河南农业科学 ›› 2023, Vol. 52 ›› Issue (4): 1-8.DOI: 10.15933/j.cnki.1004-3268.2023.04.001
郝红燕,桑慧彤,吕山花,樊颖伦,李海云
收稿日期:
2022-11-08
出版日期:
2023-04-15
发布日期:
2023-05-12
通讯作者:
李海云(1974-),女,山东聊城人,副教授,博士,主要从事植物生理方面的教学与科研工作。E-mail:lhy040228@163.com
作者简介:
郝红燕(1999-),女,山东泰安人,在读硕士研究生,研究方向:园林植物种质资源研究与应用。E-mail:hhy2549517825@163.com
基金资助:
HAO Hongyan,SANG Huitong,LÜ Shanhua,FAN Yinglun,LI Haiyun
Received:
2022-11-08
Published:
2023-04-15
Online:
2023-05-12
摘要: 植物遭遇逆境会受到伤害,利用基因工程技术提高植物抗逆性是一条快捷有效的途径。碱性亮氨酸拉链(bZIP)转录因子是高等植物中数目较多且相对保守的基因家族之一,在植物抵御低温、干旱、盐渍、病虫等生物和非生物胁迫过程中具有重要的调控作用。综述了bZIP转录因子的结构、分类及其提高植物抗非生物胁迫(干旱、高温、低温、高盐、营养缺乏等)和生物胁迫的研究新进展,为bZIP 转录因子的应用及植物抗逆遗传改良提供参考。
中图分类号:
郝红燕, 桑慧彤, 吕山花, 樊颖伦, 李海云. bZIP 转录因子提高植物抗逆性研究新进展[J]. 河南农业科学, 2023, 52(4): 1-8.
HAO Hongyan, SANG Huitong, LÜ Shanhua, FAN Yinglun, LI Haiyun. New Research Progress of bZIP Transcription Factors in Improving Plant Stress Resistance[J]. Journal of Henan Agricultural Sciences, 2023, 52(4): 1-8.
[1]李志,薛姣,耿贵,等.逆境胁迫下甜菜生理特性的研究进展[J].中国农学通报,2021,37(24):39⁃47. LI Z,XUE J,GENG G,et al.The physiological characteristics of beets under stress:Research progress[J].Chinese Agricultural Science Bulletin,2021,37(24):39⁃47. [2]REN B Z,ZHANG J W,LI X,et al.Effects of waterlogging on the yield and growth of summer maize under field conditions[J].Canadian Journal of Plant Science,2014,94(1):23⁃31. [3]马富举,李丹丹,蔡剑,等.干旱胁迫对小麦幼苗根系生长和叶片光合作用的影响[J].应用生态学报,2012,23(3):724⁃730. MA F J,LI D D,CAI J,et al.Responses of wheat seedlings root growth and leaf photosynthesis to drought stress[J].Chinese Journal of Applied Ecology,2012,23(3):724⁃730. [4]LI M X,MA Z G.Soil moisture drought detection and multi temporal variability across China[J].Science China Earth Sciences,2015,58(10):1798⁃1813. [5]刘慧洁,徐恒,邱文怡,等.bZIP转录因子在植物生长发育及非生物逆境响应的作用[J].浙江农业学报,2019,31(7):1205⁃1214. LIU H J,XU H,QIU W Y,et al.Roles of bZIP transcription factors in plant growth and development and abiotic stress response[J].Acta Agriculturae Zhejiangensis,2019,31(7):1205⁃1214. [6]张桐,李智强,伍国强.WRKY转录因子在植物逆境响应中的作用[J].生物技术通报,2021,37(10):203⁃215. ZHANG T,LI Z Q,WU G Q.Role of WRKY transcription factor in plant response to stresses[J].Biotechnology Bulletin,2021,37(10):203⁃215. [7]PÉREZ⁃RODRÍGUEZ P,RIAÑO⁃PACHÓN D M,CORRÊA L L G,et al.PlnTFDB:Updated content and new features of the plant transcription factor database[J].Nucleic Acids Res,2010,38:822⁃827. [8]牛义岭,姜秀明,许向阳.植物转录因子MYB基因家族的研究进展[J].分子植物育种,2016,14(8):2050⁃2059. NIU Y L,JIANG X M,XU X Y.Research advances on transcription factor MYB gene family in plant[J].Molecular Plant Breeding,2016,14(8):2050⁃2059. [9]JAKOBY M,WEISSHAAR B,DRÖGE⁃LASER W,et al.bZIP transcription factors in Arabidopsis[J].Trends in Plant Science,2002,7(3):106⁃111. [10]LI G J,JIN L,SONG S.Genome⁃wide identification of bHLH transcription factor in Medicago sativa in response to cold stress[J].Genes,2022,13(12):2371. [11]SO H A,LEE J H.NAC Transcription factors from soybean(Glycine max L.)differentially regulated by abiotic stress[J].Journal of Plant Biology,2019,62(2):147⁃160. [12]JIANG J J,MA S H,YE N H,et al.WRKY transcription factors in plant responses to stresses[J].Journal of Integrative Plant Biology,2017,59(2):86⁃101. [13]DRÖGE⁃LASER W,SNOEK B L,SNEL B,et al.The Arabidopsis bZIP transcription factor family:An update[J].Current Opinion in Plant Biology,2018,45:36⁃49. [14]WEI K F,CHEN J,WANG Y M,et al.Genome⁃wide analysis of bZIP⁃encoding genes in maize[J].DNA Research,2012,19(6):463⁃476. [15]LI X Y,GAO S Q,TANG Y M,et al.Genome⁃wide identification and evolutionary analyses of bZIP transcription factors in wheat and its relatives and expression profiles of anther development related TabZIP genes[J].BMC Genomics,2015,16:976⁃996. [16]ZHANG M,LIU Y H,SHI H,et al.Evolutionary and expression analyses of soybean basic leucine zipper transcription factor family[J].BMC Genomics,2018,19(1):159⁃172. [17]邢宇鹏.