猪传染性胸膜肺炎放线杆菌氨基糖苷类抗生素耐药基因的检测

doi:10.15933/j.cnki.1004-3268.2020.11.018

河南农业科学 ›› 2020, Vol. 49 ›› Issue (11): 141-146.DOI: 10.15933/j.cnki.1004-3268.2020.11.018

猪传染性胸膜肺炎放线杆菌氨基糖苷类抗生素耐药基因的检测

李海利¹，冯丽丽²，王英华³，徐引弟¹，张青娴¹，王治方¹，朱文豪¹，许峰¹，游一¹，王克领¹

（1.河南省农业科学院畜牧兽医研究所，河南郑州 450002；2.河南省农业科学院农业经济与信息研究所,河南郑州 450002; 3.河南省动物疫病预防控制中心，河南郑州 450008）

收稿日期:2020-04-11 出版日期:2020-11-15 发布日期:2020-11-15
通讯作者: 李海利（1982-），男，河南开封人，副研究员，博士，主要从事动物疫病临床诊断及防控研究。E-mail:haili8693@sina.com
作者简介:李海利（1982-），男，河南开封人，副研究员，博士，主要从事动物疫病临床诊断及防控研究。E-mail:haili8693@sina.com
基金资助:
河南省科技攻关（国际科技合作）项目（182102410038）

Detection of Aminoglycoside Antibiotic Resistance Genes in Pig Infectious Porcine Contagious Pleuropneumonia Actinobacillus pleuropneumoniae Isolated from Pigs

LI Haili¹,FENG Lili²,WANG Yinghua³,XU Yindi¹,ZHANG Qingxian¹,WANG Zhifang¹,ZHU Wenhao¹,XU Feng¹,YOU Yi¹,WANG Keling¹

(1.Animal Husbandry and Veterinary Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Institute of Agricultural Economics and Information,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;3.Henan Provincial Center for Animal Disease Control and Prevention,Zhengzhou 450008,China)

Received:2020-04-11 Published:2020-11-15 Online:2020-11-15

摘要/Abstract

摘要： 为研究河南省及临近周边地区的规模化猪场猪传染性胸膜肺炎（Porcine contagious pleuropneumonia）放线杆菌（Actinobacillus pleuropneumoniae，APP）氨基糖苷类抗生素药物的耐药情况，调查了河南省及周边地区56个规模化猪场,对猪的肺脏和气管中分离鉴定的85株APP进行了研究。血清型鉴定结果显示，分离菌株为1、3、7、8、9、10、12、15型APP，采用纸片扩散法对分离株进行了耐药表型鉴定和采用PCR方法对氨基糖苷类抗生素药物耐药基因（aac3-Ⅳ、aac3-Ⅱc、ant2-Ⅰa）进行了检测。药敏试验结果显示，APP对氨基糖苷类抗生素药物耐药基因（aac3-Ⅱc）和（aac3-Ⅳ）的耐药率分别为57.6%（49/85）、81.2%（69/85）；未检测出ant2-Ⅰa耐药基因。

关键词: 猪, 胸膜肺炎放线杆菌；血清定型；氨基糖苷类抗生素；耐药表型；耐药基因

Abstract: In order to study the aminoglycoside antibiotic resistance of pig infectious porcine contagious pleuropneumonia Actinobacillus pleuropneumoniae(APP) in Henan Province and neighboring regions in the scale of swine.A drug sensitivity test was carried out in 85 APP strains isolated from lung and trachea pigs in 56 large scale pig farms in Henan Province.The serotype identification results showed that isolates were 1,3,7,8,9,10,12,15.Antimicrobial susceptibility disk method were used for resistant phenotype identification.At the same time,the PCR method for detection of aminoglycoside antibiotic resistance genes(ant2-Ⅰ a,aac3-Ⅱ c and aac3-Ⅳ).The drug resistance rates of APP to aminoglycoside antibiotic drug resistance genes(aac3-Ⅱ c)and(aac3-Ⅳ) were 57.6% (49/85) and 81.2% (69/85),respectively.The ant2-Ⅰ a resistance genes were not detected.

Key words: Swine, Actinobacillus pleuropneumoniae, Serum stereotyping, Aminoglycoside antibiotic, Drug resistance phenotype, Resistant gene

中图分类号:

S855

李海利, 冯丽丽, 王英华, 徐引弟, 张青娴, 王治方, 朱文豪, 许峰, 游一, 王克领. 猪传染性胸膜肺炎放线杆菌氨基糖苷类抗生素耐药基因的检测[J]. 河南农业科学, 2020, 49(11): 141-146.

LI Haili, FENG Lili, WANG Yinghua, XU Yindi, ZHANG Qingxian, WANG Zhifang, ZHU Wenhao, XU Feng, YOU Yi, WANG Keling. Detection of Aminoglycoside Antibiotic Resistance Genes in Pig Infectious Porcine Contagious Pleuropneumonia Actinobacillus pleuropneumoniae Isolated from Pigs[J]. Journal of Henan Agricultural Sciences, 2020, 49(11): 141-146.

猪传染性胸膜肺炎放线杆菌氨基糖苷类抗生素耐药基因的检测

Detection of Aminoglycoside Antibiotic Resistance Genes in Pig Infectious Porcine Contagious Pleuropneumonia Actinobacillus pleuropneumoniae Isolated from Pigs

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