepper response to Glomus mosseae against Fusarium oxysporum[J].Australian Journal of Basic and Applied Sciences,2020,14(6):1‑6. [17]KUMAR M,ASHWIN R,BAGYARAJ D J.Screening arbuscular mycorrhizal fungi in order to select the best for alleviating wilt disease complex of Capsicum[J].Proceedings of the National Academy of Sciences,2018,88(2):679‑684. [18]SAJEENA A, NAIR D S,SREEPAVAN K.Non‑pathogenic Fusarium oxysporum as a biocontrol agent[J].Indian Phytopathology,2020,73(2) :177‑183. [19]AL‑ANI L K T.Secondary metabolites of non‑pathogenic Fusarium:Scope in agriculture[M]//SINGH H B,KESWANI C,REDDY M S,et al.Secondary metabolites of plant growth promoting rhizomicroorganisms. Singapore:Springer,2019:59‑76. [20]詹洪.尖抱擁刀菌多态性及枯萎病植物疫苗工程菌的研究[D].福州:福建农林大学,2013:23‑44. ZHAN H.Studies on polymorphism of Fusarium oxysporum and its non‑pathogenic strain as plant vaccine against Fusarium wilt[D].Fuzhou:Fujian Agriculture and Forestry University,2013:23‑44. [21]VELOSO J,DIAZ J. The non‑pathogenic Fusarium oxysporum Fo47 induces distinct responses in two closely related solanaceae plants against the pathogen Verticillium dahliae[J].Journal of Fungi,2021,7(5):344. [22]JOSHI M,SRIVASTAVA R,SHARMA A K,et al.Screening of resistant varieties and antagonistic Fusarium oxysporum for biocontrol of Fusarium wilt of chilli[J].Journal of Plant Pathology & Microbiology,2012,3(5):134‑139. [23]MMBAGA M T,GURUNG S,MAHESHWARI A.Screening of plant endophytes as biological control agents against root rot pathogens of pepper(Capsicum annum L.)[J].Journal of Plant Pathology &Microbiology,2018,9(3):435. [24]EL‑KICHAOUI A,ELNABRIS K,SHAFIE A,et al.Development of Beauveria bassiana based bio‑fungicide against Fusarium wilt pathogens for Capsicum annuum,a promising approach toward vital biocontrol industry in Gaza strip[J].IUG Journal of Natural Studies,2017,25(2):183‑190. [25]JABER L R, ALANANBEH K M.Fungal entomopathogens as endophytes reduce several species of Fusarium causing crown and root rot in sweet pepper(Capsicum annuum L.)[J].Biological Control,2018,126:117‑126. [26]卯婷婷,陶刚,赵玳琳,等.一株粪生毛壳菌的分离鉴定及其对辣椒枯萎病的防治效果[J].中国生物防治学报,2017,33(4):552‑560. MAO T T,TAO G,ZHAO D L,et al.Isolation and identification of Coprophilous chartomium strain and its biocontrol effect against pepper Fusarium wilt[J].Chinese Journal of Biological Control,2017,33(4):552‑560. [27]莫维弟,卯婷婷.辣椒枯萎病土壤拮抗真菌的筛选与鉴定[J].贵州农业科学,2019,47(8):66‑70. MO W D,MAO T T.Screening and identification of antagonistic fungi in soil against Fusarium oxysporum in pepper[J].Guizhou Agricultural sciences,2019,47(8):66‑70. [28]ABADA K A,AHMED M A. Management Fusarium wilt of sweet pepper by Bacillus strains[J].American Journal of Life Sciences,2014,2(6):19‑25. [29]YANTI Y,WARNITA W,REFLIN R,et al.Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wilt disease on chili pepper [J].Biodiversitas,2018,19(4):1532‑1538. [30]CHOWDHURY S K,MAJUMDAR S,MANDAL V.Application of Bacillus sp.LBF‑01 in Capsicum annuum plant reduces the fungicide use against Fusarium oxysporum[J].Biocatalysis and Agricultural Biotechnology,2020,27:101714. [31]MILJAKOVIC D,MARINKOVIC J,BALESEVIC‑TUBIC S.The significance of Bacillus spp. indisease suppression and growth promotion of field and vegetable crops[J].Microorganisms,2020,8(7):1037. [32]YU X,AI C,XIN L,et al.The siderophore‑producing bacterium,Bacillus subtilis CAS15,has a biocontrol effect on Fusarium wilt and promotes the growth of pepper[J].European Journal of Soil Biology,2011,47(2):138‑145. [33]WU Z S,HUANG Y Y,LI Y,et al.Biocontrol of Rhizoctonia solani via induction of the defense mechanism and antimicrobial compounds produced by Bacillus subtilis SL‑44 on pepper (Capsicum annuum L.)[J].Frontiers in Microbiology,2019,10:2676. [34]WU Y,ZHAO C,FARMER J,et al.Effects of bio‑organic fertilizer on pepper growth and Fusarium wilt biocontrol[J].Scientia Horticulturae,2015,193:114‑120. [35]杨吉春,王帅印,刘长令.2019年全球登记或上市的农药品种[J].