河南农业科学 ›› 2019, Vol. 48 ›› Issue (9): 54-59.DOI: 10.15933/j.cnki.1004-3268.2019.09.008

所属专题: 中草药种子引发专题

• 作物栽培·遗传育种 • 上一篇    下一篇

干旱胁迫下种子引发对板蓝根种子萌发及幼苗生长的影响

孟衡玲,卢丙越,苏一兰,王田涛,张 薇   

  1. (红河学院/云南省高校农作物优质高效栽培与安全控制重点实验室,云南 红河 661100)
  • 收稿日期:2019-05-02 出版日期:2019-09-15 发布日期:2019-09-15
  • 通讯作者: 张 薇(1977-),女,云南泸西人,副教授,博士,主要从事药用植物资源开发与利用研究。E-mail:zw_biology2@126.com
  • 作者简介:孟衡玲(1981-),女,云南宣威人,讲师,博士,主要从事药用植物资源开发与利用研究。E-mail:menghengl@163.com
  • 基金资助:
    云南省中青年学术带头人后备人才项目(2016PY068);红河学院学术带头人后备人才资助项目(2015HB0303)

 Effects of Seed Priming on Germination and Seedling Growth of Radix Isatidis under Drought Stress

MENG Hengling,LU Bingyue,SU Yilan,WANG Tiantao,ZHANG Wei   

  1.  (Honghe University/The Key Laboratory of High Quality Crops Cultivation and Safety Control of Yunnan Province,Honghe 661100,China)
  • Received:2019-05-02 Published:2019-09-15 Online:2019-09-15

摘要:

为筛选适宜的板蓝根种子引发剂,采用PEG-6000模拟干旱胁迫,以PEG-6000、CaCl2、GA3、水 4种不同引发剂对板蓝根种子进行引发处理,探讨板蓝根种子在干旱胁迫下的发芽特性及幼苗的生长状况。结果表明,干旱胁迫(20% PEG-6000模拟干旱)下,4种引发剂均能不同程度地提高板蓝根种子的发芽率、发芽势和发芽指数。综合比较来看,18 h水引发处理效果最好,种子发芽率、发芽势、发芽指数较CK2(干旱胁迫但未进行引发)分别提高33.3%、36.6%、106.4%。其次是25% PEG-6000引发处理,种子发芽率、发芽势、发芽指数较CK2分别提高35.2%、51.2%、32.8%;50 mg/L GA3引发处理下的种子发芽指数显著高于其他处理,较CK2提高171.1%,可有效提高种子的发芽速率;1.5% CaCl2处理引发效果最差,种子发芽率、发芽势和发芽指数较CK2分别提高16.7%、19.5%和23.9%。种子经过引发后,可不同程度提高板蓝根幼苗的株高、根长、叶绿素含量,降低叶片的相对电导率。综上,4种不同引发剂对板蓝根种子的引发效果依次为18 h水>25% PEG-6000>50 mg/L GA3>1.5% CaCl2,建议在板蓝根种子播种前进行18 h水引发处理。

关键词: 板蓝根, 种子引发, 干旱胁迫, 渗调引发, 激素引发, 水引发, 种子萌发

Abstract:  In order to screen out the appropriate seed initiator for Radix Isatidis,four initiators including PEG-6000,CaCl2,GA3 and H2O were used to treat the Radix Isatidis seeds,and then the seed germination and seedling growth characteristics of Radix Isatidis were investigated under 20% PEG-6000 simulated drought stress.The results showed that all these four initiators could improve seed germination rate, germination potential and germination index in different degrees under drought stress.The 18 h water priming had the best effect on seed germination,and the seed germination rate,germination potential,and germination index were increased by 33.3%,36.6% and 106.4% respectively compared with CK2(drought stress and no initiators).The follower was 25% PEG-6000 priming,and the germination rate,germination potential,germination index were 35.2%,51.2%,and 32.8% higher than CK2,respectively.The 50 mg/L GA3 priming had a significantly higher effect than other treatments on germination index,which was increased by 171.1% compared with CK2.The 1.5% CaCl2 had lowest effect on germination rate, germination potential and germination index,which only were increased by 16.7%,19.5% and 23.9% respectively compared with CK2.In addition,after seed priming,the plant height,root length,chlorophyll content of seedlings were significantly increased,and the relative conductivity was decreased.In conclusion,the seed priming effect was in turns: 18 h water >25% PEG-6000 >50 mg/L GA3 >1.5% CaCl2 ,which suggested that 18 h water priming to treat the seeds before sowing could greatly improve the seed germination and seedling growth of Radix Isatidis

Key words: Radix Isatidis; Seed priming, Drought stress, Osmotica priming, Hormone priming, Water priming, Seed germination

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