棉花bZIP基因家族全基因组鉴定及分析[D].泰安:山东农业大学,2020. XING Y P. enome⁃wide identification of the basic leucine zipper(bZIP)gene family in cotton[D].Taian:Shandong Agricultural University,2020. [18]GIBALOVÁ A,REŇÁK D,MATCZUK K,et al.AtbZIP34 is required for Arabidopsis pollen wall patterning and the control of several metabolic pathways in developing pollen[J].Plant Molecular Biology,2009,70(5):581⁃601. [19]VAN LEENE J,BLOMME J,KULKARNI S R,et al.Functional characterization of the Arabidopsis transcription factor bZIP29 reveals its role in leaf and root development[J].Journal of Experimental Botany,2016,67(19):5825⁃5840. [20]MA H Z,LIU C,LI Z X,et al.ZmbZIP4 contributes to stress resistance in maize by regulating ABA synthesis and root development[J].Plant Physiology,2018,178(2):753⁃770. [21]ALEX M G.A bZIP transcription factor accelerates the transition to reproductive tuber growth and aging in Solanum tuberosum[J].Plant Physiology,2022,189(3):1194⁃1195. [22]YANG S H,YAMAGUCHI Y,KOIZUMI N,et al.Promoter analysis of tbzF,a gene encoding a bZIP⁃type transcription factor,reveals distinct variation in cis⁃regions responsible for transcriptional activation between senescing leaves and flower buds in tobacco plants[J].Plant Science,2002,162(6):973⁃980. [23]CHANG C H,LIU Z W,WANG Y Y,et al.A bZIP transcription factor,CaLMF,mediated light⁃regulated camptothecin biosynthesis in Camptotheca acuminata[J].Tree Physiology,2019,39(3):372⁃380. [24]CHANG Y,NGUYEN B H,XIE Y J,et al.Co⁃overexpression of the constitutively active form of OsbZIP46 and ABA activated protein kinase SAPK6 improves drought and temperature stress resistance in rice[J].Frontiers in Plant Science,2017,8:1102. [25]ZHANG Z S,LIU W X,QI X,et al.Genome⁃wide identification,expression profiling,and SSR marker development of the bZIP transcription factor family in Medicago truncatula[J].Biochemical Systematics and Ecology,2015,61:218⁃228. [26]RAMAKRISHNA C,SINGH S,RAGHAVENDRARAO S,et al.The membrane tethered transcription factor EcbZIP17 from finger millet promotes plant growth and enhances tolerance to abiotic stresses[J].Scientific Reports,2018,8(1):2148. [27]HSIEH T H,LI C W,SU R C,et al.A tomato bZIP transcription factor,SlAREB,is involved in water deficit and salt stress response[J].Planta,2010,231(6):1459⁃1473. [28]WANG Z,SU G X,LI M,et al.Overexpressing Arabidopsis ABF3 increases tolerance to multiple abiotic stresses and reduces leaf size in alfalfa[J].Plant Physiology and Biochemistry, 2016, 109:199⁃208. [29]XIANG Y,TANG N,DU H,et al.Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice[J].Plant Physiology,2008,148(4):1938⁃1952. [30]ZHANG X,WANG L,MENG H,et al.Maize ABP9 enhances tolerance to multiple stresses in transgenic Arabidopsis by modulating ABA signaling and cellular levels of reactive oxygen species[J].Plant Molecular Biology,2011,75(4/5):365⁃378. [31]FITZGERALD H A,CANLAS P E,CHERN M S,et al.Alteration of TGA factor activity in rice results in enhanced tolerance to Xanthomonas oryzae pv.oryzae[J].Plant Journal,2005,43(3):335⁃347. [32]LI P,ZHENG T C,LI L L,et al.Genome⁃wide investigation of the bZIP transcription factor gene family in Prunus mume:Classification,evolution,expression profile and low⁃temperature stress responses[J].Horticultural