农药,2020,59(4):292‑295,302. YANG J C,WANG S Y,LIU C L.Globally registered or listed pesticide varieties in 2019[J].Agrochemicals, 2020,59(4):292‑295,302. [36]SARAVANAKUMAR D,THOMAS A,BANWARIE N.Antagonistic potential of lipopeptide producing Bacillus amyloliquefaciens against major vegetable pathogens[J].European Journal of Plant Pathology,2019,154(2):319‑335. [37]KANG B R,PARK J S,JUNG W J.Antifungal evaluation of fengycin isoforms isolated from Bacillus amyloliquefaciens PPL against Fusarium oxysporum f.sp.lycopersici[J].Microbial Pathogenesis,2020,149:104509. [38]ZHAO J,WANG Y G,LIANG H,et al.The rhizosphere microbial community response to a bio‑organic fertilizer: Finding the mechanisms behind the suppression of termelon Fusarium wilt disease[J].Acta Physiologiae Plantarum,2018,40(1):1‑14. [39]BASCO M J,BISEN K,KESWANI C,et al.Biological management of Fusarium wilt of tomato using biofortified vermicompost[J].Mycosphere,2017,8(3):467‑483. [40]SACHDEV S,SINGH R P.Isolation,characterisation and screening of native microbial isolates for biocontrol of fungal pathogens of tomato[J].Climate Change and Environmental Sustainability,2018,6(1):46‑58. [41]SUNDARAMOORTHY S,BALABASKAR P.Interactions between Fluorescent pseudomonads and chilli(Capsicum annuum L.) plants[J].Research &Reviews in Biotechnology & Biosciences,2017,4(1):27‑30. [42]李䶮,刘娜,郑丽博.荧光假单胞菌植物病害防治及研究进展[J].分子植物育种,2018,16(11):3693‑3697. LI Y,LIU N,ZHENG L B.Prevention and treatment of plant diseases of Pseudomonas fluorescens and research progress[J].Molecular Plant Breeding,2018,16(11):3693‑3697. [43]MAMPHOGORO T P.Bacterial communities associated with the surface of sweet pepper and their selection for biocontrol[D].Potchefstroom:North‑West University,2021. [44]JUNG B K,HONG S J,PARK G S,et al.Isolation of Burkholderia cepacia JBK9 with plant growth‑promoting activity while producing pyrrolnitrin antagonistic to plant fungal diseases[J].Applied Biological Chemistry,2018,61(2):173‑180. [45]HERDYASTUTI N,FAUZIAH R W,PRABOWO Y Y,et al.Diversity of chitinolytic bacteria from shrimp farms and their antifungal activity[J].Journal of Biosciences,2021,12(3):55‑68. [46]SURYANTO D,BUNGSU A,TANIWAN S,et al.Application of a single and combination of plant‑growth promoting bacteria and actinomycete to increase chili(Capsicum annuum L.) health and growth[J].Journal of Pure and Applied Microbiology,2017,11(3):1379‑1386. [47]SAOUSSEN B K,BOUTHEINA M T,SLIM T.Biological potential of Bacillus subtilis V26 for the control of Fusarium wilt and tuber dry rot on potato caused by Fusarium species and the promotion of plant growth[J].Biological Control,2020,152:10444. [48]HUTCHINGS M I,TRUMAN A W,WILKINSON B.Antibiotics:Past,present and future[J].Current Opinion in Microbiology,2019,51(1):72‑80. [49]HE H,HAO X,ZHOU W,et al.Identification of antimicrobial metabolites produced by a potential biocontrol Actinomycete strain A217[J].Journal of Applied Microbiology,2020,128(4):1143‑1152. [50]LIOTTI R G,FIGUEIREDO M I,SOARES M A.Streptomyces griseocarneus R132 controls phytopathogens and promotes growth of pepper(Capsicum annuum)[J].Biological Control,2019,138:104065. [51]钱瑶,潘园园,李二伟,等.链霉菌IMS002的分类鉴定及其抗真菌活性物质解析[J].微生物学报,2020,60(1):60‑68. QIAN Y,PAN Y Y,LI E W,et al.Classification of Streptomyces sp. IMS002 and identification of its antifungal metabolite[J].Acta Microbiologica Sinica,2020,60(1):60‑68. [52]JAVAID A,RAUF S.Management of basal rot disease of onion with dry leaf biomass of Chenopodium album as soil amendment[J].International Journal of Agriculture and Biology,2015,17(1):142‑148. [53]ZAHEER Z,SHAFIQUE S,SHAFIQUE S,et al.Antifungal potential of Parthenium hystero-phorus L.plant extracts against Fusarium solani[J].Scientific Research Essays,2012,7(22):2049‑2054. [54]SHAFIQUE S,ASIF M,SHAFIQUE S.Management of Fusarium oxysporum f.sp.capsici by leaf extracts of Eucalyptus citriodora[J].Pakistan Journal of Botany,2015,47(3):1177‑1182. [55]SINGH J K,KUMAR M,KUMAR S,et al.Inhibitory effect of botanicals on growth and sporulation of Fusarium oxysporum inciting wilt of chilli(Capsicum annuum L.)