河南农业科学 ›› 2023, Vol. 52 ›› Issue (9): 1-13.DOI: 10.15933/j.cnki.1004-3268.2023.09.001

• 综述 • 上一篇    下一篇

荧光标记技术在新型农用化合物创制中的应用现状及进展

文鼎1,张亚1,刘双清1,王翀2,廖晓兰1   

  1. (1.湖南农业大学植物保护学院,湖南 长沙 410128;2.湖南农业大学生物科技学院,湖南 长沙 410128)
  • 收稿日期:2022-12-12 出版日期:2023-09-15 发布日期:2023-10-10
  • 通讯作者: 张亚(1980-),男,湖南长沙人,副教授,博士,主要从事新型农用化合物创制研究。E-mail:zhangya@hunau.edu.cn 刘双清(1983-),男,湖南长沙人,副教授,博士,主要从事生防资源挖掘与利用研究。E-mail:liushuangqing@hunau.edu.cn
  • 作者简介:文鼎(1997-),男,湖南常德人,在读硕士研究生,研究方向:新型农用化合物作用机制。E-mail:wending@stu.hunau.edu.cn
  • 基金资助:
    国家自然科学基金面上项目(32072473);湖南省教育厅科学研究项目(21B0199)

Application Status and Progress of Fluorescent Labeling Technology in the Creation of Novel Agrocompounds

WEN Ding1,ZHANG Ya1,LIU Shuangqing1,WANG Chong2,LIAO Xiaolan1   

  1. (1.College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;2.College of Bioscience and
    Biotechnology,Hunan Agricultural University,Changsha 410128,China)
  • Received:2022-12-12 Published:2023-09-15 Online:2023-10-10

摘要: 荧光标记技术广泛应用于现代社会的各个方面,但其在农用化合物创制领域的应用鲜见综述。基于近10 a国内外农用化合物创制领域的荧光标记技术相关文献,系统总结了荧光标记技术中荧光染料的种类,综合介绍了荧光探针技术和免疫荧光技术在新型农用化合物创制领域的应用现状及进展,分析了目前荧光标记技术在新型农用化合物创制领域存在的局限与不足,并对未来荧光标记技术的发展趋势进行了展望。

关键词: 荧光标记, 农用化合物创制, 荧光染料, 荧光探针, 免疫荧光

Abstract: Fluorescent labeling technology(FLT)is widely used in all aspects of the modern society,but its application in the field of agrocompound creation has rarely been reviewed. Based on the domestic and foreign literature on FLT in the field of agrocompound creation in the past 10 years,the kinds of fluorescent dyes in FLT was introduced,the application situation and progress of fluorescence probe technology and immunofluorescence technology in new agrocompound creation field were summarized,the limitations and deficiencies of FLT in the field of agrocompound creation were analyzed,and the development trend of FLT in the future was prospected.

Key words: Fluorescent labeling, Agrocompound creation, Fluorescent dye, Fluorescent probe, Immunofluorescence

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