Plant Journal,2021,8(2):230⁃242. [33]KANG S G,PRICE J,LIN P C,et al.The Arabidopsis bZIP1 transcription factor is involved in sugar signaling,protein networking,and DNA binding[J].Molecular Plant,2010,3(2):361⁃373. [34]HU W,YANG H K,YAN Y,et al.Genome⁃wide characterization and analysis of bZIP transcription factor gene family related to abiotic stress in cassava[J].Scientific Reports,2016,6:22783⁃22794. [35]LIU S Y,ZHANG C B,ZHU Q W,et al.Genome⁃ and transcriptome⁃wide systematic characterization of bZIP transcription factor family identifies promising members involved in abiotic stress response in sweetpotato[J].Scientia Horticulturae,2022,303:111185⁃111196. [36]WANG J Z,ZHOU J X,ZHANG B L,et al.Genome⁃wide expansion and expression divergence of the basic leucine zipper transcription factors in higher plants with an emphasis on sorghum[J].Journal of Integrative Plant Biology,2011,53(3):212⁃231. [37]ZHANG Z R,SHAO W Q,NIU J X,et al.Genome⁃wide identification,classification,expression and duplication analysis of bZIP family genes in Juglans regia L[J].International Journal of Molecular Sciences,2022,23(11):5961⁃5978. [38]ZHOU Y,XU D X,JIA L D,et al.Genome⁃wide identification and structural analysis of bZIP transcription factor genes in Brassica napus[J].Genes,2017,8(10):288⁃311. [39]MIRZAEI K, BAHRAMNEJAD B, FATEMI S.Genome⁃wide identification and characterization of the bZIP gene family in potato(Solanum tuberosum)[J].Plant Gene,2020,24:100257⁃100267. [40]张珍珠,陈秀玲,王沛文,等.番茄bZIP基因家族的系统进化分析[J].东北农业大学学报,2014,45(9):47⁃55. ZHANG Z Z,CHEN X L,WANG P W,et al.Phyletic evolution analysis of bZIP family in tomato[J].Journal of Northeast Agricultural University,2014,45(9):47⁃55. [41]ZHAO K,CHEN S,YAO W J,et al.Genome⁃wide analysis and expression profile of the bZIP gene family in poplar[J]. BMC Plant Biology,2021,21(1):122⁃137. [42]LIU C T,WU Y B,WANG X P. bZIP transcription factor OsbZIP52/RISBZ5:A potential negative regulator of cold and drought stress response in rice[J].Planta,2012,235(6):1157⁃1169. [43]ZHANG C Y,LI C,LIU J,et al.The OsABF1 transcription factor improves drought tolerance by activating the transcription of COR413‑TM1 in rice[J].Journal of Experimental Botany,2017,68(16):4695⁃4707. [44]WANG L,CAO H L,QIAN W J,et al.Identification of a novel bZIP transcription factor in Camellia sinensis as a negative regulator of freezing tolerance in transgenic Arabidopsis[J].Annals of Botany,2017,119(7):1195⁃1209. [45]ZOU M J,GUAN Y C,REN H B,et al.A bZIP transcription factor,OsABI5,is involved in rice fertility and stress tolerance[J].Plant Molecular Biology,2008,66(6):675⁃683. [46]TU X M,WANG H X,FENG T X,et al.Expression of a grape(Vitis vinifera) bZIP transcription factor,VlbZIP36,in Arabidopsis thaliana confers tolerance of drought stress during seed germination and seedling establishment[J].Plant Science,2016,252:311⁃323. [47]JOO J,LEE Y H,SONG S I.OsbZIP42 is a positive regulator of ABA signaling and confers drought tolerance to rice[J].Planta,2019,249(5):1521⁃1533. [48]LI X Y,FENG B N,ZHANG F J,et al.Bioinformatic analyses of subgroup⁃A members of the wheat bZIP transcription factor family and functional identification of TabZIP174 involved in drought stress response[J].Frontiers in Plant Science,2016,7:1643⁃1664. [49]ZHU M K,MENG X Q,CAI J,et al.Basic leucine zipper transcription factor SlbZIP1 mediates salt and drought stress tolerance in tomato[J].BMC Plant Biology,2018,18(1):83⁃96. [50]LI Y F,XIAN X,GUO L Q,et al.CsbZIP50 binds to the G⁃box/ABRE motif in CsRD29A promoter