河南农业科学 ›› 2020, Vol. 49 ›› Issue (3): 63-69.DOI: 10.15933/j.cnki.1004-3268.2020.03.009

所属专题: 中草药种子引发专题

• 作物栽培·遗传育种 • 上一篇    下一篇

水杨酸浸种处理对藏药甘青青兰种子萌发及幼苗抗旱性的影响

禄亚洲1,2,3,李宁3,张二豪2,3,尹秀2,3,赵垦田4,兰小中2,3   

  1. (1.西藏农牧学院 高原生态研究所,西藏 林芝 860000;2.西藏农牧学院-西南大学药用植物联合研发中心,西藏 林芝 860000;3.西藏农牧学院 食品科学学院,西藏 林芝 860000;4.西藏农牧学院 资源与环境学院,西藏 林芝 860000)
  • 收稿日期:2019-08-19 出版日期:2020-03-15 发布日期:2020-03-15
  • 通讯作者: 兰小中(1973-),男,四川达州人,教授,博士,主要从事药用植物分子生物学与生理学研究。E-mail:lanxiaozhong@163.com
  • 作者简介:禄亚洲(1987-),男,甘肃白银人,讲师,在读博士研究生,研究方向:药用植物分子生物学。E-mail:luyazhou001@126.com
  • 基金资助:
    中央本级重大增减支项目(2060302);西藏自治区科技计划项目科技重大专项(XZ201901-GA-04);西藏自治区科学技术厅科学研究项目(XZ2017ZRG-22)

Effects of Soaking Seeds with Salicylic Acid on Seed Germination and Drought Resistance of Dracocephalum tanguticum

LU Yazhou1,2,3,LI Ning3,ZHANG Erhao2,3,YIN Xiu2,3,ZHAO Kentian4,LAN Xiaozhong2,3   

  1. (1.Research Institute of Plateau Ecology,Tibet Agriculture and Animal Husbandry University,Nyingchi 860000,China;2.Tibet Agriculture and Animal Husbandry University-Southwest University Medicinal Plants Joint Research and Development Center,Nyingchi 860000,China;3.Food Science University,Tibet Agriculture and Animal Husbandry University,Nyingchi
    860000,China;4.Department of Resources and Environment,Tibet Agriculture and Animal Husbandry University,Nyingchi 860000,China)
  • Received:2019-08-19 Published:2020-03-15 Online:2020-03-15

摘要: 为了探讨外源水杨酸对干旱胁迫下甘青青兰(Dracocephalum tanguticum)种子萌发及幼苗抗旱能力的影响,采用PEG-6000模拟干旱胁迫,以不同浓度外源水杨酸(0、0.2、0.4、0.6、0.8 mmol/L)对藏药甘青青兰进行浸种 24 h处理,研究不同浓度SA对甘青青兰种子萌发及幼苗抗旱性的影响。结果表明,0.6 mmol/L SA浸种甘青青兰种子萌发效果最好,种子发芽率、发芽势、发芽指数和活力指数较CK(蒸馏水处理)分别提高了34.82%、34.42%、34.87%和54.86%;干旱胁迫(20% PEG-6000模拟干旱)下,与CK相比,0.6 mmol/L SA浸种显著提高了甘青青兰幼苗叶片中脯氨酸含量,降低丙二醛含量,但幼苗叶片中叶绿素含量和可溶性蛋白含量与CK无显著差异。说明外源水杨酸浸种可通过提高种子发芽势、发芽指数和活力指数,进而促进种子萌发,并增强植物抗渗透胁迫能力,降低细胞膜脂过氧化损伤,提高甘青青兰幼苗的抗旱能力,且0.6 mmol/L SA浸种24 h效果最佳。

关键词: 甘青青兰, 水杨酸, 萌发特性, 水分胁迫, 抗旱性, 浸种

Abstract: In order to investigate the effects of exogenous salicylic acid on germination and drought stress of Dracocephalum tanguticum,different concentrations of SA(0,0.2,0.4,0.6,0.8 mmol/L) were used to soak the seeds of D.tanguticum,and then the seed germination and seedling physiological characteristics of D.tanguticum were studied under 20% PEG-6000 simulated drought stress.The results showed that 0.6 mmol/L SA had the best effect on germination by soaking seed,the germination rate,germination energy,germination exponent and vigor index were increased by 34.82%,34.42%,34.87% and 54.86% respectively compared with CK;Under drought stress,the exogenous SA increased the content of proline and decreased the malondialdehyde content at the concentration of 0.6 mmol/L,however,the chlorophyll and soluble protein contents were similar compared with CK.In conclusion,exogenous SA could improve the seed germination by promoting germination energy,germination exponent and vigor index,and increase the ability of D.tanguticum to resist drought stress by enhancing the ability of plants to resist osmotic stress,reducing the cell membrane damage,and 0. 6 mmol/L SA soaking for 24 h was the best.

Key words: Dracocephalum tanguticum, Salicylic acid, Germination characteristics, Water stress;Drought resistance, Soaking seeds

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