[J].Journal of Pharmacognosy and Phytochemistry,2017,6(5):2199‑2204. [56]SUPRAPTA D N,KHALIMI K.Anti‑fungal activities of selected tropical plants from Bali Island[J].Phytopharmacology,2012,2(2):265‑270. [57]JAMIL A,MUSHEER N,ASHRAF S.Antagonistic potential of Trichoderma harzianum and Azadirachta indica against Fusarium oxysporum f. sp. capsici for the management of chilli wilt[J].Journal of Plant Diseases and Protection,2021,128(1):161‑172. [58]JAMA Q,LEE Y S,JEON H D,et al.Effect of plant growth‑promoting bacteria Bacillus amyloliquefaciens Y1 on soil properties,pepper seedling growth,rhizosphere bacterial flora and soil enzymes[J].PlantProtection Science,2018,54(3):129‑137. [59]MAO T,JIANG X.Changes in microbial community and enzyme activity in soil under continuous pepper cropping in response to Trichoderma hamatum MHT1134 application[J].Scientific Reports,2021,11(1):1‑12. [60]MA H Y,YANG B,WANG H W,et al.Application of Serratia marcescens RZ‑21 significantly enhances peanut yield and remediates continuously cropped peanut soil[J].Journal of the Science of Food and Agriculture,2016,96(1):245‑253. [61]CAO Y,PI H,CHANDRANGSU P,et al.Antagonism of two plant‑growth promoting Bacillus velezensis isolates against Ralstonia solanacearum and Fusarium oxysporum[J].Scientific Report,2018,8(1):1‑14. [62]郭珺,武爱莲,闫敏,等.芽孢杆菌Pb-4菌株鉴定及其抑菌活性的研究[J].华北农学报,2016,31(2):224‑230. GUO J,WU A L,YAN M,et al.Identification of Bacillus sp.Pb‑4 and its unitilization for antimicrobial activity[J].Acta Agriculturae Boreali‑Sinica,2016,31(2):224‑230. [63]LIANG C,ZANG C,MARK I,et al.Two imide substances from a soil‑isolated Streptomyces atratus strain provide effective biocontrol activity against grapevine downy mildew[J].Biocontrol Science and Technology,2016,26(10):1337‑1351. [64]DANIEL H C F,WILFREDO F F,FRANCISCO C R,et al.Antibiosis in vitro of Trichoderma strains metabolic extract on mycelial growth and reproductive capacity of Fusarium oxysporum isolated from pepper plants(Capsicum annuum L.)[J]. British Biotechnology Journal,2014,4(4):387‑399. [65]NAWROCKA J,MALOLEPSZA U,SZYMCZAK K,et al.Involvement of metabolic components,volatile compounds,proteins,and mechanical strengthening in multilayer protection of cucumber plants against Rhizoctonia solani activated by Trichoderma atroviride TRS25[J].Protoplasma,2018,255(1):359‑373. [66]LIU K,MCLNROY J A,HU C H,et al.Mixtures of plant‑growth‑promoting rhizobacteria enhance biological control of multiple plant diseases and plant‑growth promotion in the presence of pathogens[J].Plant Disease,2018,102(1):67‑72. [67]MAHMOUD M A. Efficiency of some bioagents and nemastop compound in controlling damping off and root rot diseases on peanut plants[J].International Journal of Advanced Research in Biological Sciences,2015,2(11):77‑86. [68]AFZAL I,IQRAR I,SHINWARI Z K,et al.Plant growth promoting potential of endophytic bacteria isolated from roots of wild Dodonaea viscosa L.[J].Plant Growth Regul,2017,81(3):399‑408. [69]WELLER D M,MAVRODI D V,PELT J A,et al.Induced systemic resistance in Arabidopsis thaliana against Pseudomonas syringae pv. tomato by 2,4‑diacetylphloroglucinol producing Pseudomonas fluorescens[J].Phytopathology,2012,102(4):403‑412. [70]HAFSAH S,RAHAYU A,SYAMSUDDIN S,et al.Induced resistance against Fusarium wilt disease caused by Fusarium oxysporum in local red peppers(Capsicum annum L.) in Aceh using rhizobacteria isolates[J].Journal of Tropical Horticulture,2019,2(1):6‑10. [71]PHI Q T,PARK Y M,SEUL K J,et al.Assessment of root‑associated Paenibacillus polymyxa groups on growth promotion and induced systemic resistance in pepper[J].Journal of Microbiology Biotechnology,2010,20(12):1605‑1613. [72]JAYAPALA N,MALLIKARJUNAIAH N,PUTTASWAMY H,et al.Rhizobacteria Bacillus spp.induce resistance against anthracnose disease in chili(Capsicum annuum L. )through activating host defense response[J].Egyptian Journal of Biological Pest Control,2019,29(1):1‑9. |
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