to enhance drought tolerance in cucumber[J].Environmental and Experimental Botany,2022,199:104884. [51]FENG Y,WANG Y,ZHANG G F,et al.Group⁃C/S1 bZIP heterodimers regulate MdIPT5b to negatively modulate drought tolerance in apple species[J].The Plant Journal,2021,107(2):399⁃417. [52]HUANG R D.Research progress on plant tolerance to soil salinity and alkalinity in sorghum[J].Journal of Integrative Agriculture,2018,17(4):739⁃746. [53]YOUSEFIRAD S,SOLTANLOO H,RAMEZANPOUR S S,et al.The RNA⁃seq transcriptomic analysis reveals genes mediating salt tolerance through rapid triggering of ion transporters in a mutant barley[J].Public Library of Science One,2020,15(3):e0229513. [54]QU D H,WU F L,ZHAO X H,et al.A bZIP transcription factor VabZIP12 from blueberry induced by dark septate endocyte improving the salt tolerance of transgenic Arabidopsis[J]. Plant Science,2022,315:111135⁃111145. [55]YANG Y,YU T F,MA J,et al.The soybean bZIP transcription factor gene GmbZIP2 confers drought and salt resistances in transgenic plants[J].International Journal of Molecular Sciences,2020,21(2):670⁃688. [56]GAI W X,MA X,QIAO Y M,et al.Characterization of the bZIP transcription factor family in pepper(Capsicum annuum L.) :CabZIP25 positively modulates the salt tolerance[J].Frontiers in Plant Science,2020,11:139. [57]KANG C,ZHAI H,HE S Z,et al.A novel sweetpotato bZIP transcription factor gene,IbbZIP1,is involved in salt and drought tolerance in transgenic Arabidopsis[J].Plant Cell Reports,2019,38(11):1373⁃1382. [58]LI Q,ZHAO H X,WANG X L,et al.Tartary buckwheat transcription factor FtbZIP5,regulated by ftSnRK2.6,can Improve salt/drought resistance in transgenic Arabidopsis[J].International Journal of Molecular Sciences,2020,21(3):1123⁃1138. [59]BI C X,YU Y H,DONG C H,et al.The bZIP transcription factor TabZIP15 improves salt stress tolerance in wheat[J].Plant Biotechnology Journal,2021,19(2):209⁃211. [60]ZHAO P,YE M H,WANG R Q,et al.Systematic identification and functional analysis of potato(Solanum tuberosum L.) bZIP transcription factors and overexpression of potato bZIP transcription factor StbZIP65 enhances salt tolerance[J].International Journal of Biological Macromolecules,2020,161:155⁃167. [61]GENG X L,ZANG X S,LI H R,et al.Unconventional splicing of wheat TabZIP60 confers heat tolerance in transgenic Arabidopsis[J].Plant Science,2018,274:252⁃260. [62]XIE C,YANG L,JIA G X,et al.Maize heat up⁃regulated gene 1 plays vital roles in heat stress tolerance[J].Journal of Experimental Botany,2022,73(18):6417⁃6433. [63]KAUR N,KANDOTH K P.Tomato bZIP60 mRNA undergoes splicing in endoplasmic reticulum stress and in response to environmental stresses[J].Plant Physiology and Biochemistry,2021,160:397⁃403. [64]耿晓丽,臧新山,王飞,等.小麦耐热相关转录因子基因TabZIP28的分离及功能分析[J].农业生物技术学报,2016,24(2):157⁃167. GENG X L,ZANG X S,WANG F,et al.Isolation and function analysis of heat stress related transcription factor gene TabZIP28 in wheat(Triticum aestivum)[J].Journal of Agricultural Biotechnology,2016,24(2):157⁃167. [65]SAMTANI H,SHARMA A,KHURANA P.Wheat ocs⁃element binding factor 1 enhances thermotolerance by modulating the heat stress response pathway[J].Frontiers in Plant Science,2022,13:914363. [66]LIANG Y,XIA J Q,JIANG Y S,et al.Genome⁃wide identification and analysis of bZIP gene family and resistance of TaABI5(TabZIP96)under freezing stress in wheat(Triticum aestivum)[J].International Journal of Molecular Sciences,2022,23(4):2351⁃2371. [67]CAI W T,YANG Y L,WANG W W,et al.Overexpression of a wheat(Triticum aestivum L.)bZIP transcription factor