关键词: 水稻, 叶片, 简便烘干法, DNA大量提取, 液氮冷冻法

Abstract: In order to explore the feasibility of drying method instead of liquid nitrogen freezing method to grind rice leaves to extract DNA,firstly the amplification effects of DNA extracted from grinded rice leaves by low temperature drying under 50℃ and liquid nitrogen freezing were compared,and then the amplification effects of DNA extracted from leaf grinded by drying method under different temperature (50,60,70,80,90℃ ) were further analyzed to screen the best drying temperature.The results showed that the DNA extracted from leaf grinded by drying method under 50—70℃ could be effectively amplified,the effect was the best under 50℃ ,and there was no significant difference between the amplification effects of the DNA extracted from leaf grinded by drying method and liquid nitrogen freezing method.The drying method of rice leaves had many advantages,such as simple operation,low cost and strong practicability,which could replace the liquid nitrogen freezing method to prepare the leaves in the large-scale extraction of DNA,and had good popularization and application value.

Key words: Rice, Leaf, Simple drying method, Extraction of massive DNA, Liquid nitrogen freezing method