河南农业科学 ›› 2022, Vol. 51 ›› Issue (12): 90-96.DOI: 10.15933/j.cnki.1004-3268.2022.12.011

• 植物保护 • 上一篇    下一篇

河南烟草病毒病田间发生动态及种类监测分析

白静科1,吴彦辉2,牛龙龙1,郝浩浩3,王雪芬4,张晓丽5,李淑君1   

  1. (1.河南省农业科学院烟草研究所/烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南 许昌 461000;2.河南省烟草公司许昌市公司,河南 许昌 461000;3.河南省烟草公司驻马店市公司,河南 驻马店 463000;4.许昌市烟草公司襄城县分公司,河南 襄城 461700;5.三门峡市烟草公司,河南 三门峡 472000)
  • 收稿日期:2022-05-25 出版日期:2022-12-15 发布日期:2023-01-17
  • 通讯作者: 李淑君(1966-),女,河南沁阳人,研究员,硕士,主要从事烟草植物保护研究。E-mail:13603749396@126.com
  • 作者简介:白静科(1990-),女,河南安阳人,助理研究员,硕士,主要从事烟草植物保护研究。E-mail:baijingke@126.com
  • 基金资助:
    许昌市烟草公司科技项目(2021411000240104);河南省烟草公司科技项目(2020410000270011);河南省农业科学院科技创新团队项目(2022TD26)

Research on the Occurrence in Fields and Detection of Tobacco Viruses in Henan Province

BAI Jingke1,WU Yanhui2,NIU Longlong1,HAO Haohao3,WANG Xuefen4,ZHANG Xiaoli5,LI Shujun1   

  1. (1.Tobacco Research Institute,Henan Academy of Agricultural Sciences/Key Laboratory for Green Preservation&Control of Tobacco Diseases and Pests in Huanghuai Growing Area,Xuchang 461000,China;2.Xuchang Branch of Henan Provincial Tobacco Company,Xuchang 461000,China;3.Zhumadian Branch of Henan Provincial Tobacco Company,Zhumadian 463000,China;4.Xiangcheng County Branch of Xuchang Tobacco Company,Xiangcheng 461700,China;5.Sanmenxia Branch of Henan Provincial Tobacco Company,Sanmenxia 472000,China)
  • Received:2022-05-25 Published:2022-12-15 Online:2023-01-17

摘要: 烟草病毒病是影响烟草生长的一种重要病害,其发生具有周期性、区域性等特点。为明确河南烟区近年来病毒病田间发生动态和主要病毒种类变化,采用系统调查方法调查豫南、豫中、豫西烟区病毒病田间发生动态,并利用酶联免疫吸附法检测采自豫南、豫中、豫西烟区的病毒病样本。结果显示,烟草病毒病的田间发生动态曲线呈S形,初发期是在烟草移栽后的25~30 d,60~70 d后达到高峰期,烟株成熟后进入稳定期。研究结果还显示,2017—2021 年河南烟区主要病毒种类是烟草普通花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、烟草蚀纹病毒(Tobacco etchvirus,TEV)、烟草脉带花叶病毒(Tobacco vein banding mosaic virus,TVBMV)和马铃薯Y 病毒(Potato virus Y,PVY)。

关键词: 烟草病毒病, 河南烟区, 田间发生动态, 种类分析, ELISA检测

Abstract: Tobacco virus disease is an important disease affecting tobacco growth,which occurs periodically and regionally.To clarify the occurrence in fields and variation of tobacco viruses in Henan Province in recent years,we systematically investigated the dynamics of tobacco viruses in the southern,central and western regions of Henan respectively,and detected tobacco viruses samples by ELISA simultaneously. The results indicated that the dynamic curve of tobacco virus disease in field was S‑shaped,with a initial stage appearing at 25—30 days after transplanting,then a peak emerging after 60—70 days,and at last reaching a stable stage when plants were ripe.It also showed that the main virus species were tobacco mosaic virus(TMV),cucumber mosaic virus(CMV),tobacco etch virus(TEV),tobacco vein banding mosaic virus(TVBMV)and potato virus Y(PVY)from 2017 to 2021.

Key words: Tobacco virus disease, Henan tobacco?growing area, Occurrence in fields, Species analysis, ELISA detection

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