gene,TabZIP6,decreased the freezing tolerance of transgenic Arabidopsis seedlings by down⁃regulating the expression of CBFs[J].Plant Physiology and Biochemistry,2018,124:100⁃111. [68]LI Z Y,FU D Y,WANG X,et al.The transcription factor bZIP68 negatively regulates cold tolerance in maize[J].The Plant Cell,2022,34(8):2833⁃2851. [69]YAO L N,HAO X Y,CAO H L,et al.ABA⁃dependent bZIP transcription factor,CsbZIP18,from Camellia sinensis negatively regulates freezing tolerance in Arabidopsis[J].Plant Cell Reports,2020,39(4):553⁃565. [70]AN J P,YAO J F,WANG X N,et al.MdHY5 positively regulates cold tolerance via CBF⁃dependent and CBF⁃independent pathways in apple[J].Journal of Plant Physiology,2017,218:275⁃281. [71]BAI H R,LIAO X Q,LI X,et al.DgbZIP3 interacts with DgbZIP2 to increase the expression of DgPOD for cold stress tolerance in chrysanthemum [J].Horticulture Research,2022,9:uhac105. [72]王媛媛.bZIP44基因在植物缺铁响应中的作用研究[D].合肥:合肥工业大学,2019. WANG Y Y.The role of bZIP44 gene in plant response to iron deficiency[D].Hefei:Hefei University of Technology,2019. [73]INABA S,KURATA R,KOBAYASHI M,et al.Identification of putative target genes of bZIP19,a transcription factor essential for Arabidopsis adaptation to Zn deficiency in roots[J].Plant Journal,2015,84(2):323⁃334. [74]ASSUNÇÃO A G,HERRERO E,LIN Y F,et al.Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency[J].Proceedings of the National Academy of Sciences,2010,107(22):10296⁃10301. [75]FENG Z,HUANG J C,HAO G,et al.OsRLCK160 contributes to flavonoid accumulation and UV⁃B tolerance by regulating OsbZIP48 in rice[J].Science China(Life Sciences),2022,65(7):1380⁃1394. [76]LING B Q,BAI X X,ZHOU Y B,et al.Functional analysis of AtTGA4 transgenic wheat tolerance to low phosphorus stress in field[J].Scientia Agricultura Sinica,2018,51(12):2225⁃2234. [77]YU Y H,JIAO Z L,BIAN L,et al.Overexpression of Vitis vinifera VvbZIP60 enhances Arabidopsis resistance to powdery mildew via the salicylic acid signaling pathway[J].Scientia Horticulturae,2019,256:108640⁃108646. [78]XU Y P,WANG Y C,ZHAO H Z,et al.Genome⁃wide identification and expression analysis of the bZIP transcription factors in the mycoparasite Coniothyrium minitans[J].Microorganisms,2020,8(7):1045⁃1064. [79]ZHANG Y,ZHANG G,XIA N,et al.Cloning and characterization of a bZIP transcription factor gene in wheat and its expression in response to stripe rust pathogen infection and abiotic stresses[J].Physiological and Molecular Plant Pathology,2008,73(4):88⁃94. [80]ZHANG M,LIU Y H,LI Z X,et al.The bZIP transcription factor GmbZIP15 facilitates resistance against Sclerotinia sclerotiorum and Phytophthora sojae infection in soybean[J].iScience,2021,24(6):102642⁃102662. [81]YANG S S,ZHANG X X,ZHANG X M,et al.A bZIP transcription factor,PqbZIP1,is involved in the plant defense response of American ginseng[J].PeerJ,2022,10:e12939. [82]LEE S C,CHOI H W,HWANG I S,et al.Functional roles of the pepper pathogen⁃induced bZIP transcription factor,CAbZIP1,in enhanced resistance to pathogen infection and environmental stresses[J].Planta,2006,224(5):1209⁃1225. [83]MANAVELLA P A,DEZAR C A,BONAVENTURE G,et al.HAHB4,a sunflower HD⁃Zip protein,integrates signals from the jasmonic acid and ethylene pathways during wounding and biotic stress responses[J].The Plant Journal,2008,56(3):376⁃388. [84]GAO D,APPIANO M,HUIBERS R P,et al.Activation tagging of ATHB13 in Arabidopsis thaliana confers broad⁃spectrum disease resistance[J].Plant Molecular Biology,2014,86(6):641⁃653. |
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