河南农业科学 ›› 2023, Vol. 52 ›› Issue (10): 22-29.DOI: 10.15933/j.cnki.1004-3268.2023.10.003

• 作物栽培·遗传育种 • 上一篇    下一篇

衣藻对盐胁迫下小麦幼苗光合生理指标的影响

王彦钦1,孟宪刚1,李武阳1,罗光宏2   

  1. (1.兰州交通大学生物与制药工程学院,甘肃 兰州 730070;2.河西学院甘肃省微藻工程技术研究中心,甘肃 张掖 734000)
  • 收稿日期:2023-02-28 出版日期:2023-10-15 发布日期:2023-11-08
  • 通讯作者: 孟宪刚(1974-),男,山西太谷人,教授,博士,主要从事工业微生物以及环境生物工程研究。E-mail:mengxg@mail.lzjtu.cn
  • 作者简介:王彦钦(1998-),男,甘肃兰州人,在读硕士研究生,研究方向:藻类生物肥料。E-mail:765663632@qq.com
  • 基金资助:
    甘肃省科技计划项目(18JR2JG001)

Effects of Chlamydomonas on Photosynthetic Physiological Indexes of Wheat Seedlings under Salt Stress

WANG Yanqin1,MENG Xiangang1,LI Wuyang1,LUO Guanghong2   

  1. (1.College of Biological and Pharmaceutical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;2.Microalgae Engineering Research Center of Gansu Province,Hexi University,Zhangye 734000,China)
  • Received:2023-02-28 Published:2023-10-15 Online:2023-11-08

摘要: 以正常生长的小麦幼苗为对照(CK),研究衣藻藻液[0 g/L(T0)、0.25 g/L(T1)、0.5 g/L(T2)、1.0 g/L(T3)和2.0 g/L(T4)]对盐胁迫下小麦幼苗叶片光合色素含量、光合气体交换参数以及叶绿素荧光参数等光合生理指标的影响,为衣藻在小麦盐胁迫下的应用提供理论依据。结果表明,CK小麦幼苗叶片光合色素含量、净光合速率(Pn)、气孔导度(Gs)、CO2浓度(Ci)、蒸腾速率(Tr)、光系统Ⅱ实际光量子产量[Y(Ⅱ)]、最大光化学效率(Fv/Fm)、电子传递速率(ETR)、光化学淬灭系数(qP)均最高,非光化学淬灭系数(qN)最低。T1—T4处理小麦幼苗叶片叶绿素a、总叶绿素和类胡萝卜素含量均高于T0处理,以T3、T4处理较高。T1—T4处理Pn、Gs、Ci、Tr在处理后6~12 d均高于T0处理,总体以T3处理最高,T4处理次之。T1—T4处理Fv/Fm、ETR、Y(Ⅱ)、qP在处理后6~12 d总体上均高于T0处理,以T3处理最高,T4处理次之;T1—T4处理qN在处理后6~12 d均低于T0处理。综合考虑,1.0 g/L衣藻藻液效果最佳。

关键词: 盐胁迫, 小麦, 衣藻藻粉, 光合作用, 光合生理指标, 叶绿素荧光参数

Abstract: The wheat seedlings under normal condition were as control(CK),the effects of Chlamydomonas algal liquid[0 g/L(T0),0.25 g/L(T1),0.5 g/L(T2),1.0 g/L(T3)and 2.0 g/L(T4)]on photosynthetic pigment content,photosynthetic gas exchange parameters and chlorophyll fluorescence parameters of wheat seedlings under high salt stress were investigated,so as to provide a theoretical basis for the application of Chlamydomonas algal liquid in wheat under salt stress.The results showed that wheat seedlings of CK had the highest photosynthetic pigment content,net photosynthetic rate(Pn),stomatal conductance(Gs),CO2 concentration(Ci),transpiration rate(Tr),actual optical quantum yield of optical system Ⅱ[Y(Ⅱ)],maximum photochemical efficiency(Fv/Fm),electron transport rate(ETR),photochemical quenching coefficient(qP),and the lowest non‑photochemical quenching coefficient(qN).The contents of chlorophyll a,total chlorophyll and carotenoids of T1—T4 treatments were higher than those in T0 treatment,especially T3 and T4 treatments.The Pn,Gs,Ci and Tr of T1—T4 treatments were higher than those of T0 treatment at 6—12 days after treatment,and T3 treatment was generally the highest,followed by T4 treatment.The Fv/Fm,ETR,Y(Ⅱ)and qP of T1—T4 treatments were overall higher than those of T0 treatment at 6—12 days after treatment,and T3 treatment was generally the highest,followed by T4 treatment;qN of T1—T4 treatments were lower than that of T0 treatment at 6—
12 days after treatment.In general,1.0 g/L Chlamydomonas algal liquid has the best effect.

Key words: Salt stress, Wheat, Chlamydomonas algal powder, Photosynthesis, Photosynthetic physiological indexes, Chlorophyll fluorescence parameters

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